Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions

<p>Abstract</p> <p>Background</p> <p>Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development o...

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Bibliographic Details
Main Authors: Puisieux Alain, Combaret Valerie, Bell Sandra M, Field Sarah L, Douglas Susan H, Leong Fong T, Bransfield Kieran, Toomes Carmel, Ponchel Frederique, Mighell Alan J, Robinson Philip A, Inglehearn Chris F, Isaacs John D, Markham Alex F
Format: Article
Language:English
Published: BMC 2003-10-01
Series:BMC Biotechnology
Subjects:
Real-time PCR
SYBR-green
rearrangement
amplification
deletion
Online Access:http://www.biomedcentral.com/1472-6750/3/18

Internet

http://www.biomedcentral.com/1472-6750/3/18

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