CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo
Abstract The CD24 cell surface receptor promotes apoptosis in developing B cells, and we recently found that it induces B cells to release plasma membrane-derived, CD24-bearing microvesicles (MVs). Here we have performed a systematic characterization of B cell MVs released from WEHI-231 B lymphoma c...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2017-08-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-017-08094-8 |
| _version_ | 1818343996701802496 |
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| author | D. Craig Ayre Ian C. Chute Andrew P. Joy David A. Barnett Andrew M. Hogan Marc P. Grüll Lourdes Peña-Castillo Andrew S. Lang Stephen M. Lewis Sherri L. Christian |
| author_facet | D. Craig Ayre Ian C. Chute Andrew P. Joy David A. Barnett Andrew M. Hogan Marc P. Grüll Lourdes Peña-Castillo Andrew S. Lang Stephen M. Lewis Sherri L. Christian |
| author_sort | D. Craig Ayre |
| collection | DOAJ |
| description | Abstract The CD24 cell surface receptor promotes apoptosis in developing B cells, and we recently found that it induces B cells to release plasma membrane-derived, CD24-bearing microvesicles (MVs). Here we have performed a systematic characterization of B cell MVs released from WEHI-231 B lymphoma cells in response to CD24 stimulation. We found that B cells constitutively release MVs of approximately 120 nm, and that CD24 induces an increase in phosphatidylserine-positive MV release. RNA cargo is predominantly comprised of 5S rRNA, regardless of stimulation; however, CD24 causes a decrease in the incorporation of protein coding transcripts. The MV proteome is enriched with mitochondrial and metabolism-related proteins after CD24 stimulation; however, these changes were variable and could not be fully validated by Western blotting. CD24-bearing MVs carry Siglec-2, CD63, IgM, and, unexpectedly, Ter119, but not Siglec-G or MHC-II despite their presence on the cell surface. CD24 stimulation also induces changes in CD63 and IgM expression on MVs that is not mirrored by the changes in cell surface expression. Overall, the composition of these MVs suggests that they may be involved in releasing mitochondrial components in response to pro-apoptotic stress with changes to the surface receptors potentially altering the cell type(s) that interact with the MVs. |
| first_indexed | 2024-12-13T16:39:28Z |
| format | Article |
| id | doaj.art-5fdc4db9bd1d46f7b03db8910ae362c7 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:39:28Z |
| publishDate | 2017-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5fdc4db9bd1d46f7b03db8910ae362c72022-12-21T23:38:19ZengNature PortfolioScientific Reports2045-23222017-08-017111610.1038/s41598-017-08094-8CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargoD. Craig Ayre0Ian C. Chute1Andrew P. Joy2David A. Barnett3Andrew M. Hogan4Marc P. Grüll5Lourdes Peña-Castillo6Andrew S. Lang7Stephen M. Lewis8Sherri L. Christian9Department of Biochemistry, Memorial University of NewfoundlandAtlantic Cancer Research InstituteAtlantic Cancer Research InstituteAtlantic Cancer Research InstituteDepartment of Biochemistry, Memorial University of NewfoundlandDepartments of Biology, Memorial University of NewfoundlandDepartments of Biology, Memorial University of NewfoundlandDepartments of Biology, Memorial University of NewfoundlandAtlantic Cancer Research InstituteDepartment of Biochemistry, Memorial University of NewfoundlandAbstract The CD24 cell surface receptor promotes apoptosis in developing B cells, and we recently found that it induces B cells to release plasma membrane-derived, CD24-bearing microvesicles (MVs). Here we have performed a systematic characterization of B cell MVs released from WEHI-231 B lymphoma cells in response to CD24 stimulation. We found that B cells constitutively release MVs of approximately 120 nm, and that CD24 induces an increase in phosphatidylserine-positive MV release. RNA cargo is predominantly comprised of 5S rRNA, regardless of stimulation; however, CD24 causes a decrease in the incorporation of protein coding transcripts. The MV proteome is enriched with mitochondrial and metabolism-related proteins after CD24 stimulation; however, these changes were variable and could not be fully validated by Western blotting. CD24-bearing MVs carry Siglec-2, CD63, IgM, and, unexpectedly, Ter119, but not Siglec-G or MHC-II despite their presence on the cell surface. CD24 stimulation also induces changes in CD63 and IgM expression on MVs that is not mirrored by the changes in cell surface expression. Overall, the composition of these MVs suggests that they may be involved in releasing mitochondrial components in response to pro-apoptotic stress with changes to the surface receptors potentially altering the cell type(s) that interact with the MVs.https://doi.org/10.1038/s41598-017-08094-8 |
| spellingShingle | D. Craig Ayre Ian C. Chute Andrew P. Joy David A. Barnett Andrew M. Hogan Marc P. Grüll Lourdes Peña-Castillo Andrew S. Lang Stephen M. Lewis Sherri L. Christian CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo Scientific Reports |
| title | CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo |
| title_full | CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo |
| title_fullStr | CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo |
| title_full_unstemmed | CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo |
| title_short | CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo |
| title_sort | cd24 induces changes to the surface receptors of b cell microvesicles with variable effects on their rna and protein cargo |
| url | https://doi.org/10.1038/s41598-017-08094-8 |
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