Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate
Abstract Background The accumulation of fast-growing polyethylene terephthalate (PET) wastes has posed numerous threats to the environments and human health. Enzymatic degradation of PET is a promising approach for PET waste treatment. Currently, the efficiency of various PET biodegradation systems...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-12-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40643-023-00711-x |
| _version_ | 1827590769557372928 |
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| author | Jiayu Hu Yijun Chen |
| author_facet | Jiayu Hu Yijun Chen |
| author_sort | Jiayu Hu |
| collection | DOAJ |
| description | Abstract Background The accumulation of fast-growing polyethylene terephthalate (PET) wastes has posed numerous threats to the environments and human health. Enzymatic degradation of PET is a promising approach for PET waste treatment. Currently, the efficiency of various PET biodegradation systems requires further improvements. Results In this work, we engineered whole cell systems with co-display of strong adhesive proteins and the most active PETase for PET biodegradation in E. coli cells. Adhesive proteins of cp52k and mfp-3 and Fast-PETase were simultaneously displayed on the surfaces of E. coli cells, and the resulting cells displaying mfp-3 showed 50% increase of adhesion ability compared to those without adhesive proteins. Consequently, the degradation rate of E. coli cells co-displaying mfp-3 and Fast-PETase for amorphous PET exceeded 15% within 24 h, exhibiting fast and thorough PET degradation. Conclusions Through the engineering of co-display systems in E. coli cells, PET degradation efficiency was significantly increased compared to E. coli cells with sole display of Fast-PETase and free enzyme. This feasible E. coli co-display system could be served as a convenient tool for extending the treatment options for PET biodegradation. Graphical Abstract |
| first_indexed | 2024-03-09T01:21:27Z |
| format | Article |
| id | doaj.art-5fdff8c88bb94bcab34d5d561da3ca03 |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-03-09T01:21:27Z |
| publishDate | 2023-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-5fdff8c88bb94bcab34d5d561da3ca032023-12-10T12:05:34ZengSpringerOpenBioresources and Bioprocessing2197-43652023-12-0110111010.1186/s40643-023-00711-xConstructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalateJiayu Hu0Yijun Chen1Laboratory of Chemical Biology, School of Life Science and Technology, China Pharmaceutical UniversityLaboratory of Chemical Biology, School of Life Science and Technology, China Pharmaceutical UniversityAbstract Background The accumulation of fast-growing polyethylene terephthalate (PET) wastes has posed numerous threats to the environments and human health. Enzymatic degradation of PET is a promising approach for PET waste treatment. Currently, the efficiency of various PET biodegradation systems requires further improvements. Results In this work, we engineered whole cell systems with co-display of strong adhesive proteins and the most active PETase for PET biodegradation in E. coli cells. Adhesive proteins of cp52k and mfp-3 and Fast-PETase were simultaneously displayed on the surfaces of E. coli cells, and the resulting cells displaying mfp-3 showed 50% increase of adhesion ability compared to those without adhesive proteins. Consequently, the degradation rate of E. coli cells co-displaying mfp-3 and Fast-PETase for amorphous PET exceeded 15% within 24 h, exhibiting fast and thorough PET degradation. Conclusions Through the engineering of co-display systems in E. coli cells, PET degradation efficiency was significantly increased compared to E. coli cells with sole display of Fast-PETase and free enzyme. This feasible E. coli co-display system could be served as a convenient tool for extending the treatment options for PET biodegradation. Graphical Abstracthttps://doi.org/10.1186/s40643-023-00711-xPET biodegradationEscherichia coliSurface displayAdhesion |
| spellingShingle | Jiayu Hu Yijun Chen Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate Bioresources and Bioprocessing PET biodegradation Escherichia coli Surface display Adhesion |
| title | Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate |
| title_full | Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate |
| title_fullStr | Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate |
| title_full_unstemmed | Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate |
| title_short | Constructing Escherichia coli co-display systems for biodegradation of polyethylene terephthalate |
| title_sort | constructing escherichia coli co display systems for biodegradation of polyethylene terephthalate |
| topic | PET biodegradation Escherichia coli Surface display Adhesion |
| url | https://doi.org/10.1186/s40643-023-00711-x |
| work_keys_str_mv | AT jiayuhu constructingescherichiacolicodisplaysystemsforbiodegradationofpolyethyleneterephthalate AT yijunchen constructingescherichiacolicodisplaysystemsforbiodegradationofpolyethyleneterephthalate |