Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout. Moderate to severe dry eye has been observed in the patients infected with COVID-19. Material and Method: We...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2022-01-01
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| Series: | Medical Journal of Dr. D.Y. Patil Vidyapeeth |
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| Online Access: | http://www.mjdrdypv.org/article.asp?issn=2589-8302;year=2022;volume=15;issue=7;spage=46;epage=48;aulast=Mishra |
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| author | Neeta Mishra Chandrashekhar Raut Neelam Prasad J S Bhawalkar Safia Farooqui Shahzad Mirza Veshal V Madan |
| author_facet | Neeta Mishra Chandrashekhar Raut Neelam Prasad J S Bhawalkar Safia Farooqui Shahzad Mirza Veshal V Madan |
| author_sort | Neeta Mishra |
| collection | DOAJ |
| description | Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout. Moderate to severe dry eye has been observed in the patients infected with COVID-19. Material and Method: We conducted a descriptive, prospective observational study. We enrolled 16 patients fulfilling the inclusion criteria for our study. Patients affected with acute COVID-19 from 28-9-2020 to 9-10-2020 registered at our hospital and willing to participate were included. Tears and conjunctival swabs were taken at the interval of 24 h; consecutively three samples were taken from one COVID-19 infected patient. In total, 48 samples were analyzed by qRT-PCR. Result: We had 16 participants, 47% were females and 53% were males; 2/16 [12.5%] patients showed the presence of SARS-CoV-2 in tears and conjunctival swabs. Both the infected patients were females, and they did not have any obvious abnormal ocular conditions. Schirmer strip reading, however, showed a severe dry eye in both the patients. Conclusion: We identified dry eye ranging from mild to moderate to severe [Schirmer 1 reading ranged from 2 to 8 mm] in all our patients admitted to the COVID-19 ward. The SARS-CoV-2 gene seems to be mutating. More work needs to be done to find the viral load in the tears and any abnormalities in the eyes accordingly. |
| first_indexed | 2024-04-11T21:27:36Z |
| format | Article |
| id | doaj.art-6012faf795ed43feba239c12821bf917 |
| institution | Directory Open Access Journal |
| issn | 2589-8302 2589-8310 |
| language | English |
| last_indexed | 2024-04-11T21:27:36Z |
| publishDate | 2022-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Dr. D.Y. Patil Vidyapeeth |
| spelling | doaj.art-6012faf795ed43feba239c12821bf9172022-12-22T04:02:19ZengWolters Kluwer Medknow PublicationsMedical Journal of Dr. D.Y. Patil Vidyapeeth2589-83022589-83102022-01-01157464810.4103/mjdrdypu.mjdrdypu_708_20Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)Neeta MishraChandrashekhar RautNeelam PrasadJ S BhawalkarSafia FarooquiShahzad MirzaVeshal V MadanBackground: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout. Moderate to severe dry eye has been observed in the patients infected with COVID-19. Material and Method: We conducted a descriptive, prospective observational study. We enrolled 16 patients fulfilling the inclusion criteria for our study. Patients affected with acute COVID-19 from 28-9-2020 to 9-10-2020 registered at our hospital and willing to participate were included. Tears and conjunctival swabs were taken at the interval of 24 h; consecutively three samples were taken from one COVID-19 infected patient. In total, 48 samples were analyzed by qRT-PCR. Result: We had 16 participants, 47% were females and 53% were males; 2/16 [12.5%] patients showed the presence of SARS-CoV-2 in tears and conjunctival swabs. Both the infected patients were females, and they did not have any obvious abnormal ocular conditions. Schirmer strip reading, however, showed a severe dry eye in both the patients. Conclusion: We identified dry eye ranging from mild to moderate to severe [Schirmer 1 reading ranged from 2 to 8 mm] in all our patients admitted to the COVID-19 ward. The SARS-CoV-2 gene seems to be mutating. More work needs to be done to find the viral load in the tears and any abnormalities in the eyes accordingly.http://www.mjdrdypv.org/article.asp?issn=2589-8302;year=2022;volume=15;issue=7;spage=46;epage=48;aulast=Mishracovid -19qrt-pcrschirmer strip |
| spellingShingle | Neeta Mishra Chandrashekhar Raut Neelam Prasad J S Bhawalkar Safia Farooqui Shahzad Mirza Veshal V Madan Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR) Medical Journal of Dr. D.Y. Patil Vidyapeeth covid -19 qrt-pcr schirmer strip |
| title | Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR) |
| title_full | Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR) |
| title_fullStr | Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR) |
| title_full_unstemmed | Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR) |
| title_short | Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR) |
| title_sort | presence of sars cov 2 in human tears detected by quantitative real time pcr qrt pcr |
| topic | covid -19 qrt-pcr schirmer strip |
| url | http://www.mjdrdypv.org/article.asp?issn=2589-8302;year=2022;volume=15;issue=7;spage=46;epage=48;aulast=Mishra |
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