Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China
The plasmid-borne cfr gene, mediating multiple drug resistance (MDR), has been observed in many Gram-positive bacteria. The prevalence of cfr and its co-occurrence with additional antimicrobial resistance (AMR) determinants in Escherichia coli is an ongoing issue. Additionally, the prevalence and tr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.999778/full |
| _version_ | 1811275456864845824 |
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| author | Biao Tang Juan Ni Juan Ni Jiahui Lin Yangying Sun Hui Lin Yuehong Wu Hua Yang Min Yue Min Yue |
| author_facet | Biao Tang Juan Ni Juan Ni Jiahui Lin Yangying Sun Hui Lin Yuehong Wu Hua Yang Min Yue Min Yue |
| author_sort | Biao Tang |
| collection | DOAJ |
| description | The plasmid-borne cfr gene, mediating multiple drug resistance (MDR), has been observed in many Gram-positive bacteria. The prevalence of cfr and its co-occurrence with additional antimicrobial resistance (AMR) determinants in Escherichia coli is an ongoing issue. Additionally, the prevalence and transfer mechanism of the cfr gene remain partially investigated. Here, eight cfr-positive E. coli strains were screened using PCR from an extensive collection of E. coli (n = 2,165) strains isolated from pigs and chickens in 2021 in China, with a prevalence rate of 0.37%. All of them were MDR and resistant to florfenicol and tetracycline. These strains can transfer the cfr gene to E. coli J53 by conjugation (1.05 × 10−1 – 1.01 × 10−6). Moreover, the IncX4 plasmid p727A3-62 K-cfr (62,717 bp) harboring cfr in strain EC727A3 was confirmed using Oxford Nanopore Technology. The unknown type plasmid p737A1-27K-cfr (27,742 bp) harboring cfr in strain EC737A1 was also identified. Notably, it was verified by PCR that three of the eight E. coli strains were able to form the cfr-IS26 circular intermediate. It was 2,365 bp in length in strains EC727A3 and ECJHZ21-173, and 2,022 bp in length in EC737A1. Collectively, this study demonstrated that IS26 plays a vital role in transmitting the MDR gene cfr in E. coli via conjugation and provided updated knowledge regarding cfr in E. coli in Eastern China. |
| first_indexed | 2024-04-12T23:38:09Z |
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| id | doaj.art-5feca2f4ff0b4ac7a45bfcab7461195c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T23:38:09Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-5feca2f4ff0b4ac7a45bfcab7461195c2022-12-22T03:12:04ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.999778999778Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern ChinaBiao Tang0Juan Ni1Juan Ni2Jiahui Lin3Yangying Sun4Hui Lin5Yuehong Wu6Hua Yang7Min Yue8Min Yue9State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-product Safety and Nutrition, Academy of Agricultural Sciences, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-product Safety and Nutrition, Academy of Agricultural Sciences, Hangzhou, ChinaSchool of Food and Pharmacy, Ningbo University, Ningbo, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, ChinaSchool of Food and Pharmacy, Ningbo University, Ningbo, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-product Safety and Nutrition, Academy of Agricultural Sciences, Hangzhou, ChinaCollege of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Institute of Agro-product Safety and Nutrition, Academy of Agricultural Sciences, Hangzhou, ChinaDepartment of Veterinary Medicine, Institute of Preventive Veterinary Sciences, Zhejiang University College of Animal Sciences, Hangzhou, ChinaHainan Institute of Zhejiang University, Sanya, ChinaThe plasmid-borne cfr gene, mediating multiple drug resistance (MDR), has been observed in many Gram-positive bacteria. The prevalence of cfr and its co-occurrence with additional antimicrobial resistance (AMR) determinants in Escherichia coli is an ongoing issue. Additionally, the prevalence and transfer mechanism of the cfr gene remain partially investigated. Here, eight cfr-positive E. coli strains were screened using PCR from an extensive collection of E. coli (n = 2,165) strains isolated from pigs and chickens in 2021 in China, with a prevalence rate of 0.37%. All of them were MDR and resistant to florfenicol and tetracycline. These strains can transfer the cfr gene to E. coli J53 by conjugation (1.05 × 10−1 – 1.01 × 10−6). Moreover, the IncX4 plasmid p727A3-62 K-cfr (62,717 bp) harboring cfr in strain EC727A3 was confirmed using Oxford Nanopore Technology. The unknown type plasmid p737A1-27K-cfr (27,742 bp) harboring cfr in strain EC737A1 was also identified. Notably, it was verified by PCR that three of the eight E. coli strains were able to form the cfr-IS26 circular intermediate. It was 2,365 bp in length in strains EC727A3 and ECJHZ21-173, and 2,022 bp in length in EC737A1. Collectively, this study demonstrated that IS26 plays a vital role in transmitting the MDR gene cfr in E. coli via conjugation and provided updated knowledge regarding cfr in E. coli in Eastern China.https://www.frontiersin.org/articles/10.3389/fmicb.2022.999778/fullEscherichia coliflorfenicolcfrantimicrobial resistancecircular intermediate |
| spellingShingle | Biao Tang Juan Ni Juan Ni Jiahui Lin Yangying Sun Hui Lin Yuehong Wu Hua Yang Min Yue Min Yue Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China Frontiers in Microbiology Escherichia coli florfenicol cfr antimicrobial resistance circular intermediate |
| title | Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China |
| title_full | Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China |
| title_fullStr | Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China |
| title_full_unstemmed | Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China |
| title_short | Genomic characterization of multidrug-resistance gene cfr in Escherichia coli recovered from food animals in Eastern China |
| title_sort | genomic characterization of multidrug resistance gene cfr in escherichia coli recovered from food animals in eastern china |
| topic | Escherichia coli florfenicol cfr antimicrobial resistance circular intermediate |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.999778/full |
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