Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells
Bladder cancer (BC) is the tenth most commonly diagnosed cancer. High recurrence, chemoresistance, and low response rate hinder the effective treatment of BC. Hence, a novel therapeutic strategy in the clinical management of BC is urgently needed. Medicarpin (MED), an isoflavone from Dalbergia odori...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2023-06-01
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| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2023-0016 |
| _version_ | 1797800838426525696 |
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| author | Chen Yuan Yin Liqi Hao Mingxuan Xu Wenkai Gao Jixian Sun Yuxin Wang Qiao Chen Shi Liang Youfeng Guo Rui Zhang Jinku Li Jinmei Zhai Qiongli Cheng Runfen Wang Jiansong Wang Haifeng Yang Zhao |
| author_facet | Chen Yuan Yin Liqi Hao Mingxuan Xu Wenkai Gao Jixian Sun Yuxin Wang Qiao Chen Shi Liang Youfeng Guo Rui Zhang Jinku Li Jinmei Zhai Qiongli Cheng Runfen Wang Jiansong Wang Haifeng Yang Zhao |
| author_sort | Chen Yuan |
| collection | DOAJ |
| description | Bladder cancer (BC) is the tenth most commonly diagnosed cancer. High recurrence, chemoresistance, and low response rate hinder the effective treatment of BC. Hence, a novel therapeutic strategy in the clinical management of BC is urgently needed. Medicarpin (MED), an isoflavone from Dalbergia odorifera, can promote bone mass gain and kill tumor cells, but its anti-BC effect remains obscure. This study reve aled that MED effectively inhibited the proliferation and arrested the cell cycle at the G1 phase of BC cell lines T24 and EJ-1 in vitro. In addition, MED could significantly suppress the tumor growth of BC cells in vivo. Mechanically, MED induced cell apoptosis by upregulating pro-apoptotic proteins BAK1, Bcl2-L-11, and caspase-3. Our data suggest that MED suppresses BC cell growth in vitro and in vivo via regulating mitochondria-mediated intrinsic apoptotic pathways, which can serve as a promising candidate for BC therapy. |
| first_indexed | 2024-03-13T04:41:20Z |
| format | Article |
| id | doaj.art-5fe34c9fd68e49f0948e7473c05ab377 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-03-13T04:41:20Z |
| publishDate | 2023-06-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-5fe34c9fd68e49f0948e7473c05ab3772023-06-19T05:54:11ZengSciendoActa Pharmaceutica1846-95582023-06-0173221122510.2478/acph-2023-0016Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cellsChen Yuan0Yin Liqi1Hao Mingxuan2Xu Wenkai3Gao Jixian4Sun Yuxin5Wang Qiao6Chen Shi7Liang Youfeng8Guo Rui9Zhang Jinku10Li Jinmei11Zhai Qiongli12Cheng Runfen13Wang Jiansong14Wang Haifeng15Yang Zhao161College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing100029, China1College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing100029, China1College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing100029, China2Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming650101, China2Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming650101, China2Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming650101, China2Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming650101, China2Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming650101, China1College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing100029, China1College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing100029, China3Department of Pathology, First Central Hospital of Baoding City, Baoding 071000, Hebei, China3Department of Pathology, First Central Hospital of Baoding City, Baoding 071000, Hebei, China5Department of Pathology, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin300060, China5Department of Pathology, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin300060, China2Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming650101, China2Department of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming650101, China1College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing100029, ChinaBladder cancer (BC) is the tenth most commonly diagnosed cancer. High recurrence, chemoresistance, and low response rate hinder the effective treatment of BC. Hence, a novel therapeutic strategy in the clinical management of BC is urgently needed. Medicarpin (MED), an isoflavone from Dalbergia odorifera, can promote bone mass gain and kill tumor cells, but its anti-BC effect remains obscure. This study reve aled that MED effectively inhibited the proliferation and arrested the cell cycle at the G1 phase of BC cell lines T24 and EJ-1 in vitro. In addition, MED could significantly suppress the tumor growth of BC cells in vivo. Mechanically, MED induced cell apoptosis by upregulating pro-apoptotic proteins BAK1, Bcl2-L-11, and caspase-3. Our data suggest that MED suppresses BC cell growth in vitro and in vivo via regulating mitochondria-mediated intrinsic apoptotic pathways, which can serve as a promising candidate for BC therapy.https://doi.org/10.2478/acph-2023-0016bladder cancermedicarpincell cyclearrestapoptosistherapy |
| spellingShingle | Chen Yuan Yin Liqi Hao Mingxuan Xu Wenkai Gao Jixian Sun Yuxin Wang Qiao Chen Shi Liang Youfeng Guo Rui Zhang Jinku Li Jinmei Zhai Qiongli Cheng Runfen Wang Jiansong Wang Haifeng Yang Zhao Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells Acta Pharmaceutica bladder cancer medicarpin cell cycle arrest apoptosis therapy |
| title | Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells |
| title_full | Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells |
| title_fullStr | Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells |
| title_full_unstemmed | Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells |
| title_short | Medicarpin induces G1 arrest and mitochondria-mediated intrinsic apoptotic pathway in bladder cancer cells |
| title_sort | medicarpin induces g1 arrest and mitochondria mediated intrinsic apoptotic pathway in bladder cancer cells |
| topic | bladder cancer medicarpin cell cycle arrest apoptosis therapy |
| url | https://doi.org/10.2478/acph-2023-0016 |
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