<i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 Cells
Periodontitis, a disease caused by inflammation of oral bacteria, contributes to the loss of alveolar bone and destruction of connective tissues. <i>Porphyromonas gingivalis</i>, a Gram-negative bacterium, is known to possess important pathogenic factors for periodontal disease. In this...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/45/1/36 |
| _version_ | 1797444162217312256 |
|---|---|
| author | Hae-Jin Lee So-Jung Lee Sung-Kwon Lee Bong-Keun Choi Dong-Ryung Lee |
| author_facet | Hae-Jin Lee So-Jung Lee Sung-Kwon Lee Bong-Keun Choi Dong-Ryung Lee |
| author_sort | Hae-Jin Lee |
| collection | DOAJ |
| description | Periodontitis, a disease caused by inflammation of oral bacteria, contributes to the loss of alveolar bone and destruction of connective tissues. <i>Porphyromonas gingivalis</i>, a Gram-negative bacterium, is known to possess important pathogenic factors for periodontal disease. In this study, we investigated the anti-periodontitis effects of <i>Magnolia kobus</i> extract (MKE) and magnolin as a component of <i>Magnolia kobus</i> (MK) in murine macrophage RAW 264.7 cells stimulated with <i>Porphyromonas gingivalis</i> lipopolysaccharide (LPS). Effects of MKE and magnolin on the mechanism of RAW 264.7 cellular inflammation were determined by analyzing nitric oxide (NO) production and Western blot protein expression (<i>n</i> = 3). MKE/magnolin inhibited NO production without affecting cell survival. MKE/magnolin treatment inhibited LPS-induced pro-inflammatory cytokines, expression levels of matrix metalloproteinases (MMPs such as MMP-1, 3, 8, 9, and 13), and protein levels of inflammatory mediators (such as TNF-α, IL-1β, and mPGES-1). MKE/magnolin also suppressed NF-κB activation by inhibiting the TLR4 signaling pathway. These findings suggest that MKE has a therapeutic effect on inflammatory periodontal disease caused by oral bacterium <i>P. gingivalis</i> and that magnolin is a major functional component in the anti-inflammatory effect of MKE. |
| first_indexed | 2024-03-09T13:07:35Z |
| format | Article |
| id | doaj.art-5fd582abc0544eabbda3a2a37e1ab6b5 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T13:07:35Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-5fd582abc0544eabbda3a2a37e1ab6b52023-11-30T21:45:27ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-01-0145153855410.3390/cimb45010036<i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 CellsHae-Jin Lee0So-Jung Lee1Sung-Kwon Lee2Bong-Keun Choi3Dong-Ryung Lee4Research Institute, NUON Co., Ltd., Seongnam 13201, Republic of KoreaResearch Institute, NUON Co., Ltd., Seongnam 13201, Republic of KoreaResearch Institute, NUON Co., Ltd., Seongnam 13201, Republic of KoreaResearch Institute, NUON Co., Ltd., Seongnam 13201, Republic of KoreaResearch Institute, NUON Co., Ltd., Seongnam 13201, Republic of KoreaPeriodontitis, a disease caused by inflammation of oral bacteria, contributes to the loss of alveolar bone and destruction of connective tissues. <i>Porphyromonas gingivalis</i>, a Gram-negative bacterium, is known to possess important pathogenic factors for periodontal disease. In this study, we investigated the anti-periodontitis effects of <i>Magnolia kobus</i> extract (MKE) and magnolin as a component of <i>Magnolia kobus</i> (MK) in murine macrophage RAW 264.7 cells stimulated with <i>Porphyromonas gingivalis</i> lipopolysaccharide (LPS). Effects of MKE and magnolin on the mechanism of RAW 264.7 cellular inflammation were determined by analyzing nitric oxide (NO) production and Western blot protein expression (<i>n</i> = 3). MKE/magnolin inhibited NO production without affecting cell survival. MKE/magnolin treatment inhibited LPS-induced pro-inflammatory cytokines, expression levels of matrix metalloproteinases (MMPs such as MMP-1, 3, 8, 9, and 13), and protein levels of inflammatory mediators (such as TNF-α, IL-1β, and mPGES-1). MKE/magnolin also suppressed NF-κB activation by inhibiting the TLR4 signaling pathway. These findings suggest that MKE has a therapeutic effect on inflammatory periodontal disease caused by oral bacterium <i>P. gingivalis</i> and that magnolin is a major functional component in the anti-inflammatory effect of MKE.https://www.mdpi.com/1467-3045/45/1/36<i>Magnolia kobus</i>magnolinRAW 264.7periodontitis<i>Porphyromonas gingivalis</i>inflammation |
| spellingShingle | Hae-Jin Lee So-Jung Lee Sung-Kwon Lee Bong-Keun Choi Dong-Ryung Lee <i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 Cells Current Issues in Molecular Biology <i>Magnolia kobus</i> magnolin RAW 264.7 periodontitis <i>Porphyromonas gingivalis</i> inflammation |
| title | <i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 Cells |
| title_full | <i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 Cells |
| title_fullStr | <i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 Cells |
| title_full_unstemmed | <i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 Cells |
| title_short | <i>Magnolia kobus</i> Extract Inhibits Periodontitis-Inducing Mediators in <i>Porphyromonas gingivalis</i> Lipopolysaccharide-Activated RAW 264.7 Cells |
| title_sort | i magnolia kobus i extract inhibits periodontitis inducing mediators in i porphyromonas gingivalis i lipopolysaccharide activated raw 264 7 cells |
| topic | <i>Magnolia kobus</i> magnolin RAW 264.7 periodontitis <i>Porphyromonas gingivalis</i> inflammation |
| url | https://www.mdpi.com/1467-3045/45/1/36 |
| work_keys_str_mv | AT haejinlee imagnoliakobusiextractinhibitsperiodontitisinducingmediatorsiniporphyromonasgingivalisilipopolysaccharideactivatedraw2647cells AT sojunglee imagnoliakobusiextractinhibitsperiodontitisinducingmediatorsiniporphyromonasgingivalisilipopolysaccharideactivatedraw2647cells AT sungkwonlee imagnoliakobusiextractinhibitsperiodontitisinducingmediatorsiniporphyromonasgingivalisilipopolysaccharideactivatedraw2647cells AT bongkeunchoi imagnoliakobusiextractinhibitsperiodontitisinducingmediatorsiniporphyromonasgingivalisilipopolysaccharideactivatedraw2647cells AT dongryunglee imagnoliakobusiextractinhibitsperiodontitisinducingmediatorsiniporphyromonasgingivalisilipopolysaccharideactivatedraw2647cells |