Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological Activity
Two techniques, namely, optimized ultrasound-assisted extraction (UAE) and enzyme-assisted extraction (EAE), were used to promote the extraction of phenolic compounds from the pseudo-fruits of <i>Rosa canina</i> L. (RC). For UAE, an optimization process based on the design of experiment...
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MDPI AG
2022-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/6/1123 |
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| author | Alexandru Nicolescu Mihai Babotă Leilei Zhang Claudiu I. Bunea Laura Gavrilaș Dan C. Vodnar Andrei Mocan Gianina Crișan Gabriele Rocchetti |
| author_facet | Alexandru Nicolescu Mihai Babotă Leilei Zhang Claudiu I. Bunea Laura Gavrilaș Dan C. Vodnar Andrei Mocan Gianina Crișan Gabriele Rocchetti |
| author_sort | Alexandru Nicolescu |
| collection | DOAJ |
| description | Two techniques, namely, optimized ultrasound-assisted extraction (UAE) and enzyme-assisted extraction (EAE), were used to promote the extraction of phenolic compounds from the pseudo-fruits of <i>Rosa canina</i> L. (RC). For UAE, an optimization process based on the design of experiment (DoE) principles was used for determining the dependence between three variables (i.e., time of extraction, ultrasound amplitude, and the material-to-water ratio) and the total phenolic content of the samples. For EAE, a 2:1:1 pectinase, cellulase, and hemicellulase enzymatic blend was used as pre-treatment for optimized UAE, inducing a higher total phenolic content. The untargeted phenolic profiling approach revealed a great abundance of lower molecular weight phenolics (1.64 mg Eq./g) in UAE-RC extracts, whilst gallic acid (belonging to hydroxybenzoic acid derivatives) was the most abundant individual compound of both extracts. The unsupervised multivariate statistics clearly discriminated the impact of enzymatic pre-treatment on the phenolic profile of RC pseudo-fruits. Finally, Pearson’s correlation coefficients showed that anthocyanins, phenolic acids, and tyrosol derivatives were those compounds mostly correlated to the in vitro antioxidant potential of the extracts, whilst negative and significant (<i>p</i> < 0.05) correlation coefficients were recorded when considering the enzymatic inhibition activities. The highest enzyme-inhibitory activity has been identified against α-glucosidase, which indicates an antidiabetic effect. |
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| language | English |
| last_indexed | 2024-03-10T00:34:24Z |
| publishDate | 2022-06-01 |
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| series | Antioxidants |
| spelling | doaj.art-5fff8d3b415741dbb6cd15df8dafc29f2023-11-23T15:19:59ZengMDPI AGAntioxidants2076-39212022-06-01116112310.3390/antiox11061123Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological ActivityAlexandru Nicolescu0Mihai Babotă1Leilei Zhang2Claudiu I. Bunea3Laura Gavrilaș4Dan C. Vodnar5Andrei Mocan6Gianina Crișan7Gabriele Rocchetti8Department of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, RomaniaDepartment for Sustainable Food Process, Università Cattolica del Sacro Cuore, 29122 Piacenza, ItalyFaculty of Horticulture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăştur 3-5, 400372 Cluj-Napoca, RomaniaDepartment of Bromatology, Hygiene, Nutrition, “Iuliu Haţieganu” University of Medicine and Pharmacy, 6 Pasteur Street, 400349 Cluj-Napoca, RomaniaInstitute of Life Sciences, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 400372 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, RomaniaDepartment of Pharmaceutical Botany, “Iuliu Hațieganu” University of Medicine and Pharmacy, Gheorghe Marinescu Street 23, 400337 Cluj-Napoca, RomaniaDepartment of Animal Science, Food and Nutrition, Università Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, ItalyTwo techniques, namely, optimized ultrasound-assisted extraction (UAE) and enzyme-assisted extraction (EAE), were used to promote the extraction of phenolic compounds from the pseudo-fruits of <i>Rosa canina</i> L. (RC). For UAE, an optimization process based on the design of experiment (DoE) principles was used for determining the dependence between three variables (i.e., time of extraction, ultrasound amplitude, and the material-to-water ratio) and the total phenolic content of the samples. For EAE, a 2:1:1 pectinase, cellulase, and hemicellulase enzymatic blend was used as pre-treatment for optimized UAE, inducing a higher total phenolic content. The untargeted phenolic profiling approach revealed a great abundance of lower molecular weight phenolics (1.64 mg Eq./g) in UAE-RC extracts, whilst gallic acid (belonging to hydroxybenzoic acid derivatives) was the most abundant individual compound of both extracts. The unsupervised multivariate statistics clearly discriminated the impact of enzymatic pre-treatment on the phenolic profile of RC pseudo-fruits. Finally, Pearson’s correlation coefficients showed that anthocyanins, phenolic acids, and tyrosol derivatives were those compounds mostly correlated to the in vitro antioxidant potential of the extracts, whilst negative and significant (<i>p</i> < 0.05) correlation coefficients were recorded when considering the enzymatic inhibition activities. The highest enzyme-inhibitory activity has been identified against α-glucosidase, which indicates an antidiabetic effect.https://www.mdpi.com/2076-3921/11/6/1123<i>Rosa canina</i>UAEEAEantioxidantsDoEphenolic profiling |
| spellingShingle | Alexandru Nicolescu Mihai Babotă Leilei Zhang Claudiu I. Bunea Laura Gavrilaș Dan C. Vodnar Andrei Mocan Gianina Crișan Gabriele Rocchetti Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological Activity Antioxidants <i>Rosa canina</i> UAE EAE antioxidants DoE phenolic profiling |
| title | Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological Activity |
| title_full | Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological Activity |
| title_fullStr | Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological Activity |
| title_full_unstemmed | Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological Activity |
| title_short | Optimized Ultrasound-Assisted Enzymatic Extraction of Phenolic Compounds from <i>Rosa canina</i> L. Pseudo-Fruits (Rosehip) and Their Biological Activity |
| title_sort | optimized ultrasound assisted enzymatic extraction of phenolic compounds from i rosa canina i l pseudo fruits rosehip and their biological activity |
| topic | <i>Rosa canina</i> UAE EAE antioxidants DoE phenolic profiling |
| url | https://www.mdpi.com/2076-3921/11/6/1123 |
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