Heteroalleles in Common Wheat: Multiple Differences between Allelic Variants of the <i>Gli-B1</i> Locus
The <i>Gli-B1</i>-encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patt...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/4/1832 |
| Summary: | The <i>Gli-B1</i>-encoded γ-gliadins and non-coding γ-gliadin DNA sequences for 15 different alleles of common wheat have been compared using seven tests: electrophoretic mobility (EM) and molecular weight (MW) of the encoded major γ-gliadin, restriction fragment length polymorphism patterns (RFLPs) (three different markers), <i>Gli-B1</i>-γ-gliadin-pseudogene known SNP markers (Single nucleotide polymorphisms) and sequencing the pseudogene <i>GAG56B</i>. It was discovered that encoded γ-gliadins, with contrasting EM, had similar MWs. However, seven allelic variants (designated from I to VII) differed among them in the other six tests: I (alleles <i>Gli-B1i</i>, <i>k</i>, <i>m</i>, <i>o</i>), II (<i>Gli-B1n</i>, <i>q</i>, <i>s</i>), III (<i>Gli-B1b</i>), IV (<i>Gli-B1e</i>, <i>f</i>, <i>g</i>), V (<i>Gli-B1h</i>), VI (<i>Gli-B1d</i>) and VII (<i>Gli-B1a</i>). Allele <i>Gli-B1c</i> (variant VIII) was identical to the alleles from group IV in four of the tests. Some tests might show a fine difference between alleles belonging to the same variant. Our results attest in favor of the independent origin of at least seven variants at the <i>Gli-B1</i> locus that might originate from deeply diverged genotypes of the donor(s) of the B genome in hexaploid wheat and therefore might be called “heteroallelic”. The donor’s particularities at the <i>Gli-B1</i> locus might be conserved since that time and decisively contribute to the current high genetic diversity of common wheat. |
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| ISSN: | 1661-6596 1422-0067 |