Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots
The main role of RALF small signaling peptides was reported to be the alkalization control of the apoplast for improvement of nutrient absorption; however, the exact function of individual RALF peptides such as RALF34 remains unknown. The Arabidopsis RALF34 (<i>At</i>RALF34) peptide was...
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2023-04-01
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| Series: | International Journal of Molecular Sciences |
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| author | Julia Shumilina Alexey S. Kiryushkin Nadezhda Frolova Valeria Mashkina Elena L. Ilina Vera A. Puchkova Katerina Danko Svetlana Silinskaya Evgeny B. Serebryakov Alena Soboleva Tatiana Bilova Anastasia Orlova Elizaveta D. Guseva Egor Repkin Katharina Pawlowski Andrej Frolov Kirill N. Demchenko |
| author_facet | Julia Shumilina Alexey S. Kiryushkin Nadezhda Frolova Valeria Mashkina Elena L. Ilina Vera A. Puchkova Katerina Danko Svetlana Silinskaya Evgeny B. Serebryakov Alena Soboleva Tatiana Bilova Anastasia Orlova Elizaveta D. Guseva Egor Repkin Katharina Pawlowski Andrej Frolov Kirill N. Demchenko |
| author_sort | Julia Shumilina |
| collection | DOAJ |
| description | The main role of RALF small signaling peptides was reported to be the alkalization control of the apoplast for improvement of nutrient absorption; however, the exact function of individual RALF peptides such as RALF34 remains unknown. The Arabidopsis RALF34 (<i>At</i>RALF34) peptide was proposed to be part of the gene regulatory network of lateral root initiation. Cucumber is an excellent model for studying a special form of lateral root initiation taking place in the meristem of the parental root. We attempted to elucidate the role of the regulatory pathway in which RALF34 is a participant using cucumber transgenic hairy roots overexpressing <i>CsRALF34</i> for comprehensive, integrated metabolomics and proteomics studies, focusing on the analysis of stress response markers. <i>Cs</i>RALF34 overexpression resulted in the inhibition of root growth and regulation of cell proliferation, specifically in blocking the G2/M transition in cucumber roots. Based on these results, we propose that <i>CsRALF34</i> is not part of the gene regulatory networks involved in the early steps of lateral root initiation. Instead, we suggest that <i>Cs</i>RALF34 modulates ROS homeostasis and triggers the controlled production of hydroxyl radicals in root cells, possibly associated with intracellular signal transduction. Altogether, our results support the role of RALF peptides as ROS regulators. |
| first_indexed | 2024-03-11T04:55:06Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:55:06Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-600960858b8848b0b73fd67390cd087f2023-11-17T19:43:47ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-04-01248765410.3390/ijms24087654Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber RootsJulia Shumilina0Alexey S. Kiryushkin1Nadezhda Frolova2Valeria Mashkina3Elena L. Ilina4Vera A. Puchkova5Katerina Danko6Svetlana Silinskaya7Evgeny B. Serebryakov8Alena Soboleva9Tatiana Bilova10Anastasia Orlova11Elizaveta D. Guseva12Egor Repkin13Katharina Pawlowski14Andrej Frolov15Kirill N. Demchenko16Saint Petersburg State University, 199034 Saint Petersburg, RussiaLaboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint Petersburg, RussiaSaint Petersburg State University, 199034 Saint Petersburg, RussiaSaint Petersburg State University, 199034 Saint Petersburg, RussiaLaboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint Petersburg, RussiaLaboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint Petersburg, RussiaSaint Petersburg State University, 199034 Saint Petersburg, RussiaSaint Petersburg State University, 199034 Saint Petersburg, RussiaSaint Petersburg State University, 199034 Saint Petersburg, RussiaLaboratory of Analytical Biochemistry and Biotechnology, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaSaint Petersburg State University, 199034 Saint Petersburg, RussiaLaboratory of Analytical Biochemistry and Biotechnology, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaLaboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint Petersburg, RussiaSaint Petersburg State University, 199034 Saint Petersburg, RussiaDepartment of Ecology, Environment and Plant Sciences, Stockholm University, 10691 Stockholm, SwedenLaboratory of Analytical Biochemistry and Biotechnology, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, 127276 Moscow, RussiaLaboratory of Cellular and Molecular Mechanisms of Plant Development, Komarov Botanical Institute, Russian Academy of Sciences, 197022 Saint Petersburg, RussiaThe main role of RALF small signaling peptides was reported to be the alkalization control of the apoplast for improvement of nutrient absorption; however, the exact function of individual RALF peptides such as RALF34 remains unknown. The Arabidopsis RALF34 (<i>At</i>RALF34) peptide was proposed to be part of the gene regulatory network of lateral root initiation. Cucumber is an excellent model for studying a special form of lateral root initiation taking place in the meristem of the parental root. We attempted to elucidate the role of the regulatory pathway in which RALF34 is a participant using cucumber transgenic hairy roots overexpressing <i>CsRALF34</i> for comprehensive, integrated metabolomics and proteomics studies, focusing on the analysis of stress response markers. <i>Cs</i>RALF34 overexpression resulted in the inhibition of root growth and regulation of cell proliferation, specifically in blocking the G2/M transition in cucumber roots. Based on these results, we propose that <i>CsRALF34</i> is not part of the gene regulatory networks involved in the early steps of lateral root initiation. Instead, we suggest that <i>Cs</i>RALF34 modulates ROS homeostasis and triggers the controlled production of hydroxyl radicals in root cells, possibly associated with intracellular signal transduction. Altogether, our results support the role of RALF peptides as ROS regulators.https://www.mdpi.com/1422-0067/24/8/7654<i>Cucumis sativus</i> (cucumber)small signaling peptidesRALF34root developmentoverexpressionhairy root transformation |
| spellingShingle | Julia Shumilina Alexey S. Kiryushkin Nadezhda Frolova Valeria Mashkina Elena L. Ilina Vera A. Puchkova Katerina Danko Svetlana Silinskaya Evgeny B. Serebryakov Alena Soboleva Tatiana Bilova Anastasia Orlova Elizaveta D. Guseva Egor Repkin Katharina Pawlowski Andrej Frolov Kirill N. Demchenko Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots International Journal of Molecular Sciences <i>Cucumis sativus</i> (cucumber) small signaling peptides RALF34 root development overexpression hairy root transformation |
| title | Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots |
| title_full | Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots |
| title_fullStr | Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots |
| title_full_unstemmed | Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots |
| title_short | Integrative Proteomics and Metabolomics Analysis Reveals the Role of Small Signaling Peptide Rapid Alkalinization Factor 34 (RALF34) in Cucumber Roots |
| title_sort | integrative proteomics and metabolomics analysis reveals the role of small signaling peptide rapid alkalinization factor 34 ralf34 in cucumber roots |
| topic | <i>Cucumis sativus</i> (cucumber) small signaling peptides RALF34 root development overexpression hairy root transformation |
| url | https://www.mdpi.com/1422-0067/24/8/7654 |
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