Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study
Antioxidant activity and antihyperglycemic constituents and of traditional medicinal plants are currently the preferred therapeutic means of treatment and management of diabetes because of the undesired adverse effect of synthetic drugs. Muntingia calabura (Kerukupsiam) leaves and other parts are co...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Results in Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715624000018 |
| _version_ | 1797359965257596928 |
|---|---|
| author | Ahmad Anas Nagoor Gunny Nimaal K. Prammakumar Abdul Aziz Ahmad Subash C.B. Gopinath Amirul Ridzuan Abu Bakar Haliru Musa Mohd Hishamuddin Che Mat |
| author_facet | Ahmad Anas Nagoor Gunny Nimaal K. Prammakumar Abdul Aziz Ahmad Subash C.B. Gopinath Amirul Ridzuan Abu Bakar Haliru Musa Mohd Hishamuddin Che Mat |
| author_sort | Ahmad Anas Nagoor Gunny |
| collection | DOAJ |
| description | Antioxidant activity and antihyperglycemic constituents and of traditional medicinal plants are currently the preferred therapeutic means of treatment and management of diabetes because of the undesired adverse effect of synthetic drugs. Muntingia calabura (Kerukupsiam) leaves and other parts are considered as alternative natural sources of treatment for diabetes. Ultrasonic assisted extraction is a novel approach for extraction of phytoconstituents which gives high extraction yield of bioactive compounds. However, there has been no published information presently on the use of ethanol ultrasonic assisted extraction method for assessment of antioxidant and antidiabetic activities of M. calabura leaves. Hence, the current study aims to evaluate the in vitro antioxidant and antidiabetic activities of M. calabura leave extract. IC50 analysis was done to determine theinhibitory concentration and the results obtained from 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay showed IC50 of gallic acid to be 1.0 µg/ml, which is lower than M. calabura leaves extract at 2.54 µg/ml, indicating that only small concentration of gallic acid was required to inhibit the free radicals at 50 %. However, IC50 analysis for amylase inhibition showed that M. calabura extract had 44.39 µg/ml antidiabetic activity compared to acarbose with 57.1 µg/ml activity. This indicates that M. calabura leaves extract has a better inhibition on amylase activity compared to the acarbose which is a synthetic drug. Further still, in silico study was carried out and the molecular docking result of eight ligands against amylase indicates quercetin had the least binding free energy of −9.1 kcal/mol, indicating the strongest interaction. Using Lineweaver-Burk plot, the results showed a competitive inhibition, hence, it was justified that M. calabura has the potential to manage diabetes and other diseases related to free radicals. |
| first_indexed | 2024-03-08T15:31:33Z |
| format | Article |
| id | doaj.art-5fcf6d13505e47dc92302496ff26b05a |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-03-08T15:31:33Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-5fcf6d13505e47dc92302496ff26b05a2024-01-10T04:36:13ZengElsevierResults in Chemistry2211-71562024-01-017101305Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking studyAhmad Anas Nagoor Gunny0Nimaal K. Prammakumar1Abdul Aziz Ahmad2Subash C.B. Gopinath3Amirul Ridzuan Abu Bakar4Haliru Musa5Mohd Hishamuddin Che Mat6Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Kompleks Pusat Pengajian, Jejawi 3, Arau, Perlis, Malaysia; Centre of Excellence for Biomass Utilization, Universiti Malaysia Perlis (UniMAP), 02600 Arau, Perlis, Malaysia; Corresponding author at: Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3, Arau, Perlis, Malaysia.Faculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Kompleks Pusat Pengajian, Jejawi 3, Arau, Perlis, MalaysiaDepartment of Science in Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, Jalan Gombak, Selangor, MalaysiaFaculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Kompleks Pusat Pengajian, Jejawi 3, Arau, Perlis, Malaysia; Institute of Nano Electronic Engineering, Universiti Malaysia Perlis (UniMAP), 01000 Kangar, Perlis, Malaysia; Micro System Technology, Centre of Excellence (CoE), Universiti Malaysia Perlis (UniMAP), Pauh Campus, 02600 Arau, Perlis, MalaysiaFaculty of Chemical Engineering & Technology, Universiti Malaysia Perlis (UniMAP), Kompleks Pusat Pengajian, Jejawi 3, Arau, Perlis, Malaysia; Centre of Excellence for Biomass Utilization, Universiti Malaysia Perlis (UniMAP), 02600 Arau, Perlis, MalaysiaDepartment of Microbiology, Faculty of Natural and Applied Sciences, Veritas University, Bwari Area Council, Abuja, NigeriaCentre of Excellence for Biomass Utilization, Universiti Malaysia Perlis (UniMAP), 02600 Arau, Perlis, Malaysia; Institute of Sustainable Agrotechnology, Universiti Malaysia Perlis, Sg. Chuchuh Campus, 02100 Padang Besar, Perlis, MalaysiaAntioxidant activity and antihyperglycemic constituents and of traditional medicinal plants are currently the preferred therapeutic means of treatment and management of diabetes because of the undesired adverse effect of synthetic drugs. Muntingia calabura (Kerukupsiam) leaves and other parts are considered as alternative natural sources of treatment for diabetes. Ultrasonic assisted extraction is a novel approach for extraction of phytoconstituents which gives high extraction yield of bioactive compounds. However, there has been no published information presently on the use of ethanol ultrasonic assisted extraction method for assessment of antioxidant and antidiabetic activities of M. calabura leaves. Hence, the current study aims to evaluate the in vitro antioxidant and antidiabetic activities of M. calabura leave extract. IC50 analysis was done to determine theinhibitory concentration and the results obtained from 2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay showed IC50 of gallic acid to be 1.0 µg/ml, which is lower than M. calabura leaves extract at 2.54 µg/ml, indicating that only small concentration of gallic acid was required to inhibit the free radicals at 50 %. However, IC50 analysis for amylase inhibition showed that M. calabura extract had 44.39 µg/ml antidiabetic activity compared to acarbose with 57.1 µg/ml activity. This indicates that M. calabura leaves extract has a better inhibition on amylase activity compared to the acarbose which is a synthetic drug. Further still, in silico study was carried out and the molecular docking result of eight ligands against amylase indicates quercetin had the least binding free energy of −9.1 kcal/mol, indicating the strongest interaction. Using Lineweaver-Burk plot, the results showed a competitive inhibition, hence, it was justified that M. calabura has the potential to manage diabetes and other diseases related to free radicals.http://www.sciencedirect.com/science/article/pii/S2211715624000018DiabetesMedicinal PlantAmylase InhibitorLineweaver-BurkIn silico |
| spellingShingle | Ahmad Anas Nagoor Gunny Nimaal K. Prammakumar Abdul Aziz Ahmad Subash C.B. Gopinath Amirul Ridzuan Abu Bakar Haliru Musa Mohd Hishamuddin Che Mat Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study Results in Chemistry Diabetes Medicinal Plant Amylase Inhibitor Lineweaver-Burk In silico |
| title | Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study |
| title_full | Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study |
| title_fullStr | Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study |
| title_full_unstemmed | Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study |
| title_short | Exploring antioxidant and antidiabetic potential of Mutingia calabura (Kerukupsiam) leaf extract: In vitro analysis and molecular docking study |
| title_sort | exploring antioxidant and antidiabetic potential of mutingia calabura kerukupsiam leaf extract in vitro analysis and molecular docking study |
| topic | Diabetes Medicinal Plant Amylase Inhibitor Lineweaver-Burk In silico |
| url | http://www.sciencedirect.com/science/article/pii/S2211715624000018 |
| work_keys_str_mv | AT ahmadanasnagoorgunny exploringantioxidantandantidiabeticpotentialofmutingiacalaburakerukupsiamleafextractinvitroanalysisandmoleculardockingstudy AT nimaalkprammakumar exploringantioxidantandantidiabeticpotentialofmutingiacalaburakerukupsiamleafextractinvitroanalysisandmoleculardockingstudy AT abdulazizahmad exploringantioxidantandantidiabeticpotentialofmutingiacalaburakerukupsiamleafextractinvitroanalysisandmoleculardockingstudy AT subashcbgopinath exploringantioxidantandantidiabeticpotentialofmutingiacalaburakerukupsiamleafextractinvitroanalysisandmoleculardockingstudy AT amirulridzuanabubakar exploringantioxidantandantidiabeticpotentialofmutingiacalaburakerukupsiamleafextractinvitroanalysisandmoleculardockingstudy AT halirumusa exploringantioxidantandantidiabeticpotentialofmutingiacalaburakerukupsiamleafextractinvitroanalysisandmoleculardockingstudy AT mohdhishamuddinchemat exploringantioxidantandantidiabeticpotentialofmutingiacalaburakerukupsiamleafextractinvitroanalysisandmoleculardockingstudy |