Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain Barriers
The effect of food components on brain growth and development has attracted increasing attention. Milk has been shown to contain peptides that deliver important signals to the brains of neonates and infants. In order to reach the brain, milk peptides have to resist proteolytic degradation in the gas...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/10/3157 |
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| author | Brian Christensen Andrea E. Toth Simone S. E. Nielsen Carsten Scavenius Steen V. Petersen Jan J. Enghild Jan T. Rasmussen Morten S. Nielsen Esben S. Sørensen |
| author_facet | Brian Christensen Andrea E. Toth Simone S. E. Nielsen Carsten Scavenius Steen V. Petersen Jan J. Enghild Jan T. Rasmussen Morten S. Nielsen Esben S. Sørensen |
| author_sort | Brian Christensen |
| collection | DOAJ |
| description | The effect of food components on brain growth and development has attracted increasing attention. Milk has been shown to contain peptides that deliver important signals to the brains of neonates and infants. In order to reach the brain, milk peptides have to resist proteolytic degradation in the gastrointestinal tract, cross the gastrointestinal barrier and later cross the highly selective blood–brain barrier (BBB). To investigate this, we purified and characterized endogenous peptides from bovine milk and investigated their apical to basal transport by using human intestinal Caco-2 cells and primary porcine brain endothelial cell monolayer models. Among 192 characterized milk peptides, only the α<sub>S1</sub>-casein peptide <sup>185</sup>PIGSENSEKTTMPLW<sup>199</sup>, and especially fragments of this peptide processed during the transport, could cross both the intestinal barrier and the BBB cell monolayer models. This peptide was also shown to resist simulated gastrointestinal digestion. This study demonstrates that a milk derived peptide can cross the major biological barriers in vitro and potentially reach the brain, where it may deliver physiological signals. |
| first_indexed | 2024-03-10T15:34:28Z |
| format | Article |
| id | doaj.art-6013432a09cc464f983220d7edfad8c0 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T15:34:28Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-6013432a09cc464f983220d7edfad8c02023-11-20T17:19:15ZengMDPI AGNutrients2072-66432020-10-011210315710.3390/nu12103157Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain BarriersBrian Christensen0Andrea E. Toth1Simone S. E. Nielsen2Carsten Scavenius3Steen V. Petersen4Jan J. Enghild5Jan T. Rasmussen6Morten S. Nielsen7Esben S. Sørensen8Department of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Biomedicine, Faculty of Health, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Biomedicine, Faculty of Health, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Biomedicine, Faculty of Health, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Biomedicine, Faculty of Health, Aarhus University, DK-8000 Aarhus, DenmarkDepartment of Molecular Biology and Genetics, Aarhus University, DK-8000 Aarhus, DenmarkThe effect of food components on brain growth and development has attracted increasing attention. Milk has been shown to contain peptides that deliver important signals to the brains of neonates and infants. In order to reach the brain, milk peptides have to resist proteolytic degradation in the gastrointestinal tract, cross the gastrointestinal barrier and later cross the highly selective blood–brain barrier (BBB). To investigate this, we purified and characterized endogenous peptides from bovine milk and investigated their apical to basal transport by using human intestinal Caco-2 cells and primary porcine brain endothelial cell monolayer models. Among 192 characterized milk peptides, only the α<sub>S1</sub>-casein peptide <sup>185</sup>PIGSENSEKTTMPLW<sup>199</sup>, and especially fragments of this peptide processed during the transport, could cross both the intestinal barrier and the BBB cell monolayer models. This peptide was also shown to resist simulated gastrointestinal digestion. This study demonstrates that a milk derived peptide can cross the major biological barriers in vitro and potentially reach the brain, where it may deliver physiological signals.https://www.mdpi.com/2072-6643/12/10/3157milk peptidesgastrointestinal digestionCaco-2 cellsblood–brain barrierpeptide transport |
| spellingShingle | Brian Christensen Andrea E. Toth Simone S. E. Nielsen Carsten Scavenius Steen V. Petersen Jan J. Enghild Jan T. Rasmussen Morten S. Nielsen Esben S. Sørensen Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain Barriers Nutrients milk peptides gastrointestinal digestion Caco-2 cells blood–brain barrier peptide transport |
| title | Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain Barriers |
| title_full | Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain Barriers |
| title_fullStr | Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain Barriers |
| title_full_unstemmed | Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain Barriers |
| title_short | Transport of a Peptide from Bovine α<sub>s1</sub>-Casein across Models of the Intestinal and Blood–Brain Barriers |
| title_sort | transport of a peptide from bovine α sub s1 sub casein across models of the intestinal and blood brain barriers |
| topic | milk peptides gastrointestinal digestion Caco-2 cells blood–brain barrier peptide transport |
| url | https://www.mdpi.com/2072-6643/12/10/3157 |
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