Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach
The metabolite profile of fresh Goji berries from two cultivars, namely Big Lifeberry (BL) and Sweet Lifeberry (SL), grown in the Lazio region (Central Italy) and harvested at two different periods, August and October, corresponding at the beginning and the end of the maturation, was characterized b...
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MDPI AG
2021-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/26/17/5412 |
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| author | Mattia Spano Alessandro Maccelli Giacomo Di Matteo Cinzia Ingallina Mariangela Biava Maria Elisa Crestoni Jean-Xavier Bardaud Anna Maria Giusti Alessia Mariano Anna Scotto D’Abusco Anatoly P. Sobolev Alba Lasalvia Simonetta Fornarini Luisa Mannina |
| author_facet | Mattia Spano Alessandro Maccelli Giacomo Di Matteo Cinzia Ingallina Mariangela Biava Maria Elisa Crestoni Jean-Xavier Bardaud Anna Maria Giusti Alessia Mariano Anna Scotto D’Abusco Anatoly P. Sobolev Alba Lasalvia Simonetta Fornarini Luisa Mannina |
| author_sort | Mattia Spano |
| collection | DOAJ |
| description | The metabolite profile of fresh Goji berries from two cultivars, namely Big Lifeberry (BL) and Sweet Lifeberry (SL), grown in the Lazio region (Central Italy) and harvested at two different periods, August and October, corresponding at the beginning and the end of the maturation, was characterized by means of nuclear magnetic resonance (NMR) and electrospray ionization Fourier transform ion cyclotron resonance (ESI FT-ICR MS) methodologies. Several classes of compounds such as sugars, amino acids, organic acids, fatty acids, polyphenols, and terpenes were identified and quantified in hydroalcoholic and organic Bligh-Dyer extracts. Sweet Lifeberry extracts were characterized by a higher content of sucrose with respect to the Big Lifeberry ones and high levels of amino acids (glycine, betaine, proline) were observed in SL berries harvested in October. Spectrophotometric analysis of chlorophylls and total carotenoids was also carried out, showing a decrease of carotenoids during the time. These results can be useful not only to valorize local products but also to suggest the best harvesting period to obtain a product with a chemical composition suitable for specific industrial use. Finally, preliminary studies regarding both the chemical characterization of Goji leaves generally considered a waste product, and the biological activity of Big Lifeberry berries extracts was also investigated. Goji leaves showed a chemical profile rich in healthy compounds (polyphenols, flavonoids, etc.) confirming their promising use in the supplements/nutraceutical/cosmetic field. MG63 cells treated with Big Lifeberry berries extracts showed a decrease of iNOS, COX-2, IL-6, and IL-8 expression indicating their significant biological activity. |
| first_indexed | 2024-03-10T08:05:52Z |
| format | Article |
| id | doaj.art-6006779fce3a49aeb64db07ef0ad7d63 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T08:05:52Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6006779fce3a49aeb64db07ef0ad7d632023-11-22T11:03:50ZengMDPI AGMolecules1420-30492021-09-012617541210.3390/molecules26175412Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological ApproachMattia Spano0Alessandro Maccelli1Giacomo Di Matteo2Cinzia Ingallina3Mariangela Biava4Maria Elisa Crestoni5Jean-Xavier Bardaud6Anna Maria Giusti7Alessia Mariano8Anna Scotto D’Abusco9Anatoly P. Sobolev10Alba Lasalvia11Simonetta Fornarini12Luisa Mannina13Department of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyInstitut de Chimie Physique, CLIO, Université Paris Saclay, Bât 200, BP34, CEDEX, 91898 Orsay, FranceDepartment of Experimental Medicine, Sapienza University of Rome, P.le Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, Sapienza University of Roma, P.le Aldo Moro 5, 00185 Rome, ItalyDepartment of Biochemical Sciences, Sapienza University of Roma, P.le Aldo Moro 5, 00185 Rome, ItalyInstitute for Biological Systems, Magnetic Resonance Laboratory “Segre-Capitani”, CNR, Via Salaria Km 29.300, 00015 Monterotondo, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyDepartment of Chemistry and Technology of Drugs, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, ItalyThe metabolite profile of fresh Goji berries from two cultivars, namely Big Lifeberry (BL) and Sweet Lifeberry (SL), grown in the Lazio region (Central Italy) and harvested at two different periods, August and October, corresponding at the beginning and the end of the maturation, was characterized by means of nuclear magnetic resonance (NMR) and electrospray ionization Fourier transform ion cyclotron resonance (ESI FT-ICR MS) methodologies. Several classes of compounds such as sugars, amino acids, organic acids, fatty acids, polyphenols, and terpenes were identified and quantified in hydroalcoholic and organic Bligh-Dyer extracts. Sweet Lifeberry extracts were characterized by a higher content of sucrose with respect to the Big Lifeberry ones and high levels of amino acids (glycine, betaine, proline) were observed in SL berries harvested in October. Spectrophotometric analysis of chlorophylls and total carotenoids was also carried out, showing a decrease of carotenoids during the time. These results can be useful not only to valorize local products but also to suggest the best harvesting period to obtain a product with a chemical composition suitable for specific industrial use. Finally, preliminary studies regarding both the chemical characterization of Goji leaves generally considered a waste product, and the biological activity of Big Lifeberry berries extracts was also investigated. Goji leaves showed a chemical profile rich in healthy compounds (polyphenols, flavonoids, etc.) confirming their promising use in the supplements/nutraceutical/cosmetic field. MG63 cells treated with Big Lifeberry berries extracts showed a decrease of iNOS, COX-2, IL-6, and IL-8 expression indicating their significant biological activity.https://www.mdpi.com/1420-3049/26/17/5412Goji berriesmetabolite profileNMRFT-ICR MSGoji leavesbiological assays |
| spellingShingle | Mattia Spano Alessandro Maccelli Giacomo Di Matteo Cinzia Ingallina Mariangela Biava Maria Elisa Crestoni Jean-Xavier Bardaud Anna Maria Giusti Alessia Mariano Anna Scotto D’Abusco Anatoly P. Sobolev Alba Lasalvia Simonetta Fornarini Luisa Mannina Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach Molecules Goji berries metabolite profile NMR FT-ICR MS Goji leaves biological assays |
| title | Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach |
| title_full | Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach |
| title_fullStr | Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach |
| title_full_unstemmed | Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach |
| title_short | Metabolomic Profiling of Fresh Goji (<i>Lycium barbarum</i> L.) Berries from Two Cultivars Grown in Central Italy: A Multi-Methodological Approach |
| title_sort | metabolomic profiling of fresh goji i lycium barbarum i l berries from two cultivars grown in central italy a multi methodological approach |
| topic | Goji berries metabolite profile NMR FT-ICR MS Goji leaves biological assays |
| url | https://www.mdpi.com/1420-3049/26/17/5412 |
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