Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant Pathogens
Continuous cropping is the main form of cultivation in Chinese agriculture. The bacterial community plays an important role in maintaining the healthy growth of plants. However, there are few reports on the composition and dynamics of the bacterial community structure under continuous cropping of &l...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-01-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/14/2/260 |
| _version_ | 1827344393067036672 |
|---|---|
| author | Qingyun Ma Lin Li Xiaoyan Han Xu Jiang Yiqing Zhou Tan Wang Yan Zhuang Zhiyong Ruan |
| author_facet | Qingyun Ma Lin Li Xiaoyan Han Xu Jiang Yiqing Zhou Tan Wang Yan Zhuang Zhiyong Ruan |
| author_sort | Qingyun Ma |
| collection | DOAJ |
| description | Continuous cropping is the main form of cultivation in Chinese agriculture. The bacterial community plays an important role in maintaining the healthy growth of plants. However, there are few reports on the composition and dynamics of the bacterial community structure under continuous cropping of <i>Lonicera japonica</i> Thunb. High-throughput sequencing was used to monitor the variation in the soil bacterial community structure of different monocropping years of <i>Lonicera japonica</i> Thunb., as well as the correlation between soil characteristics and bacterial community. Meanwhile, antagonistic bacteria for <i>Fusarium oxysporum</i> pathogens were isolated and functionally verified by culture-dependent techniques and pot experiments. Bacterial community diversity and structure changed significantly with the increase in the years of <i>Lonicera japonica</i> Thunb. succession. However, some beneficial bacteria, such as <i>Bacillus</i> and <i>Nitrosospira</i>, were gradually depleted. The complexity of the bacteria co-occurrence networks decreased with increasing years of cropping. FAPROTAX-based functional prediction showed that the abundance of genes related to carbon, nitrogen, sulfur metabolism and chitinlysis were reduced with the extended crop succession. Furthermore, the three <i>Bacillus</i> strains that were strongly antagonistic toward <i>Fusarium oxysporum</i> and the pot experiment demonstrated they significantly promoted <i>Lonicera japonica</i> Thunb. growth. Our research provides theoretical support for the development of microbial fertilizers that are beneficial to plants. |
| first_indexed | 2024-03-07T22:46:34Z |
| format | Article |
| id | doaj.art-600532a1a28b4099a5e219aea872d084 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-07T22:46:34Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-600532a1a28b4099a5e219aea872d0842024-02-23T15:04:00ZengMDPI AGAgronomy2073-43952024-01-0114226010.3390/agronomy14020260Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant PathogensQingyun Ma0Lin Li1Xiaoyan Han2Xu Jiang3Yiqing Zhou4Tan Wang5Yan Zhuang6Zhiyong Ruan7National Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaNational Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, ChinaInstitute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaContinuous cropping is the main form of cultivation in Chinese agriculture. The bacterial community plays an important role in maintaining the healthy growth of plants. However, there are few reports on the composition and dynamics of the bacterial community structure under continuous cropping of <i>Lonicera japonica</i> Thunb. High-throughput sequencing was used to monitor the variation in the soil bacterial community structure of different monocropping years of <i>Lonicera japonica</i> Thunb., as well as the correlation between soil characteristics and bacterial community. Meanwhile, antagonistic bacteria for <i>Fusarium oxysporum</i> pathogens were isolated and functionally verified by culture-dependent techniques and pot experiments. Bacterial community diversity and structure changed significantly with the increase in the years of <i>Lonicera japonica</i> Thunb. succession. However, some beneficial bacteria, such as <i>Bacillus</i> and <i>Nitrosospira</i>, were gradually depleted. The complexity of the bacteria co-occurrence networks decreased with increasing years of cropping. FAPROTAX-based functional prediction showed that the abundance of genes related to carbon, nitrogen, sulfur metabolism and chitinlysis were reduced with the extended crop succession. Furthermore, the three <i>Bacillus</i> strains that were strongly antagonistic toward <i>Fusarium oxysporum</i> and the pot experiment demonstrated they significantly promoted <i>Lonicera japonica</i> Thunb. growth. Our research provides theoretical support for the development of microbial fertilizers that are beneficial to plants.https://www.mdpi.com/2073-4395/14/2/260<i>Lonicera japonica</i> Thunb.microbial community structurecontinuous croppingbeneficial microbes<i>Bacillus</i> |
| spellingShingle | Qingyun Ma Lin Li Xiaoyan Han Xu Jiang Yiqing Zhou Tan Wang Yan Zhuang Zhiyong Ruan Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant Pathogens Agronomy <i>Lonicera japonica</i> Thunb. microbial community structure continuous cropping beneficial microbes <i>Bacillus</i> |
| title | Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant Pathogens |
| title_full | Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant Pathogens |
| title_fullStr | Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant Pathogens |
| title_full_unstemmed | Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant Pathogens |
| title_short | Structural and Functional Analysis of the Bacterial Community in the Soil of Continuously Cultivated <i>Lonicera japonica</i> Thunb. and Screening Antagonistic Bacteria for Plant Pathogens |
| title_sort | structural and functional analysis of the bacterial community in the soil of continuously cultivated i lonicera japonica i thunb and screening antagonistic bacteria for plant pathogens |
| topic | <i>Lonicera japonica</i> Thunb. microbial community structure continuous cropping beneficial microbes <i>Bacillus</i> |
| url | https://www.mdpi.com/2073-4395/14/2/260 |
| work_keys_str_mv | AT qingyunma structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens AT linli structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens AT xiaoyanhan structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens AT xujiang structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens AT yiqingzhou structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens AT tanwang structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens AT yanzhuang structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens AT zhiyongruan structuralandfunctionalanalysisofthebacterialcommunityinthesoilofcontinuouslycultivatedilonicerajaponicaithunbandscreeningantagonisticbacteriaforplantpathogens |