Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis
Angipoietin‐1 (Angpt1) and von Willebrand factor (VWF) are two important angiogenic molecules that can drive pathologic angiogenesis and progression of liver fibrosis in our previous study. MicroRNAs (miRs) participate in a variety of physiological and pathological processes, including angiogenesis....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Health/LWW
2022-06-01
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| Series: | Hepatology Communications |
| Online Access: | https://doi.org/10.1002/hep4.1888 |
| _version_ | 1797756843466948608 |
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| author | Le Yang Wenhui Yue Hang Zhang Zhi Zhang Renmin Xue Chengbin Dong Fuquan Liu Na Chang Lin Yang Liying Li |
| author_facet | Le Yang Wenhui Yue Hang Zhang Zhi Zhang Renmin Xue Chengbin Dong Fuquan Liu Na Chang Lin Yang Liying Li |
| author_sort | Le Yang |
| collection | DOAJ |
| description | Angipoietin‐1 (Angpt1) and von Willebrand factor (VWF) are two important angiogenic molecules that can drive pathologic angiogenesis and progression of liver fibrosis in our previous study. MicroRNAs (miRs) participate in a variety of physiological and pathological processes, including angiogenesis. However, the critical miRs targeting Angpt1 or VWF and potential molecular mechanism underlying liver fibrosis–associated angiogenesis is not clear yet. Human liver tissues were obtained from patients with different chronic liver diseases. Mouse models of liver fibrosis were induced by injection of CCl4 or bile duct ligation (BDL) operation. MiR‐671‐5p was predicted to target Angpt1 and VWF from three databases (miRanda, RNA22v2, and miRwalk). MiR‐671‐5p expression was decreased in the fibrotic liver of human and mice, with a negative correlation with the levels of Angpt1, VWF, sphingosine kinase‐1 (SphK1, the rate‐limiting enzyme for sphingosine 1‐phosphate [S1P] formation), transforming growth factor β1 (TGFβ1), hypoxia inducible factor (Hif)1α, Hif2α, and fibrosis markers. Importantly, miR‐671‐5p expression was down‐regulated in fluorescence‐activated cell sorted liver sinusoidal endothelial cells and hepatic stellate cells (HSCs) in CCl4 mice compared with control mice. In vitro miR‐671‐5p expression was also decreased in S1P‐stimulated HSCs and TGFβ1‐activated liver sinusoidal endothelial cells, negatively correlated with Angpt1 and VWF expression. MiR‐671‐5p directly targeted Angpt1 and VWF by luciferase reporter assays. In vivo administration of miR‐671‐5p agomir decreased the messenger RNA and protein levels of Anpgt1 and VWF, and attenuated CCl4‐induced or BDL‐induced liver angiogenesis and fibrosis. Conclusion: We identify the negative regulation of miR‐671‐5p on Angpt1 and VWF and liver fibrosis–associated angiogenesis, which may provide promising targets for the prevention and treatment of liver disease. |
| first_indexed | 2024-03-12T18:07:23Z |
| format | Article |
| id | doaj.art-5fd08d666f7f4d5f8c04cbb9f48df169 |
| institution | Directory Open Access Journal |
| issn | 2471-254X |
| language | English |
| last_indexed | 2024-03-12T18:07:23Z |
| publishDate | 2022-06-01 |
| publisher | Wolters Kluwer Health/LWW |
| record_format | Article |
| series | Hepatology Communications |
| spelling | doaj.art-5fd08d666f7f4d5f8c04cbb9f48df1692023-08-02T09:26:02ZengWolters Kluwer Health/LWWHepatology Communications2471-254X2022-06-01661425144210.1002/hep4.1888Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and FibrosisLe Yang0Wenhui Yue1Hang Zhang2Zhi Zhang3Renmin Xue4Chengbin Dong5Fuquan Liu6Na Chang7Lin Yang8Liying Li9Department of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaDepartment of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaDepartment of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaDepartment of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaDepartment of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaDepartment of Interventional Therapy Beijing Shijitan HospitalCapital Medical University Beijing ChinaDepartment of Interventional Therapy Beijing Shijitan HospitalCapital Medical University Beijing ChinaDepartment of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaDepartment of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaDepartment of Cell Biology Municipal Laboratory for Liver Protection and Regulation of Regeneration Capital Medical University Beijing ChinaAngipoietin‐1 (Angpt1) and von Willebrand factor (VWF) are two important angiogenic molecules that can drive pathologic angiogenesis and progression of liver fibrosis in our previous study. MicroRNAs (miRs) participate in a variety of physiological and pathological processes, including angiogenesis. However, the critical miRs targeting Angpt1 or VWF and potential molecular mechanism underlying liver fibrosis–associated angiogenesis is not clear yet. Human liver tissues were obtained from patients with different chronic liver diseases. Mouse models of liver fibrosis were induced by injection of CCl4 or bile duct ligation (BDL) operation. MiR‐671‐5p was predicted to target Angpt1 and VWF from three databases (miRanda, RNA22v2, and miRwalk). MiR‐671‐5p expression was decreased in the fibrotic liver of human and mice, with a negative correlation with the levels of Angpt1, VWF, sphingosine kinase‐1 (SphK1, the rate‐limiting enzyme for sphingosine 1‐phosphate [S1P] formation), transforming growth factor β1 (TGFβ1), hypoxia inducible factor (Hif)1α, Hif2α, and fibrosis markers. Importantly, miR‐671‐5p expression was down‐regulated in fluorescence‐activated cell sorted liver sinusoidal endothelial cells and hepatic stellate cells (HSCs) in CCl4 mice compared with control mice. In vitro miR‐671‐5p expression was also decreased in S1P‐stimulated HSCs and TGFβ1‐activated liver sinusoidal endothelial cells, negatively correlated with Angpt1 and VWF expression. MiR‐671‐5p directly targeted Angpt1 and VWF by luciferase reporter assays. In vivo administration of miR‐671‐5p agomir decreased the messenger RNA and protein levels of Anpgt1 and VWF, and attenuated CCl4‐induced or BDL‐induced liver angiogenesis and fibrosis. Conclusion: We identify the negative regulation of miR‐671‐5p on Angpt1 and VWF and liver fibrosis–associated angiogenesis, which may provide promising targets for the prevention and treatment of liver disease.https://doi.org/10.1002/hep4.1888 |
| spellingShingle | Le Yang Wenhui Yue Hang Zhang Zhi Zhang Renmin Xue Chengbin Dong Fuquan Liu Na Chang Lin Yang Liying Li Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis Hepatology Communications |
| title | Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis |
| title_full | Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis |
| title_fullStr | Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis |
| title_full_unstemmed | Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis |
| title_short | Dual Targeting of Angipoietin‐1 and von Willebrand Factor by microRNA‐671‐5p Attenuates Liver Angiogenesis and Fibrosis |
| title_sort | dual targeting of angipoietin 1 and von willebrand factor by microrna 671 5p attenuates liver angiogenesis and fibrosis |
| url | https://doi.org/10.1002/hep4.1888 |
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