Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites
Objective The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were se...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2022-10-01
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| Series: | Animal Bioscience |
| Subjects: | |
| Online Access: | http://www.animbiosci.org/upload/pdf/ab-21-0411.pdf |
| _version_ | 1818066198312517632 |
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| author | Wen Zhu Tianwei Liu Jian Deng Cong Cong Wei Zi Jun Zhang Di Ming Wang Xing Yong Chen |
| author_facet | Wen Zhu Tianwei Liu Jian Deng Cong Cong Wei Zi Jun Zhang Di Ming Wang Xing Yong Chen |
| author_sort | Wen Zhu |
| collection | DOAJ |
| description | Objective The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet. |
| first_indexed | 2024-12-10T15:03:58Z |
| format | Article |
| id | doaj.art-5fd3c8035ab447deab8601dd3134f27a |
| institution | Directory Open Access Journal |
| issn | 2765-0189 2765-0235 |
| language | English |
| last_indexed | 2024-12-10T15:03:58Z |
| publishDate | 2022-10-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj.art-5fd3c8035ab447deab8601dd3134f27a2022-12-22T01:44:06ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352022-10-0135101535154410.5713/ab.21.041124860Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolitesWen Zhu0Tianwei Liu1Jian Deng2Cong Cong Wei3Zi Jun Zhang4Di Ming Wang5Xing Yong Chen6 College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China Key Laboratory of Molecular Animal Nutirtion, Ministry of Education, Zhejiang University, Hangzhou 310058, China College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, ChinaObjective The objective of this study was to investigate the effects of decreasing dietary crude protein content on rumen fermentation, mictobiota, and metabolites in goats. Methods In an 84-day feeding trial, a total of twelve male Anhui white goat kids with initial body weight 15.9±1.13 kg were selected and randomly classified into two groups, feeding a normal crude protein diet (14.8% CP, NCP) or a low crude protein diet (12.0% CP, LCP). At the end of the experimental trial (on day 84), six animals were randomly selected from each group and were slaughtered to collect rumen fluid samples for the analysis of rumen fermentation parameters, microbiome, and metabolome. Results The concentrations of ammonia-nitrogen, total volatile fatty acid, acetate, and propionate were decreased (p<0.05) in the LCP group in comparison with those in the NCP group. The abundances of genera Prevotella, Campylobacter, Synergistetes, and TG5, which were associated with nitrogen metabolism, were lower (p<0.05) in the LCP group compared with those in the NCP group. The levels of 78 metabolites (74 decreased, 4 increased) in the rumen fluid were altered (p<0.05) by the treatment. Most of the ruminal metabolites that showed decreased levels in the LCP group were substrates for microbial protein synthesis. Metabolic pathway analysis showed that vitamin B6 metabolism was significantly different (p<0.05) in rumen fluid between the two treatments. Conclusion Decreased dietary protein level inhibited rumen fermentation through microbiome and metabolome shifts in goat kids. These results enhance our understanding of ruminal bacteria and metabolites of goat fed a low protein diet.http://www.animbiosci.org/upload/pdf/ab-21-0411.pdfgoatlow proteinmetabolomicmicrobiomerumen |
| spellingShingle | Wen Zhu Tianwei Liu Jian Deng Cong Cong Wei Zi Jun Zhang Di Ming Wang Xing Yong Chen Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites Animal Bioscience goat low protein metabolomic microbiome rumen |
| title | Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites |
| title_full | Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites |
| title_fullStr | Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites |
| title_full_unstemmed | Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites |
| title_short | Microbiome-metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites |
| title_sort | microbiome metabolomics analysis of the effects of decreasing dietary crude protein content on goat rumen mictobiota and metabolites |
| topic | goat low protein metabolomic microbiome rumen |
| url | http://www.animbiosci.org/upload/pdf/ab-21-0411.pdf |
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