Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental Infection
The development of an effective vaccine against leishmaniasis is the aim of an intensive research effort, to bring relief to thousands of people worldwide. DNA vaccination is a promising approach in this direction, since it is able to generate a strong cellular immune response. We tested whether the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2004-05-01
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| Series: | European Journal of Inflammation |
| Online Access: | https://doi.org/10.1177/1721727X0400200207 |
| _version_ | 1811337832278523904 |
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| author | P. Tsagozis V. Tseveleki L. Probert E. Dotsika E. Karagouni |
| author_facet | P. Tsagozis V. Tseveleki L. Probert E. Dotsika E. Karagouni |
| author_sort | P. Tsagozis |
| collection | DOAJ |
| description | The development of an effective vaccine against leishmaniasis is the aim of an intensive research effort, to bring relief to thousands of people worldwide. DNA vaccination is a promising approach in this direction, since it is able to generate a strong cellular immune response. We tested whether the co-administration of a plasmid encoding a truncated gp63 gene, that allows extracellular secretion of the encoded protein, and a plasmid encoding for CD40L could induce a protective response in genetically susceptible BALB/c mice and reduce the local inflammatory swelling after infection with Leishmania major . We document that vaccination with the combination of plasmids for gp63 and CD40L reduced inflammatory swelling, while vaccination with the truncated gp63-encoding plasmid resulted in an exacerbation of the local inflammatory reaction. These preliminary data indicate that the CD40L expression plasmid is consequently an efficient adjuvant for the induction of protective responses in the context of a DNA vaccination against leishmaniasis. |
| first_indexed | 2024-04-13T18:01:40Z |
| format | Article |
| id | doaj.art-60129782893d41c29e895d4c1547316f |
| institution | Directory Open Access Journal |
| issn | 1721-727X |
| language | English |
| last_indexed | 2024-04-13T18:01:40Z |
| publishDate | 2004-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | European Journal of Inflammation |
| spelling | doaj.art-60129782893d41c29e895d4c1547316f2022-12-22T02:36:14ZengSAGE PublishingEuropean Journal of Inflammation1721-727X2004-05-01210.1177/1721727X0400200207Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental InfectionP. TsagozisV. Tseveleki0L. Probert1E. DotsikaE. Karagouni Laboratory of Molecular Genetics, Institut Pasteur Hellenique, Athens, Greece Laboratory of Molecular Genetics, Institut Pasteur Hellenique, Athens, GreeceThe development of an effective vaccine against leishmaniasis is the aim of an intensive research effort, to bring relief to thousands of people worldwide. DNA vaccination is a promising approach in this direction, since it is able to generate a strong cellular immune response. We tested whether the co-administration of a plasmid encoding a truncated gp63 gene, that allows extracellular secretion of the encoded protein, and a plasmid encoding for CD40L could induce a protective response in genetically susceptible BALB/c mice and reduce the local inflammatory swelling after infection with Leishmania major . We document that vaccination with the combination of plasmids for gp63 and CD40L reduced inflammatory swelling, while vaccination with the truncated gp63-encoding plasmid resulted in an exacerbation of the local inflammatory reaction. These preliminary data indicate that the CD40L expression plasmid is consequently an efficient adjuvant for the induction of protective responses in the context of a DNA vaccination against leishmaniasis.https://doi.org/10.1177/1721727X0400200207 |
| spellingShingle | P. Tsagozis V. Tseveleki L. Probert E. Dotsika E. Karagouni Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental Infection European Journal of Inflammation |
| title | Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental Infection |
| title_full | Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental Infection |
| title_fullStr | Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental Infection |
| title_full_unstemmed | Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental Infection |
| title_short | Vaccination with Plasmids Encoding the GP63 Glycoprotein and CD40L Results in a Partial Suppression of the Inflammatory Reaction after Experimental Infection |
| title_sort | vaccination with plasmids encoding the gp63 glycoprotein and cd40l results in a partial suppression of the inflammatory reaction after experimental infection |
| url | https://doi.org/10.1177/1721727X0400200207 |
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