IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)

IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)

<!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --> <p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"><span style="color: #000000...

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Main Authors: Alexandre Bosco de Oliveira, Fernando Felipe Ferreyra Hernandez, Josefa Diva Nogueira Diniz, Jacqueline Leite Almeida
Format: Article
Language:English
Published: Universidade Federal de Goiás 2010-05-01
Series:Pesquisa Agropecuária Tropical
Subjects:
Desinfecção
regulador de crescimento
calos
enraizamento
Online Access:http://www.revistas.ufg.br/index.php/pat/article/view/3956
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author Alexandre Bosco de Oliveira
Fernando Felipe Ferreyra Hernandez
Josefa Diva Nogueira Diniz
Jacqueline Leite Almeida
author_facet Alexandre Bosco de Oliveira
Fernando Felipe Ferreyra Hernandez
Josefa Diva Nogueira Diniz
Jacqueline Leite Almeida
author_sort Alexandre Bosco de Oliveira
collection DOAJ
description &lt;!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;This research aimed to develop a protocol for the micropropagation of &lt;em&gt;Mussaenda erytrophylla&lt;/em&gt; cv. Rosea. Calcium hypochlorite or sodium hypochlorite (2.5%) was used for the sterilization of bracts segments, with or without previous immersion in alcohol 70%. For callus induction, bracts segments were inoculated on MS medium with different concentrations of BAP and NAA. For buds induction, callus were inoculated on MS medium with different concentrations of cytokinins BAP, 2iP, and KIN (0.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.5 mg L&lt;sup&gt;-1&lt;/sup&gt;, 1.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, and 2.0 mg L&lt;sup&gt;-1&lt;/sup&gt;). For multiplication, nodal segments from &lt;em&gt;in vitro&lt;/em&gt; plants were inoculated in MS medium with BAP, KIN, and 2iP, in the concentrations of 0.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.5 mg L&lt;sup&gt;-1&lt;/sup&gt;, 1.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, and 2.0 mg L&lt;sup&gt;-1&lt;/sup&gt;. For rooting, nodal segments from &lt;em&gt;in vitro&lt;/em&gt; plants were inoculated using two MS medium dilutions: full strength and half strength of MS basal salt mixture (macronutrients salts) and different concentrations of IBA (0.00 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.25 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.50 mg L&lt;sup&gt;-1&lt;/sup&gt;, and 1.00 mg L&lt;sup&gt;-1&lt;/sup&gt;). The best sterilization treatment was achieved by using calcium hypochlorite combined with immersion in alcohol. For callus induction, the MS medium with 0.4 mg L&lt;sup&gt;-1&lt;/sup&gt; of NAA plus 4.0 mg L&lt;sup&gt;-1 &lt;/sup&gt;of BAP was more efficient. Bud multiplication was improved in MS medium with 0.5 mg L&lt;sup&gt;-1&lt;/sup&gt; of 2iP or 1.0 mg L&lt;sup&gt;-1&lt;/sup&gt; of KIN. The highest percentage of explants with roots without emission of callus was observed in the medium with 50% and 100% of MS salts without IBA.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="en-US" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;KEY-WORDS: Disinfection; growth regulator; callus; rooting. &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;<br>&lt;!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;O trabalho foi realizado visando a desenvolver um protocolo para micropropagação de &lt;em&gt;Mussaenda erytrophylla&lt;/em&gt; cv. Rosea. Na assepsia de segmentos de brácteas, utilizou-se hipoclorito de cálcio ou hipoclorito de sódio (2,5%), com ou sem imersão prévia em álcool 70%. Para indução de calos, os segmentos de brácteas foram inoculados em meio MS com diferentes concentrações de BAP e ANA. Para indução de gemas, os calos foram inoculados em meio MS com diferentes concentrações das citocininas BAP, 2iP e CIN (0,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,5 mg L&lt;sup&gt;-1&lt;/sup&gt;; 1,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; e 2,0 mg L&lt;sup&gt;-1&lt;/sup&gt;). Na multiplicação, segmentos nodais de plantas &lt;em&gt;in vitro&lt;/em&gt; foram inoculados em meio MS com BAP, CIN e 2iP, nas concentrações de 0,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,5 mg L&lt;sup&gt;-1&lt;/sup&gt;; 1,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; e 2,0 mg L&lt;sup&gt;-1&lt;/sup&gt;. Para o enraizamento, segmentos nodais de plantas &lt;em&gt;in vitro &lt;/em&gt;foram inoculados no meio MS e MS/2 da formulação dos sais macronutrientes e diferentes concentrações de AIB (0,00 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,25 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,50 mg L&lt;sup&gt;-1&lt;/sup&gt;; e 1,00 mg L&lt;sup&gt;-1&lt;/sup&gt;). O melhor tratamento para a desinfestação foi aquele em que utilizou-se hipoclorito de cálcio associado à imersão em álcool. Para a indução de calos, o meio MS com 0,4 mg L&lt;sup&gt;-1&lt;/sup&gt; de ANA + 4,0 mg L&lt;sup&gt;-1&lt;/sup&gt; de BAP mostrou-se o mais efetivo. A multiplicação das gemas foi favorecida em meio MS com 0,5 mg L&lt;sup&gt;-1&lt;/sup&gt; de 2iP ou com 1,0 mg L&lt;sup&gt;-1&lt;/sup&gt; de CIN. A maior percentagem de explantes com raízes sem a formação de calos foi observada nos meios com 50% e 100% dos sais MS sem AIB.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;PALAVRAS-CHAVE: Desinfecção; regulador de crescimento; calos; enraizamento.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p class="western" style="margin-bottom: 0cm;" align="JUSTIFY"&gt; &lt;/p&gt;
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publishDate 2010-05-01
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series Pesquisa Agropecuária Tropical
spelling doaj.art-60070c3e46094f46b8e340f8135e6a372022-12-22T01:17:52ZengUniversidade Federal de GoiásPesquisa Agropecuária Tropical1983-40632010-05-0140210.5216/pat.v40i2.3956IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)Alexandre Bosco de OliveiraFernando Felipe Ferreyra HernandezJosefa Diva Nogueira DinizJacqueline Leite Almeida&lt;!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;This research aimed to develop a protocol for the micropropagation of &lt;em&gt;Mussaenda erytrophylla&lt;/em&gt; cv. Rosea. Calcium hypochlorite or sodium hypochlorite (2.5%) was used for the sterilization of bracts segments, with or without previous immersion in alcohol 70%. For callus induction, bracts segments were inoculated on MS medium with different concentrations of BAP and NAA. For buds induction, callus were inoculated on MS medium with different concentrations of cytokinins BAP, 2iP, and KIN (0.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.5 mg L&lt;sup&gt;-1&lt;/sup&gt;, 1.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, and 2.0 mg L&lt;sup&gt;-1&lt;/sup&gt;). For multiplication, nodal segments from &lt;em&gt;in vitro&lt;/em&gt; plants were inoculated in MS medium with BAP, KIN, and 2iP, in the concentrations of 0.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.5 mg L&lt;sup&gt;-1&lt;/sup&gt;, 1.0 mg L&lt;sup&gt;-1&lt;/sup&gt;, and 2.0 mg L&lt;sup&gt;-1&lt;/sup&gt;. For rooting, nodal segments from &lt;em&gt;in vitro&lt;/em&gt; plants were inoculated using two MS medium dilutions: full strength and half strength of MS basal salt mixture (macronutrients salts) and different concentrations of IBA (0.00 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.25 mg L&lt;sup&gt;-1&lt;/sup&gt;, 0.50 mg L&lt;sup&gt;-1&lt;/sup&gt;, and 1.00 mg L&lt;sup&gt;-1&lt;/sup&gt;). The best sterilization treatment was achieved by using calcium hypochlorite combined with immersion in alcohol. For callus induction, the MS medium with 0.4 mg L&lt;sup&gt;-1&lt;/sup&gt; of NAA plus 4.0 mg L&lt;sup&gt;-1 &lt;/sup&gt;of BAP was more efficient. Bud multiplication was improved in MS medium with 0.5 mg L&lt;sup&gt;-1&lt;/sup&gt; of 2iP or 1.0 mg L&lt;sup&gt;-1&lt;/sup&gt; of KIN. The highest percentage of explants with roots without emission of callus was observed in the medium with 50% and 100% of MS salts without IBA.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="en-US" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;KEY-WORDS: Disinfection; growth regulator; callus; rooting. &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt;<br>&lt;!-- @page { margin: 2cm } P { margin-bottom: 0.21cm } --&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;O trabalho foi realizado visando a desenvolver um protocolo para micropropagação de &lt;em&gt;Mussaenda erytrophylla&lt;/em&gt; cv. Rosea. Na assepsia de segmentos de brácteas, utilizou-se hipoclorito de cálcio ou hipoclorito de sódio (2,5%), com ou sem imersão prévia em álcool 70%. Para indução de calos, os segmentos de brácteas foram inoculados em meio MS com diferentes concentrações de BAP e ANA. Para indução de gemas, os calos foram inoculados em meio MS com diferentes concentrações das citocininas BAP, 2iP e CIN (0,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,5 mg L&lt;sup&gt;-1&lt;/sup&gt;; 1,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; e 2,0 mg L&lt;sup&gt;-1&lt;/sup&gt;). Na multiplicação, segmentos nodais de plantas &lt;em&gt;in vitro&lt;/em&gt; foram inoculados em meio MS com BAP, CIN e 2iP, nas concentrações de 0,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,5 mg L&lt;sup&gt;-1&lt;/sup&gt;; 1,0 mg L&lt;sup&gt;-1&lt;/sup&gt;; e 2,0 mg L&lt;sup&gt;-1&lt;/sup&gt;. Para o enraizamento, segmentos nodais de plantas &lt;em&gt;in vitro &lt;/em&gt;foram inoculados no meio MS e MS/2 da formulação dos sais macronutrientes e diferentes concentrações de AIB (0,00 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,25 mg L&lt;sup&gt;-1&lt;/sup&gt;; 0,50 mg L&lt;sup&gt;-1&lt;/sup&gt;; e 1,00 mg L&lt;sup&gt;-1&lt;/sup&gt;). O melhor tratamento para a desinfestação foi aquele em que utilizou-se hipoclorito de cálcio associado à imersão em álcool. Para a indução de calos, o meio MS com 0,4 mg L&lt;sup&gt;-1&lt;/sup&gt; de ANA + 4,0 mg L&lt;sup&gt;-1&lt;/sup&gt; de BAP mostrou-se o mais efetivo. A multiplicação das gemas foi favorecida em meio MS com 0,5 mg L&lt;sup&gt;-1&lt;/sup&gt; de 2iP ou com 1,0 mg L&lt;sup&gt;-1&lt;/sup&gt; de CIN. A maior percentagem de explantes com raízes sem a formação de calos foi observada nos meios com 50% e 100% dos sais MS sem AIB.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p style="margin-bottom: 0cm; font-style: normal; font-weight: normal; line-height: 120%; text-decoration: none;" lang="pt-BR" align="JUSTIFY"&gt;&lt;span style="color: #000000;"&gt;&lt;span style="font-family: Times New Roman,serif;"&gt;&lt;span style="font-size: small;"&gt;PALAVRAS-CHAVE: Desinfecção; regulador de crescimento; calos; enraizamento.&lt;/span&gt;&lt;/span&gt;&lt;/span&gt;&lt;/p&gt; &lt;p class="western" style="margin-bottom: 0cm;" align="JUSTIFY"&gt; &lt;/p&gt;http://www.revistas.ufg.br/index.php/pat/article/view/3956Desinfecçãoregulador de crescimentocalosenraizamento
spellingShingle Alexandre Bosco de Oliveira
Fernando Felipe Ferreyra Hernandez
Josefa Diva Nogueira Diniz
Jacqueline Leite Almeida
IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)
Pesquisa Agropecuária Tropical
Desinfecção
regulador de crescimento
calos
enraizamento
title IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)
title_full IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)
title_fullStr IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)
title_full_unstemmed IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)
title_short IN VITRO PROPAGATION OF MUSSAENDA (Mussaenda erythrophylla cv. Rosea) PROPAGAÇÃO IN VITRO DE MUSSAENDA (Mussaenda erythrophylla cv. Rosea)
title_sort in vitro propagation of mussaenda mussaenda erythrophylla cv rosea propagacao in vitro de mussaenda mussaenda erythrophylla cv rosea
topic Desinfecção
regulador de crescimento
calos
enraizamento
url http://www.revistas.ufg.br/index.php/pat/article/view/3956
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AT jacquelineleitealmeida invitropropagationofmussaendamussaendaerythrophyllacvroseapropagacaoinvitrodemussaendamussaendaerythrophyllacvrosea

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