2, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicity
2, 5-dichloro-1, 4-benuinone (2, 5-DCBQ) is an emerging disinfection by-product belonging to the class of halobenzoquinones (HBQs). However, there is limited evidence regarding the neurotoxic effects of 2, 5-DCBQ. To better understand the toxicological mechanisms of aquatic organisms, zebrafish embr...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651322008478 |
| _version_ | 1798032284410970112 |
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| author | Yuanyao Chen Lin Xiao Guangyu Gao Liting He Kai Zhao Xuejun Shang Chunyan Liu |
| author_facet | Yuanyao Chen Lin Xiao Guangyu Gao Liting He Kai Zhao Xuejun Shang Chunyan Liu |
| author_sort | Yuanyao Chen |
| collection | DOAJ |
| description | 2, 5-dichloro-1, 4-benuinone (2, 5-DCBQ) is an emerging disinfection by-product belonging to the class of halobenzoquinones (HBQs). However, there is limited evidence regarding the neurotoxic effects of 2, 5-DCBQ. To better understand the toxicological mechanisms of aquatic organisms, zebrafish embryos were exposed to 0.2 mg/L, 0.4 mg/L, and 0.6 mg/L of 2, 5-DCBQ from 4 h post-fertilization (hpf) to 120 hpf. Developmental defects, such as reduced body length, decreased heart rate, decreased pigmentation, and abnormal motor axon structure was observed. In particular, the locomotor activity of zebrafish larvae reduced with exposure to increasing 2, 5-DCBQ concentrations, and this effect was more pronounced under dark stimulation. The results indicated that the genes associated with neuronal development (gfap, mbp, syn2a, elavl3, ache, and a1-tubulin) were significantly downregulated after treatment with 2, 5-DCBQ. Furthermore, the KEGG result showed the neuroactive ligand-receptor interaction and apoptosis pathways were visibly disrupted, and we found acetylcholinesterase activity was also affected. In summary, the disinfection by-product, 2, 5-DCBQ, exhibits neurodevelopmental toxicity in zebrafish embryos, providing novel evidence for comprehensive analyses of its toxicity. |
| first_indexed | 2024-04-11T20:11:41Z |
| format | Article |
| id | doaj.art-5fd81370bfe640abbbeabcc3365d2d8d |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-11T20:11:41Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-5fd81370bfe640abbbeabcc3365d2d8d2022-12-22T04:05:06ZengElsevierEcotoxicology and Environmental Safety0147-65132022-09-012431140072, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicityYuanyao Chen0Lin Xiao1Guangyu Gao2Liting He3Kai Zhao4Xuejun Shang5Chunyan Liu6Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430030, PR ChinaInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430030, PR ChinaTongji Medical College, Huazhong University of Science and Technology, Hubei 430030, PR ChinaThe Second People’s Hospital of Guiyang, PR ChinaInstitute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430030, PR ChinaDepartment of Andrology, Jinling Hospital, School of Medicine, Nanjing University, Nanjing 210002, PR China; Corresponding authors.Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Hubei 430030, PR China; Corresponding authors.2, 5-dichloro-1, 4-benuinone (2, 5-DCBQ) is an emerging disinfection by-product belonging to the class of halobenzoquinones (HBQs). However, there is limited evidence regarding the neurotoxic effects of 2, 5-DCBQ. To better understand the toxicological mechanisms of aquatic organisms, zebrafish embryos were exposed to 0.2 mg/L, 0.4 mg/L, and 0.6 mg/L of 2, 5-DCBQ from 4 h post-fertilization (hpf) to 120 hpf. Developmental defects, such as reduced body length, decreased heart rate, decreased pigmentation, and abnormal motor axon structure was observed. In particular, the locomotor activity of zebrafish larvae reduced with exposure to increasing 2, 5-DCBQ concentrations, and this effect was more pronounced under dark stimulation. The results indicated that the genes associated with neuronal development (gfap, mbp, syn2a, elavl3, ache, and a1-tubulin) were significantly downregulated after treatment with 2, 5-DCBQ. Furthermore, the KEGG result showed the neuroactive ligand-receptor interaction and apoptosis pathways were visibly disrupted, and we found acetylcholinesterase activity was also affected. In summary, the disinfection by-product, 2, 5-DCBQ, exhibits neurodevelopmental toxicity in zebrafish embryos, providing novel evidence for comprehensive analyses of its toxicity.http://www.sciencedirect.com/science/article/pii/S0147651322008478Developmental malformationNeuroactive ligand-receptorNeuronal developmentLocomotor activityDichlorobenzoquinone |
| spellingShingle | Yuanyao Chen Lin Xiao Guangyu Gao Liting He Kai Zhao Xuejun Shang Chunyan Liu 2, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicity Ecotoxicology and Environmental Safety Developmental malformation Neuroactive ligand-receptor Neuronal development Locomotor activity Dichlorobenzoquinone |
| title | 2, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicity |
| title_full | 2, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicity |
| title_fullStr | 2, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicity |
| title_full_unstemmed | 2, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicity |
| title_short | 2, 5-dichloro-1, 4-benuinone exposure to zebrafish embryos/larvae causes neurodevelopmental toxicity |
| title_sort | 2 5 dichloro 1 4 benuinone exposure to zebrafish embryos larvae causes neurodevelopmental toxicity |
| topic | Developmental malformation Neuroactive ligand-receptor Neuronal development Locomotor activity Dichlorobenzoquinone |
| url | http://www.sciencedirect.com/science/article/pii/S0147651322008478 |
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