Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport Conditions
This study investigated the effects of added eugenol on water quality and the metabolism and antioxidant capacity of the liver and gills of the greater amberjack (<i>Seriola dumerili</i>) during simulated transport. The juvenile fish (10.34 ± 1.33 g) were transported in sealed plastic ba...
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MDPI AG
2022-10-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/20/2880 |
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| author | Yuhang He Zhengyi Fu Shiming Dai Gang Yu Yunfeng Guo Zhenhua Ma |
| author_facet | Yuhang He Zhengyi Fu Shiming Dai Gang Yu Yunfeng Guo Zhenhua Ma |
| author_sort | Yuhang He |
| collection | DOAJ |
| description | This study investigated the effects of added eugenol on water quality and the metabolism and antioxidant capacity of the liver and gills of the greater amberjack (<i>Seriola dumerili</i>) during simulated transport. The juvenile fish (10.34 ± 1.33 g) were transported in sealed plastic bags containing different eugenol concentrations at a density of 24.79 kg/m<sup>3</sup> for 8 h. The different eugenol concentrations were divided into five groups: 0 μL/mL (control group), 0.0125 μL/mL, 0.025 μL/mL, 0.0375 μL/mL, and 0.05 μL/mL, with three replicates of each. The results showed that 0.05 μL/mL of eugenol could significantly increase dissolved oxygen, but 0.025 μL/mL–0.0375 μL/mL resulted in a significant decrease in dissolved oxygen and significant increases in NH<sub>4</sub><sup>+</sup>-N and NO<sub>2</sub><sup>−</sup>-N. It was found that 0.05 μL/mL of eugenol caused significant up-regulation of the relative expression of CPT-1 in the liver, significant down-regulation of the relative expression of FAS and PK in the liver and gills, a significant increase in glycogen concentration, and a significant decrease in glucose concentration. This suggests that 0.05 μL/mL of eugenol could reduce the metabolic capacity of fish. In addition, 0.05 μL/mL of eugenol caused significant up-regulation of the relative expression of CAT and a significant decrease of MDA concentration in the liver. Meanwhile, the gills showed significant up-regulation of CAT relative expression, significant down-regulation of Keap1 relative expression, and a significant increase in GSH activity, resulting in a significant increase in MDA concentration when the concentration of eugenol reached or exceeded 0.025 μL/mL. This suggests that 0.05 μL/mL eugenol could improve the antioxidant capacity of fish and lipid peroxidation levels in the gills. In conclusion, the addition of 0.05 μL/mL eugenol could improve water quality, and the metabolic and antioxidant capacities of liver and gills, but it could also increase lipid peroxidation levels in the gills under transport conditions. |
| first_indexed | 2024-03-09T20:51:32Z |
| format | Article |
| id | doaj.art-601208c4bfee44ccbbf04f05e4b8dd92 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T20:51:32Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-601208c4bfee44ccbbf04f05e4b8dd922023-11-23T22:33:30ZengMDPI AGAnimals2076-26152022-10-011220288010.3390/ani12202880Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport ConditionsYuhang He0Zhengyi Fu1Shiming Dai2Gang Yu3Yunfeng Guo4Zhenhua Ma5Sanya Tropical Fisheries Research Institute, Sanya 572018, ChinaSanya Tropical Fisheries Research Institute, Sanya 572018, ChinaSanya Tropical Fisheries Research Institute, Sanya 572018, ChinaSanya Tropical Fisheries Research Institute, Sanya 572018, ChinaFisheries Management and Law Enforcement Service Centre, Ministry of Agriculture and Rural Affairs, Shanghai 200092, ChinaSanya Tropical Fisheries Research Institute, Sanya 572018, ChinaThis study investigated the effects of added eugenol on water quality and the metabolism and antioxidant capacity of the liver and gills of the greater amberjack (<i>Seriola dumerili</i>) during simulated transport. The juvenile fish (10.34 ± 1.33 g) were transported in sealed plastic bags containing different eugenol concentrations at a density of 24.79 kg/m<sup>3</sup> for 8 h. The different eugenol concentrations were divided into five groups: 0 μL/mL (control group), 0.0125 μL/mL, 0.025 μL/mL, 0.0375 μL/mL, and 0.05 μL/mL, with three replicates of each. The results showed that 0.05 μL/mL of eugenol could significantly increase dissolved oxygen, but 0.025 μL/mL–0.0375 μL/mL resulted in a significant decrease in dissolved oxygen and significant increases in NH<sub>4</sub><sup>+</sup>-N and NO<sub>2</sub><sup>−</sup>-N. It was found that 0.05 μL/mL of eugenol caused significant up-regulation of the relative expression of CPT-1 in the liver, significant down-regulation of the relative expression of FAS and PK in the liver and gills, a significant increase in glycogen concentration, and a significant decrease in glucose concentration. This suggests that 0.05 μL/mL of eugenol could reduce the metabolic capacity of fish. In addition, 0.05 μL/mL of eugenol caused significant up-regulation of the relative expression of CAT and a significant decrease of MDA concentration in the liver. Meanwhile, the gills showed significant up-regulation of CAT relative expression, significant down-regulation of Keap1 relative expression, and a significant increase in GSH activity, resulting in a significant increase in MDA concentration when the concentration of eugenol reached or exceeded 0.025 μL/mL. This suggests that 0.05 μL/mL eugenol could improve the antioxidant capacity of fish and lipid peroxidation levels in the gills. In conclusion, the addition of 0.05 μL/mL eugenol could improve water quality, and the metabolic and antioxidant capacities of liver and gills, but it could also increase lipid peroxidation levels in the gills under transport conditions.https://www.mdpi.com/2076-2615/12/20/2880Eugenollivergillmetabolism capacityantioxidant capacitywater quality |
| spellingShingle | Yuhang He Zhengyi Fu Shiming Dai Gang Yu Yunfeng Guo Zhenhua Ma Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport Conditions Animals Eugenol liver gill metabolism capacity antioxidant capacity water quality |
| title | Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport Conditions |
| title_full | Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport Conditions |
| title_fullStr | Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport Conditions |
| title_full_unstemmed | Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport Conditions |
| title_short | Effects of Eugenol on Water Quality and the Metabolism and Antioxidant Capacity of Juvenile Greater Amberjack (<i>Seriola dumerili</i>) under Simulated Transport Conditions |
| title_sort | effects of eugenol on water quality and the metabolism and antioxidant capacity of juvenile greater amberjack i seriola dumerili i under simulated transport conditions |
| topic | Eugenol liver gill metabolism capacity antioxidant capacity water quality |
| url | https://www.mdpi.com/2076-2615/12/20/2880 |
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