MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production.
MicroRNAs have been shown to contribute to a repertoire of host-pathogen interactions during viral infection. Our previous study demonstrated that microRNA-30e* (miR-30e*) directly targeted the IκBα 3'-UTR and disrupted the NF-κB/IκBα negative feedback loop, leading to hyperactivation of NF-κB....
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-08-01
|
| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | http://europepmc.org/articles/PMC4133224?pdf=render |
| _version_ | 1818197589072281600 |
|---|---|
| author | Xun Zhu Zhenjian He Yiwen Hu Weitao Wen Cuiji Lin Jianchen Yu Jing Pan Ran Li Haijing Deng Shaowei Liao Jie Yuan Jueheng Wu Jun Li Mengfeng Li |
| author_facet | Xun Zhu Zhenjian He Yiwen Hu Weitao Wen Cuiji Lin Jianchen Yu Jing Pan Ran Li Haijing Deng Shaowei Liao Jie Yuan Jueheng Wu Jun Li Mengfeng Li |
| author_sort | Xun Zhu |
| collection | DOAJ |
| description | MicroRNAs have been shown to contribute to a repertoire of host-pathogen interactions during viral infection. Our previous study demonstrated that microRNA-30e* (miR-30e*) directly targeted the IκBα 3'-UTR and disrupted the NF-κB/IκBα negative feedback loop, leading to hyperactivation of NF-κB. This current study investigated the possible role of miR-30e* in the regulation of innate immunity associated with dengue virus (DENV) infection. We found that DENV infection could induce miR-30e* expression in DENV-permissive cells, and such an overexpression of miR-30e* upregulated IFN-β and the downstream IFN-stimulated genes (ISGs) such as OAS1, MxA and IFITM1, and suppressed DENV replication. Furthermore, suppression of IκBα mediates the enhancing effect of miR-30e* on IFN-β-induced antiviral response. Collectively, our findings suggest a modulatory role of miR-30e* in DENV induced IFN-β signaling via the NF-κB-dependent pathway. Further investigation is needed to evaluate whether miR-30e* has an anti-DENV effect in vivo. |
| first_indexed | 2024-12-12T01:52:22Z |
| format | Article |
| id | doaj.art-5ffd630f737242c3990cdac984c693d5 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-12T01:52:22Z |
| publishDate | 2014-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-5ffd630f737242c3990cdac984c693d52022-12-22T00:42:27ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352014-08-0188e308810.1371/journal.pntd.0003088MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production.Xun ZhuZhenjian HeYiwen HuWeitao WenCuiji LinJianchen YuJing PanRan LiHaijing DengShaowei LiaoJie YuanJueheng WuJun LiMengfeng LiMicroRNAs have been shown to contribute to a repertoire of host-pathogen interactions during viral infection. Our previous study demonstrated that microRNA-30e* (miR-30e*) directly targeted the IκBα 3'-UTR and disrupted the NF-κB/IκBα negative feedback loop, leading to hyperactivation of NF-κB. This current study investigated the possible role of miR-30e* in the regulation of innate immunity associated with dengue virus (DENV) infection. We found that DENV infection could induce miR-30e* expression in DENV-permissive cells, and such an overexpression of miR-30e* upregulated IFN-β and the downstream IFN-stimulated genes (ISGs) such as OAS1, MxA and IFITM1, and suppressed DENV replication. Furthermore, suppression of IκBα mediates the enhancing effect of miR-30e* on IFN-β-induced antiviral response. Collectively, our findings suggest a modulatory role of miR-30e* in DENV induced IFN-β signaling via the NF-κB-dependent pathway. Further investigation is needed to evaluate whether miR-30e* has an anti-DENV effect in vivo.http://europepmc.org/articles/PMC4133224?pdf=render |
| spellingShingle | Xun Zhu Zhenjian He Yiwen Hu Weitao Wen Cuiji Lin Jianchen Yu Jing Pan Ran Li Haijing Deng Shaowei Liao Jie Yuan Jueheng Wu Jun Li Mengfeng Li MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production. PLoS Neglected Tropical Diseases |
| title | MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production. |
| title_full | MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production. |
| title_fullStr | MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production. |
| title_full_unstemmed | MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production. |
| title_short | MicroRNA-30e* suppresses dengue virus replication by promoting NF-κB-dependent IFN production. |
| title_sort | microrna 30e suppresses dengue virus replication by promoting nf κb dependent ifn production |
| url | http://europepmc.org/articles/PMC4133224?pdf=render |
| work_keys_str_mv | AT xunzhu microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT zhenjianhe microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT yiwenhu microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT weitaowen microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT cuijilin microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT jianchenyu microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT jingpan microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT ranli microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT haijingdeng microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT shaoweiliao microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT jieyuan microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT juehengwu microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT junli microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction AT mengfengli microrna30esuppressesdenguevirusreplicationbypromotingnfkbdependentifnproduction |