Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization
Photo-polymerized hydrogels are ideally suited for stem-cell based tissue regeneration and three dimensional (3D) bioprinting because they can be highly biocompatible, injectable, easy to use, and their mechanical and physical properties can be controlled. However, photo-polymerization involves the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/11/6095 |
| _version_ | 1797531237089280000 |
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| author | Silvia Buonvino Matteo Ciocci Dror Seliktar Sonia Melino |
| author_facet | Silvia Buonvino Matteo Ciocci Dror Seliktar Sonia Melino |
| author_sort | Silvia Buonvino |
| collection | DOAJ |
| description | Photo-polymerized hydrogels are ideally suited for stem-cell based tissue regeneration and three dimensional (3D) bioprinting because they can be highly biocompatible, injectable, easy to use, and their mechanical and physical properties can be controlled. However, photo-polymerization involves the use of potentially toxic photo-initiators, exposure to ultraviolet light radiation, formation of free radicals that trigger the cross-linking reaction, and other events whose effects on cells are not yet fully understood. The purpose of this study was to examine the effects of hydrogen sulfide (H<sub>2</sub>S) in mitigating cellular toxicity of photo-polymerization caused to resident cells during the process of hydrogel formation. H<sub>2</sub>S, which is the latest discovered member of the <i>gasotransmitter</i> family of gaseous signalling molecules, has a number of established beneficial properties, including cell protection from oxidative damage both directly (by acting as a scavenger molecule) and indirectly (by inducing the expression of anti-oxidant proteins in the cell). Cells were exposed to slow release H<sub>2</sub>S treatment using pre-conditioning with glutathione-conjugated-garlic extract in order to mitigate toxicity during the photo-polymerization process of hydrogel formation. The protective effects of the H<sub>2</sub>S treatment were evaluated in both an enzymatic model and a 3D cell culture system using cell viability as a quantitative indicator. The protective effect of H<sub>2</sub>S treatment of cells is a promising approach to enhance cell survival in tissue engineering applications requiring photo-polymerized hydrogel scaffolds. |
| first_indexed | 2024-03-10T10:40:59Z |
| format | Article |
| id | doaj.art-60137b7e973a497184ea1b9b32dcc43e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:40:59Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-60137b7e973a497184ea1b9b32dcc43e2023-11-21T22:55:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012211609510.3390/ijms22116095Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems OptimizationSilvia Buonvino0Matteo Ciocci1Dror Seliktar2Sonia Melino3Department of Chemical Science and Technologies, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Chemical Science and Technologies, University of Rome “Tor Vergata”, 00133 Rome, ItalyDepartment of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa 3200003, IsraelDepartment of Chemical Science and Technologies, University of Rome “Tor Vergata”, 00133 Rome, ItalyPhoto-polymerized hydrogels are ideally suited for stem-cell based tissue regeneration and three dimensional (3D) bioprinting because they can be highly biocompatible, injectable, easy to use, and their mechanical and physical properties can be controlled. However, photo-polymerization involves the use of potentially toxic photo-initiators, exposure to ultraviolet light radiation, formation of free radicals that trigger the cross-linking reaction, and other events whose effects on cells are not yet fully understood. The purpose of this study was to examine the effects of hydrogen sulfide (H<sub>2</sub>S) in mitigating cellular toxicity of photo-polymerization caused to resident cells during the process of hydrogel formation. H<sub>2</sub>S, which is the latest discovered member of the <i>gasotransmitter</i> family of gaseous signalling molecules, has a number of established beneficial properties, including cell protection from oxidative damage both directly (by acting as a scavenger molecule) and indirectly (by inducing the expression of anti-oxidant proteins in the cell). Cells were exposed to slow release H<sub>2</sub>S treatment using pre-conditioning with glutathione-conjugated-garlic extract in order to mitigate toxicity during the photo-polymerization process of hydrogel formation. The protective effects of the H<sub>2</sub>S treatment were evaluated in both an enzymatic model and a 3D cell culture system using cell viability as a quantitative indicator. The protective effect of H<sub>2</sub>S treatment of cells is a promising approach to enhance cell survival in tissue engineering applications requiring photo-polymerized hydrogel scaffolds.https://www.mdpi.com/1422-0067/22/11/6095photo-polymerizationprotein hydrogeltissue repairstem cellsenzymeradicals |
| spellingShingle | Silvia Buonvino Matteo Ciocci Dror Seliktar Sonia Melino Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization International Journal of Molecular Sciences photo-polymerization protein hydrogel tissue repair stem cells enzyme radicals |
| title | Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization |
| title_full | Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization |
| title_fullStr | Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization |
| title_full_unstemmed | Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization |
| title_short | Photo-Polymerization Damage Protection by Hydrogen Sulfide Donors for 3D-Cell Culture Systems Optimization |
| title_sort | photo polymerization damage protection by hydrogen sulfide donors for 3d cell culture systems optimization |
| topic | photo-polymerization protein hydrogel tissue repair stem cells enzyme radicals |
| url | https://www.mdpi.com/1422-0067/22/11/6095 |
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