Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat
Abstract Background Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection. The prevalence and int...
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| Format: | Article |
| Language: | English |
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BMC
2020-10-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-020-04420-8 |
| _version_ | 1819206109559259136 |
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| author | Qinghong Guo Cheng Chen Keke Zhou Yugang Li Laibao Tong Yongcheng Yue Kerou Zhou Jinming Liu Zhiqiang Fu Jiaojiao Lin Jiaxi Zhao Pengxiang Sun Yang Hong |
| author_facet | Qinghong Guo Cheng Chen Keke Zhou Yugang Li Laibao Tong Yongcheng Yue Kerou Zhou Jinming Liu Zhiqiang Fu Jiaojiao Lin Jiaxi Zhao Pengxiang Sun Yang Hong |
| author_sort | Qinghong Guo |
| collection | DOAJ |
| description | Abstract Background Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection. The prevalence and intensity of schistosomiasis infections have significantly decreased in China, and a more sensitive, specific detection method is urgently needed. The aim of this study was to develop a real-time PCR assay for accurate detection of S. japonicum infection in goats. Methods A real-time PCR method for detecting schistosomiasis japonica in goats was developed by amplification of a specific S. japonicum DNA fragment, and validated using a total of 94 negative and 159 positive plasma and serum samples collected in our previous study of S. japonicum infection. Both plasma and serum samples were evaluated by real-time PCR and enzyme-linked immunosorbent assay (ELISA). In addition, 120 goat plasma samples from an S. japonicum-endemic area (Wangjiang) and 33 from a non-endemic region (Weihai) were collected and evaluated using our method. Results The sensitivity and specificity of the real-time PCR for detecting infected samples were 98.74% (157/159, 95% CI: 95.53–99.85%) and 100% (94/94, 95% CI: 96.15–100%), respectively. For the ELISA, sensitivity and specificity were 98.11% (156/159, 95% CI: 94.59–99.61%) and 90.43% (85/94, 95% CI: 82.60–95.53%), respectively. Further, we found positivity rates for S. japonicum infection in Wangjiang and Weihai of 8.33% (10/120, 95% CI: 4.07–14.79%) and 0% (0/33, 95% CI: 0–10.58%), respectively. Conclusions The results of this study indicate that our real-time PCR method exhibits higher sensitivity and specificity than ELISA and is a useful method for detection of S. japonicum infection in goats. |
| first_indexed | 2024-12-23T05:02:22Z |
| format | Article |
| id | doaj.art-5ffa4d4264474dca9896b62e125a21ab |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-23T05:02:22Z |
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| series | Parasites & Vectors |
| spelling | doaj.art-5ffa4d4264474dca9896b62e125a21ab2022-12-21T17:59:11ZengBMCParasites & Vectors1756-33052020-10-011311810.1186/s13071-020-04420-8Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goatQinghong Guo0Cheng Chen1Keke Zhou2Yugang Li3Laibao Tong4Yongcheng Yue5Kerou Zhou6Jinming Liu7Zhiqiang Fu8Jiaojiao Lin9Jiaxi Zhao10Pengxiang Sun11Yang Hong12National Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesHuancui Development Center for Animal HusbandryWangjiang County Center for Animal Disease Control and PreventionNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesWangjiang County Center for Animal Disease Control and PreventionCenter for Disease Control and Prevention of HuancuiNational Reference Laboratory of Animal Schistosomiasis, Key Laboratory of Animal Parasitology of Ministry of Agriculture, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesAbstract Background Schistosomiasis japonica is an infectious disease caused by Schistosoma japonicum that seriously endangers human health. Domestic animals have important roles in disease transmission and goats are considered a primary reservoir host and source of infection. The prevalence and intensity of schistosomiasis infections have significantly decreased in China, and a more sensitive, specific detection method is urgently needed. The aim of this study was to develop a real-time PCR assay for accurate detection of S. japonicum infection in goats. Methods A real-time PCR method for detecting schistosomiasis japonica in goats was developed by amplification of a specific S. japonicum DNA fragment, and validated using a total of 94 negative and 159 positive plasma and serum samples collected in our previous study of S. japonicum infection. Both plasma and serum samples were evaluated by real-time PCR and enzyme-linked immunosorbent assay (ELISA). In addition, 120 goat plasma samples from an S. japonicum-endemic area (Wangjiang) and 33 from a non-endemic region (Weihai) were collected and evaluated using our method. Results The sensitivity and specificity of the real-time PCR for detecting infected samples were 98.74% (157/159, 95% CI: 95.53–99.85%) and 100% (94/94, 95% CI: 96.15–100%), respectively. For the ELISA, sensitivity and specificity were 98.11% (156/159, 95% CI: 94.59–99.61%) and 90.43% (85/94, 95% CI: 82.60–95.53%), respectively. Further, we found positivity rates for S. japonicum infection in Wangjiang and Weihai of 8.33% (10/120, 95% CI: 4.07–14.79%) and 0% (0/33, 95% CI: 0–10.58%), respectively. Conclusions The results of this study indicate that our real-time PCR method exhibits higher sensitivity and specificity than ELISA and is a useful method for detection of S. japonicum infection in goats.http://link.springer.com/article/10.1186/s13071-020-04420-8Schistosoma japonicumDiagnosisReal-time PCRELISAGoats |
| spellingShingle | Qinghong Guo Cheng Chen Keke Zhou Yugang Li Laibao Tong Yongcheng Yue Kerou Zhou Jinming Liu Zhiqiang Fu Jiaojiao Lin Jiaxi Zhao Pengxiang Sun Yang Hong Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat Parasites & Vectors Schistosoma japonicum Diagnosis Real-time PCR ELISA Goats |
| title | Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat |
| title_full | Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat |
| title_fullStr | Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat |
| title_full_unstemmed | Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat |
| title_short | Evaluation of a real-time PCR assay for diagnosis of schistosomiasis japonica in the domestic goat |
| title_sort | evaluation of a real time pcr assay for diagnosis of schistosomiasis japonica in the domestic goat |
| topic | Schistosoma japonicum Diagnosis Real-time PCR ELISA Goats |
| url | http://link.springer.com/article/10.1186/s13071-020-04420-8 |
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