EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS
Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation of plants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effect of different disinfected agents (mercuric chlorite and hypochlorite), growth regul...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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University of Life Sciences in Lublin - Publishing House
2020-08-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
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| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2218 |
| _version_ | 1827587377433935872 |
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| author | Tour Jan Ikram Ullah Bilal Muhammad _ Tariq Ali Mansoor Zaheer Ullah Muhammad Asif Nawaz |
| author_facet | Tour Jan Ikram Ullah Bilal Muhammad _ Tariq Ali Mansoor Zaheer Ullah Muhammad Asif Nawaz |
| author_sort | Tour Jan |
| collection | DOAJ |
| description |
Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation of
plants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effect
of different disinfected agents (mercuric chlorite and hypochlorite), growth regulators, their concentrations
and combinations, Agar, pH, ammonium nitrate (NH4NO3) and number of subcultures. Mercuric chlorite at
0.07% and exposing time (9–10 min) was appropriate for hygienic culture. The shoots induced by Benzyladnine
(BA) alone or in combination with α-Naphthaleneacetic acid (NAA) exhibited maximum multiplication
with symptoms of hyperhydricity than those induced by Kinetin alone or in combination with NAA. Hyperhydricity
was also reduced by increasing the concentration of agar, pH and elimination of NH4NO3 from the
macroelements of Murashig and Skoog (MS) medium. Repeated subcultures affected both multiplication and
hyperhydricity. The multiplication of shoots increased from parental culture up to 5th subculture and thereafter
declined in 6th subculture. Although shoot hyperhydricity were observed from 1st subculture (19%) and
then increased up to 85% in 6th subculture. This increased in hyperhydricity could be due to the remaining
influence of hormones. In shoots of 5th subculture the content of chlorophyll (dark green) were higher than
shoots of 6th subculture.
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| first_indexed | 2024-03-09T00:16:05Z |
| format | Article |
| id | doaj.art-5fdea0d6c4464323b946c0c3ec6dc10d |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:16:05Z |
| publishDate | 2020-08-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-5fdea0d6c4464323b946c0c3ec6dc10d2023-12-12T07:51:33ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052020-08-0119410.24326/asphc.2020.4.4EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTSTour Jan0Ikram Ullah1Bilal Muhammad2_ Tariq3Ali Mansoor4Zaheer Ullah5Muhammad Asif Nawaz6Plant Tissue Culture Laboratory Department of Botany, University of Malakand, Dir Lower, KP, PakistanPlant Tissue Culture Laboratory Department of Botany, University of Malakand, Dir Lower, KP, PakistanPlant Tissue Culture Laboratory Department of Botany, University of Malakand, Dir Lower, KP, PakistanPlant Tissue Culture Laboratory Department of Botany, University of Malakand, Dir Lower, KP, PakistanPlant Tissue Culture Laboratory Department of Botany, University of Malakand, Dir Lower, KP, PakistanPlant Tissue Culture Laboratory Department of Botany, University of Malakand, Dir Lower, KP, PakistanDepartment of Biotechnology, SBBU, Sheringal, Dir Upper, KP, Pakistan Hyperhydricity is a frequently problem in plants during in vitro culture and affected micropropagation of plants. To develop an efficient in vitro regenerated system without hyperdydricity, we demonstrated the effect of different disinfected agents (mercuric chlorite and hypochlorite), growth regulators, their concentrations and combinations, Agar, pH, ammonium nitrate (NH4NO3) and number of subcultures. Mercuric chlorite at 0.07% and exposing time (9–10 min) was appropriate for hygienic culture. The shoots induced by Benzyladnine (BA) alone or in combination with α-Naphthaleneacetic acid (NAA) exhibited maximum multiplication with symptoms of hyperhydricity than those induced by Kinetin alone or in combination with NAA. Hyperhydricity was also reduced by increasing the concentration of agar, pH and elimination of NH4NO3 from the macroelements of Murashig and Skoog (MS) medium. Repeated subcultures affected both multiplication and hyperhydricity. The multiplication of shoots increased from parental culture up to 5th subculture and thereafter declined in 6th subculture. Although shoot hyperhydricity were observed from 1st subculture (19%) and then increased up to 85% in 6th subculture. This increased in hyperhydricity could be due to the remaining influence of hormones. In shoots of 5th subculture the content of chlorophyll (dark green) were higher than shoots of 6th subculture. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2218agarAmarinthus viridisHgCl2hyperhydricitypHsubcultures |
| spellingShingle | Tour Jan Ikram Ullah Bilal Muhammad _ Tariq Ali Mansoor Zaheer Ullah Muhammad Asif Nawaz EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS Acta Scientiarum Polonorum: Hortorum Cultus agar Amarinthus viridis HgCl2 hyperhydricity pH subcultures |
| title | EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS |
| title_full | EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS |
| title_fullStr | EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS |
| title_full_unstemmed | EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS |
| title_short | EFFICIENT in vitro PROPAGATION OF Amaranthus viridis L. USING NODE EXPLANTS |
| title_sort | efficient in vitro propagation of amaranthus viridis l using node explants |
| topic | agar Amarinthus viridis HgCl2 hyperhydricity pH subcultures |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/2218 |
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