RNA 5-methylcytosine regulates YBX2-dependent liquid-liquid phase separation

RNA 5-methylcytosine regulates YBX2-dependent liquid-liquid phase separation

5-Methylcytosine (m5C) is one of the most prevalent internal modifications of messenger RNA (mRNA) in higher eukaryotes. Here we report that Y box protein 2 (YBX2) serves as a novel mammalian m5C binding protein to undergo liquid-liquid phase separation (LLPS) both in vivo and in vitro, and this YBX...

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Bibliographic Details
Main Authors: Xiuzhi Wang, Mengke Wang, Xinyuan Dai, Xiao Han, Yu Zhou, Weiyi Lai, Liyuan Zhang, Ying Yang, Yusheng Chen, Hailin Wang, Yong-Liang Zhao, Bin Shen, Yuhan Zhang, Ying Huang, Yun-Gui Yang
Format: Article
Language:English
Published: KeAi Communications Co. Ltd. 2022-01-01
Series:Fundamental Research
Subjects:
RNA modification
RNA m5C
YBX2
Phase separation
Liquid droplet
Online Access:http://www.sciencedirect.com/science/article/pii/S2667325821002600
Description
Summary:5-Methylcytosine (m5C) is one of the most prevalent internal modifications of messenger RNA (mRNA) in higher eukaryotes. Here we report that Y box protein 2 (YBX2) serves as a novel mammalian m5C binding protein to undergo liquid-liquid phase separation (LLPS) both in vivo and in vitro, and this YBX2-dependent LLPS is enhanced by m5C marked RNA. Furthermore, the crystal structure assay revealed that W100, as a distinct m5C binding site of YBX2, is critical in mediating YBX2 phase separation. Our study resolved the relationship between RNA m5C and phase separation, providing a clue for a new regulatory layer of epigenetics.
ISSN:2667-3258

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