Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber
Abstract Background Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2017-06-01
|
| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12896-017-0373-2 |
| _version_ | 1818600427252350976 |
|---|---|
| author | Yan-Jun Li Shou-Hong Zhu Xin-Yu Zhang Yong-Chang Liu Fei Xue Lan-Jie Zhao Jie Sun |
| author_facet | Yan-Jun Li Shou-Hong Zhu Xin-Yu Zhang Yong-Chang Liu Fei Xue Lan-Jie Zhao Jie Sun |
| author_sort | Yan-Jun Li |
| collection | DOAJ |
| description | Abstract Background Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. The Kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development. Results The full length of coding sequence of GhKIS13A1 was cloned using cDNA from cotton fiber for functional characterization. Expression pattern analysis showed that GhKIS13A1 maintained a lower expression level during cotton fiber development. Biochemical assay showed that GhKIS13A1 has microtubule binding activity and basal ATPase activity that can be activated significantly by the presence of microtubules. Overexpression of GhKIS13A1 in Arabidopsis reduced leaf trichomes and the percentage of three-branch trichomes, and increased two-branch and shriveled trichomes compared to wild-type. Additionally, the expression of GhKIS13A1 in the Arabidopsis Kinesin-13a-1 mutant rescued the defective trichome branching pattern of the mutant, making its overall trichome branching pattern back to normal. Conclusions Our results suggested that GhKIS13A1 is functionally compatible with AtKinesin-13A regarding their role in regulating the number and branching pattern of leaf trichomes. Given the developmental similarities between cotton fibers and Arabidopsis trichomes, it is speculated that GhKIS13A1 may also be involved in the regulation of cotton fiber development. |
| first_indexed | 2024-12-16T12:35:19Z |
| format | Article |
| id | doaj.art-5ff35e3a8cc84f1e831dac4ddea01ed3 |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-16T12:35:19Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biotechnology |
| spelling | doaj.art-5ff35e3a8cc84f1e831dac4ddea01ed32022-12-21T22:31:35ZengBMCBMC Biotechnology1472-67502017-06-011711910.1186/s12896-017-0373-2Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiberYan-Jun Li0Shou-Hong Zhu1Xin-Yu Zhang2Yong-Chang Liu3Fei Xue4Lan-Jie Zhao5Jie Sun6The Key Laboratory of Oasis Eco-agriculture, Agriculture College, Shihezi UniversityThe Key Laboratory of Oasis Eco-agriculture, Agriculture College, Shihezi UniversityThe Key Laboratory of Oasis Eco-agriculture, Agriculture College, Shihezi UniversityThe Key Laboratory of Oasis Eco-agriculture, Agriculture College, Shihezi UniversityThe Key Laboratory of Oasis Eco-agriculture, Agriculture College, Shihezi UniversityCollege of Life Sciences, Shihezi UniversityThe Key Laboratory of Oasis Eco-agriculture, Agriculture College, Shihezi UniversityAbstract Background Cotton fiber, a natural fiber widely used in the textile industry, is differentiated from single cell of ovule epidermis. A large number of genes are believed to be involved in fiber formation, but so far only a few fiber genes have been isolated and functionally characterized in this developmental process. The Kinesin13 subfamily was found to play key roles during cell division and cell elongation, and was considered to be involved in the regulation of cotton fiber development. Results The full length of coding sequence of GhKIS13A1 was cloned using cDNA from cotton fiber for functional characterization. Expression pattern analysis showed that GhKIS13A1 maintained a lower expression level during cotton fiber development. Biochemical assay showed that GhKIS13A1 has microtubule binding activity and basal ATPase activity that can be activated significantly by the presence of microtubules. Overexpression of GhKIS13A1 in Arabidopsis reduced leaf trichomes and the percentage of three-branch trichomes, and increased two-branch and shriveled trichomes compared to wild-type. Additionally, the expression of GhKIS13A1 in the Arabidopsis Kinesin-13a-1 mutant rescued the defective trichome branching pattern of the mutant, making its overall trichome branching pattern back to normal. Conclusions Our results suggested that GhKIS13A1 is functionally compatible with AtKinesin-13A regarding their role in regulating the number and branching pattern of leaf trichomes. Given the developmental similarities between cotton fibers and Arabidopsis trichomes, it is speculated that GhKIS13A1 may also be involved in the regulation of cotton fiber development.http://link.springer.com/article/10.1186/s12896-017-0373-2Kinesin13 subfamilyCotton fiberTrichomeGhKIS13A1 |
| spellingShingle | Yan-Jun Li Shou-Hong Zhu Xin-Yu Zhang Yong-Chang Liu Fei Xue Lan-Jie Zhao Jie Sun Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber BMC Biotechnology Kinesin13 subfamily Cotton fiber Trichome GhKIS13A1 |
| title | Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber |
| title_full | Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber |
| title_fullStr | Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber |
| title_full_unstemmed | Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber |
| title_short | Expression and functional analyses of a Kinesin gene GhKIS13A1 from cotton (Gossypium hirsutum) fiber |
| title_sort | expression and functional analyses of a kinesin gene ghkis13a1 from cotton gossypium hirsutum fiber |
| topic | Kinesin13 subfamily Cotton fiber Trichome GhKIS13A1 |
| url | http://link.springer.com/article/10.1186/s12896-017-0373-2 |
| work_keys_str_mv | AT yanjunli expressionandfunctionalanalysesofakinesingeneghkis13a1fromcottongossypiumhirsutumfiber AT shouhongzhu expressionandfunctionalanalysesofakinesingeneghkis13a1fromcottongossypiumhirsutumfiber AT xinyuzhang expressionandfunctionalanalysesofakinesingeneghkis13a1fromcottongossypiumhirsutumfiber AT yongchangliu expressionandfunctionalanalysesofakinesingeneghkis13a1fromcottongossypiumhirsutumfiber AT feixue expressionandfunctionalanalysesofakinesingeneghkis13a1fromcottongossypiumhirsutumfiber AT lanjiezhao expressionandfunctionalanalysesofakinesingeneghkis13a1fromcottongossypiumhirsutumfiber AT jiesun expressionandfunctionalanalysesofakinesingeneghkis13a1fromcottongossypiumhirsutumfiber |