Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae
Summary: Replicative lifespan, a measure of the number of times that a yeast cell can divide before senescence, is one model for aging. Here, we provide a protocol for enrichment of yeast as a function of replicative age using a miniature chemostat aging device (mCAD). This protocol allows for isola...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722004798 |
| _version_ | 1818017832029388800 |
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| author | Emily J. Yang Liza A. Pon |
| author_facet | Emily J. Yang Liza A. Pon |
| author_sort | Emily J. Yang |
| collection | DOAJ |
| description | Summary: Replicative lifespan, a measure of the number of times that a yeast cell can divide before senescence, is one model for aging. Here, we provide a protocol for enrichment of yeast as a function of replicative age using a miniature chemostat aging device (mCAD). This protocol allows for isolation of quantities of cells that are sufficient for biochemical or genomic analysis. We also describe an approach to assess bud site selection, a marker for cell polarity, during the aging process.For complete details on the use and execution of this protocol, please refer to Yang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-14T07:32:01Z |
| format | Article |
| id | doaj.art-5fd9693ad97848d6bb9e53fba8b8e941 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-14T07:32:01Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-5fd9693ad97848d6bb9e53fba8b8e9412022-12-22T02:05:50ZengElsevierSTAR Protocols2666-16672022-09-0133101599Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiaeEmily J. Yang0Liza A. Pon1Department of Pathology and Cell Biology, Columbia University, New York, NY, USA; Corresponding authorDepartment of Pathology and Cell Biology, Columbia University, New York, NY, USA; Corresponding authorSummary: Replicative lifespan, a measure of the number of times that a yeast cell can divide before senescence, is one model for aging. Here, we provide a protocol for enrichment of yeast as a function of replicative age using a miniature chemostat aging device (mCAD). This protocol allows for isolation of quantities of cells that are sufficient for biochemical or genomic analysis. We also describe an approach to assess bud site selection, a marker for cell polarity, during the aging process.For complete details on the use and execution of this protocol, please refer to Yang et al. (2022). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722004798Cell BiologyCell isolationCell-based AssaysMicroscopyModel Organisms |
| spellingShingle | Emily J. Yang Liza A. Pon Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae STAR Protocols Cell Biology Cell isolation Cell-based Assays Microscopy Model Organisms |
| title | Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae |
| title_full | Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae |
| title_fullStr | Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae |
| title_full_unstemmed | Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae |
| title_short | Enrichment of aging yeast cells and budding polarity assay in Saccharomyces cerevisiae |
| title_sort | enrichment of aging yeast cells and budding polarity assay in saccharomyces cerevisiae |
| topic | Cell Biology Cell isolation Cell-based Assays Microscopy Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722004798 |
| work_keys_str_mv | AT emilyjyang enrichmentofagingyeastcellsandbuddingpolarityassayinsaccharomycescerevisiae AT lizaapon enrichmentofagingyeastcellsandbuddingpolarityassayinsaccharomycescerevisiae |