Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i>
Recently, global health professionals have been significantly challenged by the emergence of <i>Candida auris</i> and its propensity to colonize human skin, persist in the healthcare environment, and cause healthcare-associated outbreaks. Additionally, <i>C. auris</i> isolate...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-08-01
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| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/5/3/77 |
| _version_ | 1831615101770137600 |
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| author | Milena Kordalewska David S. Perlin |
| author_facet | Milena Kordalewska David S. Perlin |
| author_sort | Milena Kordalewska |
| collection | DOAJ |
| description | Recently, global health professionals have been significantly challenged by the emergence of <i>Candida auris</i> and its propensity to colonize human skin, persist in the healthcare environment, and cause healthcare-associated outbreaks. Additionally, <i>C. auris</i> isolates are often characterized by elevated minimal inhibitory concentration (MIC) values for antifungal drugs. Thus, rapid detection and accurate identification of <i>C. auris</i> together with an assessment of potential antifungal drug resistance has become essential for effective patient management, and infection prevention and control in healthcare facilities. Surprisingly, almost all of the commonly available diagnostic tools rely on recovery (growth) of yeast colonies from collected samples, which delays the diagnostic result by several days or longer. To circumvent these issues, molecular-based DNA amplification assays have been developed to identify <i>C. auris</i> DNA directly from patient samples. Moreover, allele discriminating detection probes can be used to rapidly assess validated mechanisms of echinocandin and azole resistance. |
| first_indexed | 2024-12-18T19:41:29Z |
| format | Article |
| id | doaj.art-5fe2f1f135b444c98fb04fefc2b7a540 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-12-18T19:41:29Z |
| publishDate | 2019-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-5fe2f1f135b444c98fb04fefc2b7a5402022-12-21T20:55:25ZengMDPI AGJournal of Fungi2309-608X2019-08-01537710.3390/jof5030077jof5030077Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i>Milena Kordalewska0David S. Perlin1Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ 07110, USACenter for Discovery and Innovation, Hackensack Meridian Health, Nutley, NJ 07110, USARecently, global health professionals have been significantly challenged by the emergence of <i>Candida auris</i> and its propensity to colonize human skin, persist in the healthcare environment, and cause healthcare-associated outbreaks. Additionally, <i>C. auris</i> isolates are often characterized by elevated minimal inhibitory concentration (MIC) values for antifungal drugs. Thus, rapid detection and accurate identification of <i>C. auris</i> together with an assessment of potential antifungal drug resistance has become essential for effective patient management, and infection prevention and control in healthcare facilities. Surprisingly, almost all of the commonly available diagnostic tools rely on recovery (growth) of yeast colonies from collected samples, which delays the diagnostic result by several days or longer. To circumvent these issues, molecular-based DNA amplification assays have been developed to identify <i>C. auris</i> DNA directly from patient samples. Moreover, allele discriminating detection probes can be used to rapidly assess validated mechanisms of echinocandin and azole resistance.https://www.mdpi.com/2309-608X/5/3/77<i>Candida auris</i>diagnosticsmolecular diagnosticsidentificationdetectionantifungal drug resistanceazolesechinocandins |
| spellingShingle | Milena Kordalewska David S. Perlin Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i> Journal of Fungi <i>Candida auris</i> diagnostics molecular diagnostics identification detection antifungal drug resistance azoles echinocandins |
| title | Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i> |
| title_full | Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i> |
| title_fullStr | Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i> |
| title_full_unstemmed | Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i> |
| title_short | Molecular Diagnostics in the Times of Surveillance for <i>Candida auris</i> |
| title_sort | molecular diagnostics in the times of surveillance for i candida auris i |
| topic | <i>Candida auris</i> diagnostics molecular diagnostics identification detection antifungal drug resistance azoles echinocandins |
| url | https://www.mdpi.com/2309-608X/5/3/77 |
| work_keys_str_mv | AT milenakordalewska moleculardiagnosticsinthetimesofsurveillanceforicandidaaurisi AT davidsperlin moleculardiagnosticsinthetimesofsurveillanceforicandidaaurisi |