Real-time PCR based on SYBR-Green I fluorescence: An alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions
<p>Abstract</p> <p>Background</p> <p>Real-time PCR is increasingly being adopted for RNA quantification and genetic analysis. At present the most popular real-time PCR assay is based on the hybridisation of a dual-labelled probe to the PCR product, and the development o...
Main Authors: | Puisieux Alain, Combaret Valerie, Bell Sandra M, Field Sarah L, Douglas Susan H, Leong Fong T, Bransfield Kieran, Toomes Carmel, Ponchel Frederique, Mighell Alan J, Robinson Philip A, Inglehearn Chris F, Isaacs John D, Markham Alex F |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2003-10-01
|
Series: | BMC Biotechnology |
Subjects: | |
Online Access: | http://www.biomedcentral.com/1472-6750/3/18 |
Similar Items
-
Comparison of SYBR Green and TaqMan methods in quantitative real-time polymerase chain reaction analysis of four adenosine receptor subtypes
by: Mohamadhasan Tajadini, et al.
Published: (2014-01-01) -
Comparison of the sensitivity of determining soyeabean allergens by ELISA method and SYBR green I
by: Jozef Golian, et al.
Published: (2013-07-01) -
Development of a Pan-<i>Filoviridae</i> SYBR Green qPCR Assay for Biosurveillance Studies in Bats
by: Jessica Coertse, et al.
Published: (2023-04-01) -
Rapid visual detection of anisakid nematodes using recombinase polymerase amplification and SYBR Green I
by: Xiuqin Chen, et al.
Published: (2022-12-01) -
Development of a SYBR green real-time PCR method for rapid detection of sheep pox virus
by: Tian Hong, et al.
Published: (2012-11-01)