Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)
We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier...
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612100310X |
| _version_ | 1818903895036919808 |
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| author | Sandra Ropret Jakob Jeriha Tjaša Sorčan Marija Rogar Špela Zemljič Jokhadar John A. McGrath Dusko Ilic Mirjana Liovic |
| author_facet | Sandra Ropret Jakob Jeriha Tjaša Sorčan Marija Rogar Špela Zemljič Jokhadar John A. McGrath Dusko Ilic Mirjana Liovic |
| author_sort | Sandra Ropret |
| collection | DOAJ |
| description | We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts. |
| first_indexed | 2024-12-19T20:58:48Z |
| format | Article |
| id | doaj.art-60019e7485d147578d84f6b048019b8a |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-19T20:58:48Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-60019e7485d147578d84f6b048019b8a2022-12-21T20:05:53ZengElsevierStem Cell Research1873-50612021-08-0155102463Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB)Sandra Ropret0Jakob Jeriha1Tjaša Sorčan2Marija Rogar3Špela Zemljič Jokhadar4John A. McGrath5Dusko Ilic6Mirjana Liovic7Medical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, SloveniaStem Cell Lab, Department of Women and Children’s Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy’s Hospital, London, UKMedical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, SloveniaMedical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia; AdriaBio doo, Postojna, SloveniaInstitute of Biophysics, Faculty of Medicine, University of Ljubljana, SloveniaGenetic Skin Disease Group, St John’s Institute of Dermatology, Guy’s Hospital, London, UKStem Cell Lab, Department of Women and Children’s Health, School of Life Course Sciences, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guy’s Hospital, London, UKMedical Center for Molecular Biology, Faculty of Medicine, University of Ljubljana, Slovenia; Corresponding author.We have generated MLi004-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a female patient with recessive dystrophic epidermolysis bullosa (RDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi004-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-004A line has been also tested for ability to differentiate into fibroblasts.http://www.sciencedirect.com/science/article/pii/S187350612100310XCollagen VIIRecessive dystrophic EBiPSC linePluripotencyFibroblastKeratinocyte |
| spellingShingle | Sandra Ropret Jakob Jeriha Tjaša Sorčan Marija Rogar Špela Zemljič Jokhadar John A. McGrath Dusko Ilic Mirjana Liovic Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB) Stem Cell Research Collagen VII Recessive dystrophic EB iPSC line Pluripotency Fibroblast Keratinocyte |
| title | Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB) |
| title_full | Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB) |
| title_fullStr | Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB) |
| title_full_unstemmed | Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB) |
| title_short | Induced pluripotent stem cell (iPSC) line MLi-004A derived from a patient with recessive dystrophic epidermolysis bullosa (RDEB) |
| title_sort | induced pluripotent stem cell ipsc line mli 004a derived from a patient with recessive dystrophic epidermolysis bullosa rdeb |
| topic | Collagen VII Recessive dystrophic EB iPSC line Pluripotency Fibroblast Keratinocyte |
| url | http://www.sciencedirect.com/science/article/pii/S187350612100310X |
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