Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar Rats
Introduction: The present study investigated the role of the Basolateral Amygdala (BLA) N-methyl-D-aspartate (NMDA) receptors in stress-induced spatial memory disturbance among the male Wistar rats. Methods: The male Wistar rats (Average weight =200 g) were cannulated bilaterally in the BLA, and en...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Iran University of Medical Sciences
2020-07-01
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| Series: | Basic and Clinical Neuroscience |
| Subjects: | |
| Online Access: | http://bcn.iums.ac.ir/article-1-1497-en.html |
| _version_ | 1797280578346680320 |
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| author | Ahmad Rafia Shahrbanoo Oryan Akram Eidi Hedayat Sahraei |
| author_facet | Ahmad Rafia Shahrbanoo Oryan Akram Eidi Hedayat Sahraei |
| author_sort | Ahmad Rafia |
| collection | DOAJ |
| description | Introduction: The present study investigated the role of the Basolateral Amygdala (BLA) N-methyl-D-aspartate (NMDA) receptors in stress-induced spatial memory disturbance among the male Wistar rats.
Methods: The male Wistar rats (Average weight =200 g) were cannulated bilaterally in the BLA, and entered the study (n=6-8) after one week. They received seven electro–foot-shock stress sessions on seven consecutive days. Memantine (0.1, 1, and 5 µg/rat) or saline (0.5 µL/rat) was injected into the BLA, five minutes before each stress session. The control groups received the same doses of memantine and no stress. After the end of the stress sessions, blood samples were taken from all animals to evaluate their plasma corticosterone. Also, the spatial learning and memory of the study animals were evaluated using the Barnes maze method. The animals experienced five consecutive days of training on the maze for spatial learning. On the sixth day, their spatial memory was evaluated on the maze. Time, distance, the number of errors, and the taking strategy for reaching the target hole were considered as the parameters for the spatial learning and memory evaluation.
Results: Stress increases the plasma corticosterone level, while memantine preadministration reduces the stress effects. Besides, stress increases the time and distance to the target hole and the number of errors. Stress changed the animals’ strategy from serial to random type. However, the intra-BLA memantine reversed all the disturbances induced by the stress.
Conclusion: This study indicated that the BLA glutamate NMDA receptors modulate the effect of stress on spatial learning and memory deficit. |
| first_indexed | 2024-03-07T16:43:15Z |
| format | Article |
| id | doaj.art-6015abf0c15e4ca9a1f6a712747d0577 |
| institution | Directory Open Access Journal |
| issn | 2008-126X 2228-7442 |
| language | English |
| last_indexed | 2024-03-07T16:43:15Z |
| publishDate | 2020-07-01 |
| publisher | Iran University of Medical Sciences |
| record_format | Article |
| series | Basic and Clinical Neuroscience |
| spelling | doaj.art-6015abf0c15e4ca9a1f6a712747d05772024-03-03T07:15:32ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422020-07-01114447456Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar RatsAhmad Rafia0Shahrbanoo Oryan1Akram Eidi2Hedayat Sahraei3 Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Tehran, Iran. Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Tehran, Iran. Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Tehran, Iran. Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. Introduction: The present study investigated the role of the Basolateral Amygdala (BLA) N-methyl-D-aspartate (NMDA) receptors in stress-induced spatial memory disturbance among the male Wistar rats. Methods: The male Wistar rats (Average weight =200 g) were cannulated bilaterally in the BLA, and entered the study (n=6-8) after one week. They received seven electro–foot-shock stress sessions on seven consecutive days. Memantine (0.1, 1, and 5 µg/rat) or saline (0.5 µL/rat) was injected into the BLA, five minutes before each stress session. The control groups received the same doses of memantine and no stress. After the end of the stress sessions, blood samples were taken from all animals to evaluate their plasma corticosterone. Also, the spatial learning and memory of the study animals were evaluated using the Barnes maze method. The animals experienced five consecutive days of training on the maze for spatial learning. On the sixth day, their spatial memory was evaluated on the maze. Time, distance, the number of errors, and the taking strategy for reaching the target hole were considered as the parameters for the spatial learning and memory evaluation. Results: Stress increases the plasma corticosterone level, while memantine preadministration reduces the stress effects. Besides, stress increases the time and distance to the target hole and the number of errors. Stress changed the animals’ strategy from serial to random type. However, the intra-BLA memantine reversed all the disturbances induced by the stress. Conclusion: This study indicated that the BLA glutamate NMDA receptors modulate the effect of stress on spatial learning and memory deficit.http://bcn.iums.ac.ir/article-1-1497-en.htmlbasolateral amygdalaglutamate nmda receptorsmemantinespatial learning and memorystress |
| spellingShingle | Ahmad Rafia Shahrbanoo Oryan Akram Eidi Hedayat Sahraei Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar Rats Basic and Clinical Neuroscience basolateral amygdala glutamate nmda receptors memantine spatial learning and memory stress |
| title | Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar Rats |
| title_full | Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar Rats |
| title_fullStr | Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar Rats |
| title_full_unstemmed | Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar Rats |
| title_short | Stress-Induced Spatial Memory Deficit Reversed by Basolateral Amygdala NMDA Receptor Inhibition in Male Wistar Rats |
| title_sort | stress induced spatial memory deficit reversed by basolateral amygdala nmda receptor inhibition in male wistar rats |
| topic | basolateral amygdala glutamate nmda receptors memantine spatial learning and memory stress |
| url | http://bcn.iums.ac.ir/article-1-1497-en.html |
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