Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing mice
Context: Triple-negative breast cancer (TNBC) is a type of breast cancer with the highest aggressiveness and malignancy characteristics. Aims: To evaluate the possible anticancer potential of Cyperus rotundus rhizome extract (CRE) against 4T1-tumor-bearing mice. Methods: C. rotundus rhizome wa...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
GarVal Editorial Ltda.
2023-07-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol11/jppres23.1604_11.4.674.pdf |
| _version_ | 1827866551943954432 |
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| author | Wirdatun Nafisah Nadia Wahyuningsih Muhammad Faiz Maulana Shella Zahra Kumala Azmi Muhaimin Rifa’i Nashi Widodo Muhammad Sasmito Djati |
| author_facet | Wirdatun Nafisah Nadia Wahyuningsih Muhammad Faiz Maulana Shella Zahra Kumala Azmi Muhaimin Rifa’i Nashi Widodo Muhammad Sasmito Djati |
| author_sort | Wirdatun Nafisah |
| collection | DOAJ |
| description | Context: Triple-negative breast cancer (TNBC) is a type of breast cancer with the highest aggressiveness and malignancy characteristics.
Aims: To evaluate the possible anticancer potential of Cyperus rotundus rhizome extract (CRE) against 4T1-tumor-bearing mice.
Methods: C. rotundus rhizome was extracted using maceration methods with ethanol. C. rotundus extract (CRE) was then determined the total phenolic (TPC) and flavonoid content (TFC) using colorimetric analysis. The cytotoxic activity of CRE against 4T1 cells was carried out operating WST-1 assay, and the IC50 value was then used for in vivo study. The 4T1-tumor-bearing mice were treated with CRE at 72.5, 145, and 290 mg/kg body weight (BW) for two weeks or treated with cisplatin once a week for two weeks (4 mg/kg BW). The analysis of IL-2 cytokines production and the activation of CD4 and CD8 T cells was assessed using flow cytometry analysis. Histopathological analysis with hematoxylin and eosin (HE) staining was applied to determine the outcome of CRE on breast cancer tissue.
Results: CRE indicated a higher TPC (76.97 mg GAE/g) value than TFC (29.37 mg QE/g). This study demonstrated a significant reduction of IL-2 cytokines and CD4 T cell activation in treated groups than in the cancer group (p<0.05) and showed a good prognosis, further confirmed by histopathological data. The breast tissue of 4T1-tumor-bearing mice in treated groups showed apoptotic cells compared to the cancer group, which has more viable cells.
Conclusions: The high phenolic content in CRE can modulate mice’s immune systems and induce cancer cell apoptosis. |
| first_indexed | 2024-03-12T15:06:17Z |
| format | Article |
| id | doaj.art-5fdd2f477380445092521fff25a06012 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-03-12T15:06:17Z |
| publishDate | 2023-07-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-5fdd2f477380445092521fff25a060122023-08-12T15:13:50ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502023-07-0111467468210.56499/jppres23.1604_11.4.674Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing miceWirdatun Nafisah0https://orcid.org/0000-0001-9731-218XNadia Wahyuningsih1https://orcid.org/0000-0002-7718-0789Muhammad Faiz Maulana2https://orcid.org/0000-0003-2030-2092Shella Zahra Kumala Azmi3https://orcid.org/0000-0001-5526-3252Muhaimin Rifa’i4Nashi Widodo5Muhammad Sasmito Djati6Doctoral Program of Biology Department, Brawijaya University, Malang, Indonesia. Bioinformatics Research Center, Indonesian Institute of Bioinformatics (INBIO), Malang, IndonesiaUndergraduate Program of Biology Department, Brawijaya University, Malang, Indonesia.Undergraduate Program of Biology Department, Brawijaya University, Malang, Indonesia.Undergraduate Program of Biology Department, Brawijaya University, Malang, Indonesia.Department of Biology, Brawijaya University, Malang, Indonesia.Department of Biology, Brawijaya University, Malang, Indonesia.Department of Biology, Brawijaya University, Malang, Indonesia.Context: Triple-negative breast cancer (TNBC) is a type of breast cancer with the highest aggressiveness and malignancy characteristics. Aims: To evaluate the possible anticancer potential of Cyperus rotundus rhizome extract (CRE) against 4T1-tumor-bearing mice. Methods: C. rotundus rhizome was extracted using maceration methods with ethanol. C. rotundus extract (CRE) was then determined the total phenolic (TPC) and flavonoid content (TFC) using colorimetric analysis. The cytotoxic activity of CRE against 4T1 cells was carried out operating WST-1 assay, and the IC50 value was then used for in vivo study. The 4T1-tumor-bearing mice were treated with CRE at 72.5, 145, and 290 mg/kg body weight (BW) for two weeks or treated with cisplatin once a week for two weeks (4 mg/kg BW). The analysis of IL-2 cytokines production and the activation of CD4 and CD8 T cells was assessed using flow cytometry analysis. Histopathological analysis with hematoxylin and eosin (HE) staining was applied to determine the outcome of CRE on breast cancer tissue. Results: CRE indicated a higher TPC (76.97 mg GAE/g) value than TFC (29.37 mg QE/g). This study demonstrated a significant reduction of IL-2 cytokines and CD4 T cell activation in treated groups than in the cancer group (p<0.05) and showed a good prognosis, further confirmed by histopathological data. The breast tissue of 4T1-tumor-bearing mice in treated groups showed apoptotic cells compared to the cancer group, which has more viable cells. Conclusions: The high phenolic content in CRE can modulate mice’s immune systems and induce cancer cell apoptosis.https://jppres.com/jppres/pdf/vol11/jppres23.1604_11.4.674.pdfcyperus rotundusimmune systemtriple-negative breast cancer4t1 cells |
| spellingShingle | Wirdatun Nafisah Nadia Wahyuningsih Muhammad Faiz Maulana Shella Zahra Kumala Azmi Muhaimin Rifa’i Nashi Widodo Muhammad Sasmito Djati Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing mice Journal of Pharmacy & Pharmacognosy Research cyperus rotundus immune system triple-negative breast cancer 4t1 cells |
| title | Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing mice |
| title_full | Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing mice |
| title_fullStr | Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing mice |
| title_full_unstemmed | Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing mice |
| title_short | Cyperus rotundus L. rhizome extract modulates immune system and induces apoptotic in 4T1-tumor bearing mice |
| title_sort | cyperus rotundus l rhizome extract modulates immune system and induces apoptotic in 4t1 tumor bearing mice |
| topic | cyperus rotundus immune system triple-negative breast cancer 4t1 cells |
| url | https://jppres.com/jppres/pdf/vol11/jppres23.1604_11.4.674.pdf |
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