Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury
Background Krüppel-like factor 6 (KLF6) is a transcription factor that participate in various pathophysiological processes, but its contribution in ischemia acute kidney injury (AKI) is lacking so far. The study aimed to investigate the expression and the role of KLF6 in kidney ischemia–reperfusion...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Renal Failure |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/0886022X.2020.1793353 |
| _version_ | 1818737610017734656 |
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| author | Dan Li Xiaoqiang Liu Chenyu Li Yue Zhang Chen Guan Junyan Huang Yan Xu |
| author_facet | Dan Li Xiaoqiang Liu Chenyu Li Yue Zhang Chen Guan Junyan Huang Yan Xu |
| author_sort | Dan Li |
| collection | DOAJ |
| description | Background Krüppel-like factor 6 (KLF6) is a transcription factor that participate in various pathophysiological processes, but its contribution in ischemia acute kidney injury (AKI) is lacking so far. The study aimed to investigate the expression and the role of KLF6 in kidney ischemia–reperfusion (IR) injury. Method Microarray data were collected from GSE58438 and GSE52004. The rat IR model was established to evaluate the mRNA and protein expression of KLF6 and inflammatory cytokines in serum and kidney tissues. SiRNA-KLF6 was transfected with HK-2 cells, and then a cell-based hypoxia-reoxygenation (HR) model was established. Results Bioinformatics showed KLF6 mRNA in kidney tissue is up-regulated in 3 h after IR in rat kidney, which involved in cell activation, leukocyte activation, and response to hydrogen peroxide after IR. The rat IR model results showed that KLF6 expression was peaking at 6 h, and the expression of pro-inflammatory cytokines MCP-1 and TNF-α was increased both in serum and kidney tissues, while anti-inflammatory cytokine IL-10 was decreased after IR. Furthermore, in vitro results showed that KLF6 knock-down reduced the pro-inflammatory cytokines expression. Conclusion These results suggest that (1) KLF6 might be a novel biomarker for early diagnosis of AKI and (2) KLF6 may play a role in promoting inflammation in AKI. |
| first_indexed | 2024-12-18T00:55:46Z |
| format | Article |
| id | doaj.art-5fd40300478f411795e8d605380f245d |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-12-18T00:55:46Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-5fd40300478f411795e8d605380f245d2022-12-21T21:26:32ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492020-01-0142169370310.1080/0886022X.2020.17933531793353Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injuryDan Li0Xiaoqiang Liu1Chenyu Li2Yue Zhang3Chen Guan4Junyan Huang5Yan Xu6Department of Nephrology, The Affiliated Hospital of Qingdao UniversityReproductive Medicine Center, Qingdao Women and Children's HospitalDepartment of Nephrology, The Affiliated Hospital of Qingdao UniversityDepartment of Nephrology, The Affiliated Hospital of Qingdao UniversityDepartment of Nephrology, The Affiliated Hospital of Qingdao UniversityDepartment of Nephrology, Qingdao Central HospitalDepartment of Nephrology, The Affiliated Hospital of Qingdao UniversityBackground Krüppel-like factor 6 (KLF6) is a transcription factor that participate in various pathophysiological processes, but its contribution in ischemia acute kidney injury (AKI) is lacking so far. The study aimed to investigate the expression and the role of KLF6 in kidney ischemia–reperfusion (IR) injury. Method Microarray data were collected from GSE58438 and GSE52004. The rat IR model was established to evaluate the mRNA and protein expression of KLF6 and inflammatory cytokines in serum and kidney tissues. SiRNA-KLF6 was transfected with HK-2 cells, and then a cell-based hypoxia-reoxygenation (HR) model was established. Results Bioinformatics showed KLF6 mRNA in kidney tissue is up-regulated in 3 h after IR in rat kidney, which involved in cell activation, leukocyte activation, and response to hydrogen peroxide after IR. The rat IR model results showed that KLF6 expression was peaking at 6 h, and the expression of pro-inflammatory cytokines MCP-1 and TNF-α was increased both in serum and kidney tissues, while anti-inflammatory cytokine IL-10 was decreased after IR. Furthermore, in vitro results showed that KLF6 knock-down reduced the pro-inflammatory cytokines expression. Conclusion These results suggest that (1) KLF6 might be a novel biomarker for early diagnosis of AKI and (2) KLF6 may play a role in promoting inflammation in AKI.http://dx.doi.org/10.1080/0886022X.2020.1793353krüppel-like factor 6acute kidney injuryischemia–reperfusion injuryproximal tubule epithelial cellsinflammation |
| spellingShingle | Dan Li Xiaoqiang Liu Chenyu Li Yue Zhang Chen Guan Junyan Huang Yan Xu Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury Renal Failure krüppel-like factor 6 acute kidney injury ischemia–reperfusion injury proximal tubule epithelial cells inflammation |
| title | Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury |
| title_full | Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury |
| title_fullStr | Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury |
| title_full_unstemmed | Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury |
| title_short | Role of promoting inflammation of Krüppel-like factor 6 in acute kidney injury |
| title_sort | role of promoting inflammation of kruppel like factor 6 in acute kidney injury |
| topic | krüppel-like factor 6 acute kidney injury ischemia–reperfusion injury proximal tubule epithelial cells inflammation |
| url | http://dx.doi.org/10.1080/0886022X.2020.1793353 |
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