Janus kinase-1 and 3 in ankylosing spondylitis
Background/purpose: To investigate the Janus kinase-1 and 3 (JAK-1 and 3) expression in peripheral blood mononuclear cells (PBMCs) in ankylosing spondylitis (AS). Methods: The levels of JAK-1 and JAK-3 mRNA in PBMCs, CD3+ T cells and CD14+ monocytes were measured by RT-PCR in 52 AS patients and 31 h...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-01-01
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| Series: | Journal of the Formosan Medical Association |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0929664617305843 |
| _version_ | 1811310422886711296 |
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| author | Hsien-Tzung Liao Tzu-Hao Li Chun-Hsiung Chen Hung-An Chen Wei-Sheng Chen Chien-Chih Lai Chung-Tei Chou Chang-Youh Tsai |
| author_facet | Hsien-Tzung Liao Tzu-Hao Li Chun-Hsiung Chen Hung-An Chen Wei-Sheng Chen Chien-Chih Lai Chung-Tei Chou Chang-Youh Tsai |
| author_sort | Hsien-Tzung Liao |
| collection | DOAJ |
| description | Background/purpose: To investigate the Janus kinase-1 and 3 (JAK-1 and 3) expression in peripheral blood mononuclear cells (PBMCs) in ankylosing spondylitis (AS). Methods: The levels of JAK-1 and JAK-3 mRNA in PBMCs, CD3+ T cells and CD14+ monocytes were measured by RT-PCR in 52 AS patients and 31 healthy controls (HCs). The demographic features, BASDAI, BASFI, HLA-B27, ESR, CRP and serum immunoglobulin A (IgA) level were recorded and correlated with the JAK-1 & JAK-3 transcripts in patients and HCs as appropriate. Results: JAK-1 and JAK-3 expression in PB CD3+ T cells plus CD14+ monocytes was significantly higher in AS patients than in HCs (p < 0.05). There is a positive correlation between JAK-1 expression in CD3+ T cells plus CD14+ monocytes and ESR, CRP, IgA, HLA-B27, peripheral arthritis, enthesitis and uveitis (all p < 0.05), respectively. JAK-1 transcript was also increased in CD14+ monocytes from patients and correlated well with ESR and CRP as the disease deteriorated. Conversely, JAK-1 was negatively correlated to chest expansion. Area under the curve of standard receiver operating characteristic suggested that JAK-1 transcript in CD3+ T cells plus CD14+ monocytes is better to predict the higher BASDAI (>4) and BASFI (>4) than ESR or CRP in AS patients. Conclusion: In AS, JAK-1 expression in PB cells rather than ESR or CRP might be regarded as a bio-marker for monitoring disease activity and functional index in AS. These findings have also suggested that JAK-1 and JAK-3 expression may play a role in the inflammatory processes in patients with AS. Keywords: Ankylosing spondylitis, Janus kinase (JAK), Acute-phase reactant |
| first_indexed | 2024-04-13T09:58:30Z |
| format | Article |
| id | doaj.art-5ffa246d5023424da93b81b9d9361ca6 |
| institution | Directory Open Access Journal |
| issn | 0929-6646 |
| language | English |
| last_indexed | 2024-04-13T09:58:30Z |
| publishDate | 2019-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of the Formosan Medical Association |
| spelling | doaj.art-5ffa246d5023424da93b81b9d9361ca62022-12-22T02:51:17ZengElsevierJournal of the Formosan Medical Association0929-66462019-01-011181134141Janus kinase-1 and 3 in ankylosing spondylitisHsien-Tzung Liao0Tzu-Hao Li1Chun-Hsiung Chen2Hung-An Chen3Wei-Sheng Chen4Chien-Chih Lai5Chung-Tei Chou6Chang-Youh Tsai7Division of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Division of Allergy, Immunology and Rheumatology, Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanDivision of Allergy, Immunology, and Rheumatology, Department of Medicine, Chiayi Branch, Taichung Veterans General Hospital, TaiwanDivision of Allergy, Immunology, and Rheumatology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, TaiwanChi Mei Medical Center, Tainan, TaiwanDivision of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanDivision of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanDivision of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, TaiwanDivision of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan; Faculty of Medicine, National Yang-Ming University School of Medicine, Taipei, Taiwan; Corresponding author. Division of Allergy, Immunology and Rheumatology, Department of Medicine, Taipei Veterans General Hospital, 201 Shi-pai Rd Sec 2, Taipei, 112, Taiwan. Fax: + 886 2 2872 1874.Background/purpose: To investigate the Janus kinase-1 and 3 (JAK-1 and 3) expression in peripheral blood mononuclear cells (PBMCs) in ankylosing spondylitis (AS). Methods: The levels of JAK-1 and JAK-3 mRNA in PBMCs, CD3+ T cells and CD14+ monocytes were measured by RT-PCR in 52 AS patients and 31 healthy controls (HCs). The demographic features, BASDAI, BASFI, HLA-B27, ESR, CRP and serum immunoglobulin A (IgA) level were recorded and correlated with the JAK-1 & JAK-3 transcripts in patients and HCs as appropriate. Results: JAK-1 and JAK-3 expression in PB CD3+ T cells plus CD14+ monocytes was significantly higher in AS patients than in HCs (p < 0.05). There is a positive correlation between JAK-1 expression in CD3+ T cells plus CD14+ monocytes and ESR, CRP, IgA, HLA-B27, peripheral arthritis, enthesitis and uveitis (all p < 0.05), respectively. JAK-1 transcript was also increased in CD14+ monocytes from patients and correlated well with ESR and CRP as the disease deteriorated. Conversely, JAK-1 was negatively correlated to chest expansion. Area under the curve of standard receiver operating characteristic suggested that JAK-1 transcript in CD3+ T cells plus CD14+ monocytes is better to predict the higher BASDAI (>4) and BASFI (>4) than ESR or CRP in AS patients. Conclusion: In AS, JAK-1 expression in PB cells rather than ESR or CRP might be regarded as a bio-marker for monitoring disease activity and functional index in AS. These findings have also suggested that JAK-1 and JAK-3 expression may play a role in the inflammatory processes in patients with AS. Keywords: Ankylosing spondylitis, Janus kinase (JAK), Acute-phase reactanthttp://www.sciencedirect.com/science/article/pii/S0929664617305843 |
| spellingShingle | Hsien-Tzung Liao Tzu-Hao Li Chun-Hsiung Chen Hung-An Chen Wei-Sheng Chen Chien-Chih Lai Chung-Tei Chou Chang-Youh Tsai Janus kinase-1 and 3 in ankylosing spondylitis Journal of the Formosan Medical Association |
| title | Janus kinase-1 and 3 in ankylosing spondylitis |
| title_full | Janus kinase-1 and 3 in ankylosing spondylitis |
| title_fullStr | Janus kinase-1 and 3 in ankylosing spondylitis |
| title_full_unstemmed | Janus kinase-1 and 3 in ankylosing spondylitis |
| title_short | Janus kinase-1 and 3 in ankylosing spondylitis |
| title_sort | janus kinase 1 and 3 in ankylosing spondylitis |
| url | http://www.sciencedirect.com/science/article/pii/S0929664617305843 |
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