Brain areas lipidomics in female transgenic mouse model of Alzheimer's disease
Abstract Lipids are the major component of the brain with important structural and functional properties. Lipid disruption could play a relevant role in Alzheimer’s disease (AD). Some brain lipidomic studies showed significant differences compared to controls, but few studies have focused on differe...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-01-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-51463-3 |
| _version_ | 1797276543545769984 |
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| author | Laura Ferré-González Ángel Balaguer Marta Roca Artemis Ftara Ana Lloret Consuelo Cháfer-Pericás |
| author_facet | Laura Ferré-González Ángel Balaguer Marta Roca Artemis Ftara Ana Lloret Consuelo Cháfer-Pericás |
| author_sort | Laura Ferré-González |
| collection | DOAJ |
| description | Abstract Lipids are the major component of the brain with important structural and functional properties. Lipid disruption could play a relevant role in Alzheimer’s disease (AD). Some brain lipidomic studies showed significant differences compared to controls, but few studies have focused on different brain areas related to AD. Furthermore, AD is more prevalent in females, but there is a lack of studies focusing on this sex. This work aims to perform a lipidomic study in selected brain areas (cerebellum, amygdala, hippocampus, entire cortex) from wild-type (WT, n = 10) and APPswe/PS1dE9 transgenic (TG, n = 10) female mice of 5 months of age, as a model of early AD, to identify alterations in lipid composition. A lipidomic mass spectrometry-based method was optimized and applied to brain tissue. As result, some lipids showed statistically significant differences between mice groups in cerebellum (n = 68), amygdala (n = 49), hippocampus (n = 48), and the cortex (n = 22). In addition, some lipids (n = 15) from the glycerolipid, phospholipid, and sphingolipid families were statistically significant in several brain areas simultaneously between WT and TG. A selection of lipid variables was made to develop a multivariate approach to assess their discriminant potential, showing high diagnostic indexes, especially in cerebellum and amygdala (sensitivity 70–100%, sensibility 80–100%). |
| first_indexed | 2024-03-07T15:29:40Z |
| format | Article |
| id | doaj.art-5ffdce1e466f45598095dea9258088c2 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:29:40Z |
| publishDate | 2024-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-5ffdce1e466f45598095dea9258088c22024-03-05T16:28:49ZengNature PortfolioScientific Reports2045-23222024-01-0114111110.1038/s41598-024-51463-3Brain areas lipidomics in female transgenic mouse model of Alzheimer's diseaseLaura Ferré-González0Ángel Balaguer1Marta Roca2Artemis Ftara3Ana Lloret4Consuelo Cháfer-Pericás5Alzheimer’s Disease Research Group, Health Research Institute La FeFaculty of Mathematics, University of ValenciaAnalytical Unit, Health Research Institute La FeUniversity of ValenciaDepartment of Physiology, Faculty of Medicine, University of Valencia, Health Research Institute INCLIVAAlzheimer’s Disease Research Group, Health Research Institute La FeAbstract Lipids are the major component of the brain with important structural and functional properties. Lipid disruption could play a relevant role in Alzheimer’s disease (AD). Some brain lipidomic studies showed significant differences compared to controls, but few studies have focused on different brain areas related to AD. Furthermore, AD is more prevalent in females, but there is a lack of studies focusing on this sex. This work aims to perform a lipidomic study in selected brain areas (cerebellum, amygdala, hippocampus, entire cortex) from wild-type (WT, n = 10) and APPswe/PS1dE9 transgenic (TG, n = 10) female mice of 5 months of age, as a model of early AD, to identify alterations in lipid composition. A lipidomic mass spectrometry-based method was optimized and applied to brain tissue. As result, some lipids showed statistically significant differences between mice groups in cerebellum (n = 68), amygdala (n = 49), hippocampus (n = 48), and the cortex (n = 22). In addition, some lipids (n = 15) from the glycerolipid, phospholipid, and sphingolipid families were statistically significant in several brain areas simultaneously between WT and TG. A selection of lipid variables was made to develop a multivariate approach to assess their discriminant potential, showing high diagnostic indexes, especially in cerebellum and amygdala (sensitivity 70–100%, sensibility 80–100%).https://doi.org/10.1038/s41598-024-51463-3 |
| spellingShingle | Laura Ferré-González Ángel Balaguer Marta Roca Artemis Ftara Ana Lloret Consuelo Cháfer-Pericás Brain areas lipidomics in female transgenic mouse model of Alzheimer's disease Scientific Reports |
| title | Brain areas lipidomics in female transgenic mouse model of Alzheimer's disease |
| title_full | Brain areas lipidomics in female transgenic mouse model of Alzheimer's disease |
| title_fullStr | Brain areas lipidomics in female transgenic mouse model of Alzheimer's disease |
| title_full_unstemmed | Brain areas lipidomics in female transgenic mouse model of Alzheimer's disease |
| title_short | Brain areas lipidomics in female transgenic mouse model of Alzheimer's disease |
| title_sort | brain areas lipidomics in female transgenic mouse model of alzheimer s disease |
| url | https://doi.org/10.1038/s41598-024-51463-3 |
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