Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation
Architecture and composition of Scaffolds are influential factors in the regeneration of defects. Herein, synthesised iron oxide (magnetite) nanoparticles (MNPs) by co‐precipitation technique were evenly distributed in polylactic‐co‐glycolic acid (PLGA)–gelatine Scaffolds. Hybrid structures were fab...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-10-01
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| Series: | IET Nanobiotechnology |
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| Online Access: | https://doi.org/10.1049/iet-nbt.2018.5305 |
| _version_ | 1826820751407185920 |
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| author | Farnaz Ghorbani Ali Zamanian Alireza Shams Atefeh Shamoosi Amir Aidun |
| author_facet | Farnaz Ghorbani Ali Zamanian Alireza Shams Atefeh Shamoosi Amir Aidun |
| author_sort | Farnaz Ghorbani |
| collection | DOAJ |
| description | Architecture and composition of Scaffolds are influential factors in the regeneration of defects. Herein, synthesised iron oxide (magnetite) nanoparticles (MNPs) by co‐precipitation technique were evenly distributed in polylactic‐co‐glycolic acid (PLGA)–gelatine Scaffolds. Hybrid structures were fabricated by freeze‐casting method to the creation of a matrix with tunable pores. The synthesised MNPs were characterised by transmission electron microscopy, Fourier transform infrared spectroscopy, X‐ray diffraction spectroscopy, and vibrating sample magnetometer analysis. Scanning electron microscopy micrographs of porous Scaffolds confirmed the formation of unidirectional microstructure, so that pore size measurement indicated the orientation of pores in the direction of solvent solidification. The addition of MNPs to the PLGA–gelatine Scaffolds had no particular effect on the morphology of the pores, but reduced slightly pore size distribution. The MNPs contained constructs demonstrated increased mechanical strength, but a reduced absorption capacity and biodegradation ratio. Stability of the MNPs and lack of iron release was the point of strength in this investigation and were determined by atomic absorption spectroscopy. The evolution of rat bone marrow mesenchymal stem cells performance on the hybrid structure under a static magnetic field indicated the potential of super‐paramagnetic constructs for further pre‐clinical and clinical studies in the field of neural regeneration. |
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| id | doaj.art-5fdc3b8e318347a9977517e8e31f3d4f |
| institution | Directory Open Access Journal |
| issn | 1751-8741 1751-875X |
| language | English |
| last_indexed | 2025-02-16T06:34:51Z |
| publishDate | 2019-10-01 |
| publisher | Wiley |
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| series | IET Nanobiotechnology |
| spelling | doaj.art-5fdc3b8e318347a9977517e8e31f3d4f2025-02-03T06:45:06ZengWileyIET Nanobiotechnology1751-87411751-875X2019-10-0113886086710.1049/iet-nbt.2018.5305Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluationFarnaz Ghorbani0Ali Zamanian1Alireza Shams2Atefeh Shamoosi3Amir Aidun4Department of OrthopaedicsShanghai Pudong Hospital, Fudan University Pudong Medical CentreShanghaiPeople's Republic of ChinaDepartment of Nanotechnology and Advanced MaterialsMaterials and Energy Research CenterKarajIranDepartment of AnatomySchool of Medicine, Alborz University of Medical SciencesKarajIranDepartment of AnatomySchool of Medicine, Alborz University of Medical SciencesKarajIranNational Cell Bank of IranPasteur Institute of IranTehranIranArchitecture and composition of Scaffolds are influential factors in the regeneration of defects. Herein, synthesised iron oxide (magnetite) nanoparticles (MNPs) by co‐precipitation technique were evenly distributed in polylactic‐co‐glycolic acid (PLGA)–gelatine Scaffolds. Hybrid structures were fabricated by freeze‐casting method to the creation of a matrix with tunable pores. The synthesised MNPs were characterised by transmission electron microscopy, Fourier transform infrared spectroscopy, X‐ray diffraction spectroscopy, and vibrating sample magnetometer analysis. Scanning electron microscopy micrographs of porous Scaffolds confirmed the formation of unidirectional microstructure, so that pore size measurement indicated the orientation of pores in the direction of solvent solidification. The addition of MNPs to the PLGA–gelatine Scaffolds had no particular effect on the morphology of the pores, but reduced slightly pore size distribution. The MNPs contained constructs demonstrated increased mechanical strength, but a reduced absorption capacity and biodegradation ratio. Stability of the MNPs and lack of iron release was the point of strength in this investigation and were determined by atomic absorption spectroscopy. The evolution of rat bone marrow mesenchymal stem cells performance on the hybrid structure under a static magnetic field indicated the potential of super‐paramagnetic constructs for further pre‐clinical and clinical studies in the field of neural regeneration.https://doi.org/10.1049/iet-nbt.2018.5305unidirectional microstructurepore size measurementmechanical strengthatomic absorption spectroscopyhybrid structuresuper‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds |
| spellingShingle | Farnaz Ghorbani Ali Zamanian Alireza Shams Atefeh Shamoosi Amir Aidun Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation IET Nanobiotechnology unidirectional microstructure pore size measurement mechanical strength atomic absorption spectroscopy hybrid structure super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds |
| title | Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation |
| title_full | Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation |
| title_fullStr | Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation |
| title_full_unstemmed | Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation |
| title_short | Fabrication and characterisation of super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds with the unidirectional porous structure: a physicochemical, mechanical, and in vitro evaluation |
| title_sort | fabrication and characterisation of super paramagnetic responsive plga gelatine magnetite scaffolds with the unidirectional porous structure a physicochemical mechanical and in vitro evaluation |
| topic | unidirectional microstructure pore size measurement mechanical strength atomic absorption spectroscopy hybrid structure super‐paramagnetic responsive PLGA–gelatine–magnetite scaffolds |
| url | https://doi.org/10.1049/iet-nbt.2018.5305 |
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