Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli?
Giardia duodenalis is a protozoan parasite responsible for giardiasis, a disease characterized by intestinal malabsorption, diarrhea and abdominal pain in a large number of mammal species. Giardiasis is one of the most common intestinal parasitic diseases in the world and thus a high veterinary, and...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00089/full |
| _version_ | 1819180033338507264 |
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| author | Thibault Allain Thibault Allain Soraya Chaouch Myriam Thomas Myriam Thomas Marie-Agnès Travers Isabelle Valle Isabelle Valle Philippe Langella Philippe Grellier Bruno Polack Bruno Polack Isabelle Florent Luis G. Bermúdez-Humarán |
| author_facet | Thibault Allain Thibault Allain Soraya Chaouch Myriam Thomas Myriam Thomas Marie-Agnès Travers Isabelle Valle Isabelle Valle Philippe Langella Philippe Grellier Bruno Polack Bruno Polack Isabelle Florent Luis G. Bermúdez-Humarán |
| author_sort | Thibault Allain |
| collection | DOAJ |
| description | Giardia duodenalis is a protozoan parasite responsible for giardiasis, a disease characterized by intestinal malabsorption, diarrhea and abdominal pain in a large number of mammal species. Giardiasis is one of the most common intestinal parasitic diseases in the world and thus a high veterinary, and public health concern. It is well-established that some probiotic bacteria may confer protection against this parasite in vitro and in vivo and we recently documented the implication of bile-salt hydrolase (BSH)-like activities from strain La1 of Lactobacillus johnsonii as mediators of these effects in vitro. We showed that these activities were able to generate deconjugated bile salts that were toxic to the parasite. In the present study, a wide collection of lactobacilli strains from different ecological origins was screened to assay their anti-giardial effects. Our results revealed that the anti-parasitic effects of some of the strains tested were well-correlated with the expression of BSH-like activities. The two most active strains in vitro, La1 and Lactobacillus gasseri CNCM I-4884, were then tested for their capacity to influence G. duodenalis infection in a suckling mice model. Strikingly, only L. gasseri CNCM I-4884 strain was able to significantly antagonize parasite growth with a dramatic reduction of the trophozoites load in the small intestine. Moreover, this strain also significantly reduced the fecal excretion of Giardia cysts after 5 days of treatment, which could contribute to blocking the transmission of the parasite, in contrast of La1 where no effect was observed. This study represents a step toward the development of new prophylactic strategies to combat G. duodenalis infection in both humans and animals. |
| first_indexed | 2024-12-22T22:07:54Z |
| format | Article |
| id | doaj.art-5fd421bd2adf48d09b40521d379b9d1b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-22T22:07:54Z |
| publishDate | 2018-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5fd421bd2adf48d09b40521d379b9d1b2022-12-21T18:10:56ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-02-01910.3389/fmicb.2018.00089290683Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli?Thibault Allain0Thibault Allain1Soraya Chaouch2Myriam Thomas3Myriam Thomas4Marie-Agnès Travers5Isabelle Valle6Isabelle Valle7Philippe Langella8Philippe Grellier9Bruno Polack10Bruno Polack11Isabelle Florent12Luis G. Bermúdez-Humarán13INRA, Commensal and Probiotics-Host Interactions Laboratory, Micalis Institute, AgroParisTech, Paris, FranceUMR 7245, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, FranceUMR 7245, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, FranceINRA, Ecole Nationale Vétérinaire d’Alfort, BIPAR, ENVA, ANSES, UMR, Université Paris-Est, Champs-sur-Marne, FranceINRA, Laboratoire de Santé Animale, BIPAR, ENVA, ANSES, UMR, Maisons-Alfort, FranceUMR 7245, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, FranceINRA, Ecole Nationale Vétérinaire d’Alfort, BIPAR, ENVA, ANSES, UMR, Université Paris-Est, Champs-sur-Marne, FranceINRA, Laboratoire de Santé Animale, BIPAR, ENVA, ANSES, UMR, Maisons-Alfort, FranceINRA, Commensal and Probiotics-Host Interactions Laboratory, Micalis Institute, AgroParisTech, Paris, FranceUMR 7245, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, FranceINRA, Ecole Nationale Vétérinaire d’Alfort, BIPAR, ENVA, ANSES, UMR, Université Paris-Est, Champs-sur-Marne, FranceINRA, Laboratoire de Santé Animale, BIPAR, ENVA, ANSES, UMR, Maisons-Alfort, FranceUMR 7245, Muséum National d’Histoire Naturelle, Centre National de la Recherche Scientifique, Sorbonne Universités, Paris, FranceINRA, Commensal and Probiotics-Host Interactions Laboratory, Micalis Institute, AgroParisTech, Paris, FranceGiardia duodenalis is a protozoan parasite responsible for giardiasis, a disease characterized by intestinal malabsorption, diarrhea and abdominal pain in a large number of mammal species. Giardiasis is one of the most common intestinal parasitic diseases in the world and thus a high veterinary, and public health concern. It is well-established that some probiotic bacteria may confer protection against this parasite in vitro and in vivo and we recently documented the implication of bile-salt hydrolase (BSH)-like activities from strain La1 of Lactobacillus johnsonii as mediators of these effects in vitro. We showed that these activities were able to generate deconjugated bile salts that were toxic to the parasite. In the present study, a wide collection of lactobacilli strains from different ecological origins was screened to assay their anti-giardial effects. Our results revealed that the anti-parasitic effects of some of the strains tested were well-correlated with the expression of BSH-like activities. The two most active strains in vitro, La1 and Lactobacillus gasseri CNCM I-4884, were then tested for their capacity to influence G. duodenalis infection in a suckling mice model. Strikingly, only L. gasseri CNCM I-4884 strain was able to significantly antagonize parasite growth with a dramatic reduction of the trophozoites load in the small intestine. Moreover, this strain also significantly reduced the fecal excretion of Giardia cysts after 5 days of treatment, which could contribute to blocking the transmission of the parasite, in contrast of La1 where no effect was observed. This study represents a step toward the development of new prophylactic strategies to combat G. duodenalis infection in both humans and animals.http://journal.frontiersin.org/article/10.3389/fmicb.2018.00089/fullGiardia duodenalislactobacilliLactobacillus johnsoniiLactobacillus gasseriprobioticsbile salt hydrolases |
| spellingShingle | Thibault Allain Thibault Allain Soraya Chaouch Myriam Thomas Myriam Thomas Marie-Agnès Travers Isabelle Valle Isabelle Valle Philippe Langella Philippe Grellier Bruno Polack Bruno Polack Isabelle Florent Luis G. Bermúdez-Humarán Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? Frontiers in Microbiology Giardia duodenalis lactobacilli Lactobacillus johnsonii Lactobacillus gasseri probiotics bile salt hydrolases |
| title | Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? |
| title_full | Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? |
| title_fullStr | Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? |
| title_full_unstemmed | Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? |
| title_short | Bile Salt Hydrolase Activities: A Novel Target to Screen Anti-Giardia Lactobacilli? |
| title_sort | bile salt hydrolase activities a novel target to screen anti giardia lactobacilli |
| topic | Giardia duodenalis lactobacilli Lactobacillus johnsonii Lactobacillus gasseri probiotics bile salt hydrolases |
| url | http://journal.frontiersin.org/article/10.3389/fmicb.2018.00089/full |
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