Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitans
Abstract Apoptosis‐like programmed cell death is associated with fungal development, ageing, pathogenicity and stress responses. Here, to explore the potential of Botrytis cinerea type II inhibitor of apoptosis (IAP) BcBIR1 in elevating the biocontrol efficacy of Coniothyrium minitans, the BcBIR1 ge...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-02-01
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| Series: | Microbial Biotechnology |
| Online Access: | https://doi.org/10.1111/1751-7915.14402 |
| _version_ | 1797294796155387904 |
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| author | Jianing Wu Ruolong Xin Yachan Jiang Huanan Jin Hao Liu Hongxiang Zhang Daohong Jiang Yanping Fu Jiatao Xie Jiasen Cheng Yang Lin |
| author_facet | Jianing Wu Ruolong Xin Yachan Jiang Huanan Jin Hao Liu Hongxiang Zhang Daohong Jiang Yanping Fu Jiatao Xie Jiasen Cheng Yang Lin |
| author_sort | Jianing Wu |
| collection | DOAJ |
| description | Abstract Apoptosis‐like programmed cell death is associated with fungal development, ageing, pathogenicity and stress responses. Here, to explore the potential of Botrytis cinerea type II inhibitor of apoptosis (IAP) BcBIR1 in elevating the biocontrol efficacy of Coniothyrium minitans, the BcBIR1 gene was heterologously expressed in C. minitans. Results indicated that the strains expressing BcBIR1 had higher rates of conidiation, mycelial growth and biomass growth than the wild‐type strain. Moreover, BcBIR1 was found to inhibit apoptosis, indicating its role as an IAP in C. minitans. Under various abiotic stresses, the growth rates of BcBIR1‐expressing strains were significantly higher than that of the wild‐type strain. Moreover, the conidial survival rate of the BcBIR1‐expressing strains treated with ultraviolet irradiation was enhanced. In antifungal activity assay, the culture filtrates of BcBIR1‐expressing strains displayed a stronger inhibitory effect on B. cinerea and Sclerotinia sclerotiorum than the wild‐type strain. The study also found that BcBIR1 expression increased the mycoparasitism against the sclerotia, but not the hyphae of S. sclerotiorum. Taken together, these results suggest that BcBIR1 enhances vegetative growth, conidiation, anti‐apoptosis activity, abiotic stress resistance, antifungal activity and mycoparasitism in C. minitans. As an IAP, BcBIR1 may improve the control capacity of C. minitans against S. sclerotiorum. |
| first_indexed | 2024-03-07T21:36:32Z |
| format | Article |
| id | doaj.art-6014290873844627a6c9801cc256969b |
| institution | Directory Open Access Journal |
| issn | 1751-7915 |
| language | English |
| last_indexed | 2024-03-07T21:36:32Z |
| publishDate | 2024-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Microbial Biotechnology |
| spelling | doaj.art-6014290873844627a6c9801cc256969b2024-02-26T12:12:42ZengWileyMicrobial Biotechnology1751-79152024-02-01172n/an/a10.1111/1751-7915.14402Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitansJianing Wu0Ruolong Xin1Yachan Jiang2Huanan Jin3Hao Liu4Hongxiang Zhang5Daohong Jiang6Yanping Fu7Jiatao Xie8Jiasen Cheng9Yang Lin10State Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology Huazhong Agricultural University Wuhan ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology Huazhong Agricultural University Wuhan ChinaKey Laboratory of Elemene Class Anti‐cancer Chinese Medicine of Zhejiang Province, Engineering Laboratory of Development and Application of Traditional Chinese Medicine from Zhejiang Province, Holistic Integrative Pharmacy Institute, Health Science Center Hangzhou Normal University Hangzhou Zhejiang ChinaState Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan ChinaState Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan ChinaState Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology Huazhong Agricultural University Wuhan ChinaState Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan ChinaState Key Laboratory of Agricultural Microbiology Huazhong Agricultural University Wuhan ChinaThe Provincial Key Lab of Plant Pathology of Hubei Province, College of Plant Science and Technology Huazhong Agricultural University Wuhan ChinaAbstract Apoptosis‐like programmed cell death is associated with fungal development, ageing, pathogenicity and stress responses. Here, to explore the potential of Botrytis cinerea type II inhibitor of apoptosis (IAP) BcBIR1 in elevating the biocontrol efficacy of Coniothyrium minitans, the BcBIR1 gene was heterologously expressed in C. minitans. Results indicated that the strains expressing BcBIR1 had higher rates of conidiation, mycelial growth and biomass growth than the wild‐type strain. Moreover, BcBIR1 was found to inhibit apoptosis, indicating its role as an IAP in C. minitans. Under various abiotic stresses, the growth rates of BcBIR1‐expressing strains were significantly higher than that of the wild‐type strain. Moreover, the conidial survival rate of the BcBIR1‐expressing strains treated with ultraviolet irradiation was enhanced. In antifungal activity assay, the culture filtrates of BcBIR1‐expressing strains displayed a stronger inhibitory effect on B. cinerea and Sclerotinia sclerotiorum than the wild‐type strain. The study also found that BcBIR1 expression increased the mycoparasitism against the sclerotia, but not the hyphae of S. sclerotiorum. Taken together, these results suggest that BcBIR1 enhances vegetative growth, conidiation, anti‐apoptosis activity, abiotic stress resistance, antifungal activity and mycoparasitism in C. minitans. As an IAP, BcBIR1 may improve the control capacity of C. minitans against S. sclerotiorum.https://doi.org/10.1111/1751-7915.14402 |
| spellingShingle | Jianing Wu Ruolong Xin Yachan Jiang Huanan Jin Hao Liu Hongxiang Zhang Daohong Jiang Yanping Fu Jiatao Xie Jiasen Cheng Yang Lin Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitans Microbial Biotechnology |
| title | Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitans |
| title_full | Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitans |
| title_fullStr | Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitans |
| title_full_unstemmed | Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitans |
| title_short | Botrytis cinerea type II inhibitor of apoptosis BcBIR1 enhances the biocontrol capacity of Coniothyrium minitans |
| title_sort | botrytis cinerea type ii inhibitor of apoptosis bcbir1 enhances the biocontrol capacity of coniothyrium minitans |
| url | https://doi.org/10.1111/1751-7915.14402 |
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