Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve Cells
Abstract Poly(l‐lactic acid) (PLLA) films have excellent piezoelectric properties, but the strong hydrophobicity of the surface makes it difficult for cells to attach there. PLLA nanofibers have good biocompatibility, but the weak piezoelectric coefficient limits the ability of the nanofibers to sti...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley-VCH
2023-06-01
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| Series: | Advanced Materials Interfaces |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/admi.202202474 |
| _version_ | 1827812200303034368 |
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| author | Fengying Jiang Yizhu Shan Junyuan Tian Lingling Xu Chaohai Li Fang Yu Xi Cui Chengwei Wang Zhou Li Kailiang Ren |
| author_facet | Fengying Jiang Yizhu Shan Junyuan Tian Lingling Xu Chaohai Li Fang Yu Xi Cui Chengwei Wang Zhou Li Kailiang Ren |
| author_sort | Fengying Jiang |
| collection | DOAJ |
| description | Abstract Poly(l‐lactic acid) (PLLA) films have excellent piezoelectric properties, but the strong hydrophobicity of the surface makes it difficult for cells to attach there. PLLA nanofibers have good biocompatibility, but the weak piezoelectric coefficient limits the ability of the nanofibers to stimulate cell growth. Therefore, the PLLA piezoelectric film is combined with the PLLA nanofibers to make a multilayer film. In our tests, the piezoelectric output of the multilayer film ≈260 mV. In the biological experiments section, without piezoelectric stimulation, the cell length on the nanofibers is approximately twice that of the cells in the blank control group. The length of cells cultured on piezoelectric‐based stimulated nanofibers is more than twice that of cells cultured on nanofibers without piezoelectric stimulation. Therefore, it is confirmed that under the dual action of nanofiber guidance and piezoelectric stimulation, the growth rate of cells is four times faster than that in ordinary Petri dishes. Intracellular calcium imaging experiments confirmed that the concentration of Ca2+ in electrically stimulated cells is approximately twice that of ordinary cells. It is also confirmed that piezoelectric materials can complete electrical stimulation of cells in indirect contact with cells. |
| first_indexed | 2024-03-11T23:15:31Z |
| format | Article |
| id | doaj.art-5fdbf57a48bd4f3b9704b9c51282e0b6 |
| institution | Directory Open Access Journal |
| issn | 2196-7350 |
| language | English |
| last_indexed | 2024-03-11T23:15:31Z |
| publishDate | 2023-06-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Advanced Materials Interfaces |
| spelling | doaj.art-5fdbf57a48bd4f3b9704b9c51282e0b62023-09-21T03:12:59ZengWiley-VCHAdvanced Materials Interfaces2196-73502023-06-011017n/an/a10.1002/admi.202202474Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve CellsFengying Jiang0Yizhu Shan1Junyuan Tian2Lingling Xu3Chaohai Li4Fang Yu5Xi Cui6Chengwei Wang7Zhou Li8Kailiang Ren9Center on Nanoenergy Research School of Physical Science and Technology Guangxi University Nanning 530004 P. R. ChinaBeijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing 100083 P. R. ChinaBeijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing 100083 P. R. ChinaBeijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing 100083 P. R. ChinaCenter on Nanoenergy Research School of Physical Science and Technology Guangxi University Nanning 530004 P. R. ChinaCenter on Nanoenergy Research School of Physical Science and Technology Guangxi University Nanning 530004 P. R. ChinaBeijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing 100083 P. R. ChinaBeijing Institute of Nanoenergy and Nanosystems Chinese Academy of Sciences Beijing 100083 P. R. ChinaCenter on Nanoenergy Research School of Physical Science and Technology Guangxi University Nanning 530004 P. R. ChinaCenter on Nanoenergy Research School of Physical Science and Technology Guangxi University Nanning 530004 P. R. ChinaAbstract Poly(l‐lactic acid) (PLLA) films have excellent piezoelectric properties, but the strong hydrophobicity of the surface makes it difficult for cells to attach there. PLLA nanofibers have good biocompatibility, but the weak piezoelectric coefficient limits the ability of the nanofibers to stimulate cell growth. Therefore, the PLLA piezoelectric film is combined with the PLLA nanofibers to make a multilayer film. In our tests, the piezoelectric output of the multilayer film ≈260 mV. In the biological experiments section, without piezoelectric stimulation, the cell length on the nanofibers is approximately twice that of the cells in the blank control group. The length of cells cultured on piezoelectric‐based stimulated nanofibers is more than twice that of cells cultured on nanofibers without piezoelectric stimulation. Therefore, it is confirmed that under the dual action of nanofiber guidance and piezoelectric stimulation, the growth rate of cells is four times faster than that in ordinary Petri dishes. Intracellular calcium imaging experiments confirmed that the concentration of Ca2+ in electrically stimulated cells is approximately twice that of ordinary cells. It is also confirmed that piezoelectric materials can complete electrical stimulation of cells in indirect contact with cells.https://doi.org/10.1002/admi.202202474cell growthmultilayer filmnanofiberspiezoelectric filmPLLA |
| spellingShingle | Fengying Jiang Yizhu Shan Junyuan Tian Lingling Xu Chaohai Li Fang Yu Xi Cui Chengwei Wang Zhou Li Kailiang Ren Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve Cells Advanced Materials Interfaces cell growth multilayer film nanofibers piezoelectric film PLLA |
| title | Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve Cells |
| title_full | Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve Cells |
| title_fullStr | Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve Cells |
| title_full_unstemmed | Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve Cells |
| title_short | Poly(l‐Lactic Acid) Nanofiber‐Based Multilayer Film for the Electrical Stimulation of Nerve Cells |
| title_sort | poly l lactic acid nanofiber based multilayer film for the electrical stimulation of nerve cells |
| topic | cell growth multilayer film nanofibers piezoelectric film PLLA |
| url | https://doi.org/10.1002/admi.202202474 |
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