Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats
Inflammation-mediated alterations in glutamate neurotransmission constitute the most important pathway in the pathophysiology of various brain disorders. The excessive signalling of glutamate results in excitotoxicity, neuronal degeneration, and neuronal cell death. In the present study, we investig...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Saudi Pharmaceutical Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016422002559 |
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| author | Sibi P Ittiyavirah Kannan Ramalingam Arathy Sathyan R.S. Rajasree Mohamed Saheer Kuruniyan Syed Altafuddin Quadri Muhammed Elayadeth-Meethal Punnoth Poonkuzhi Naseef |
| author_facet | Sibi P Ittiyavirah Kannan Ramalingam Arathy Sathyan R.S. Rajasree Mohamed Saheer Kuruniyan Syed Altafuddin Quadri Muhammed Elayadeth-Meethal Punnoth Poonkuzhi Naseef |
| author_sort | Sibi P Ittiyavirah |
| collection | DOAJ |
| description | Inflammation-mediated alterations in glutamate neurotransmission constitute the most important pathway in the pathophysiology of various brain disorders. The excessive signalling of glutamate results in excitotoxicity, neuronal degeneration, and neuronal cell death. In the present study, we investigated the relative efficacy of black cumin (Nigella sativa) oil with high (5 % w/w) and low (2 % w/w) thymoquinone content (BCO-5 and BCO-2, respectively) in alleviating ibotenic acid-induced excitotoxicity and neuroinflammation in Wistar rats. It was found that BCO-5 reversed the abnormal behavioural patterns and the key inflammatory mediators (TNF-α and NF-κB) when treated at 5 mg/kg body weight. Immunohistochemical studies showed the potential of BCO-5 to attenuate the glutamate receptor subunits NMDA and GluR-2 along with increased glutamate decarboxylase levels in the brain tissues. Histopathological studies revealed the neuroprotection of BCO-5 against the inflammatory lesions, as evidenced by the normal cerebellum, astrocytes, and glial cells. BCO-2 on the other hand showed either a poor protective effect or no effect even at a 4-fold higher concentration of 20 mg/kg body weight indicating a very significant role of thymoquinone content on the neuroprotective effect of black cumin oil and its plausible clinical efficacy in counteracting the anxiety and stress-related neurological disorders under conditions such as depression and Alzheimer's disease. |
| first_indexed | 2024-04-11T05:28:09Z |
| format | Article |
| id | doaj.art-6017143cd40449f0806c693e9942061e |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-04-11T05:28:09Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-6017143cd40449f0806c693e9942061e2022-12-23T04:40:01ZengElsevierSaudi Pharmaceutical Journal1319-01642022-12-01301217811790Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar ratsSibi P Ittiyavirah0Kannan Ramalingam1Arathy Sathyan2R.S. Rajasree3Mohamed Saheer Kuruniyan4Syed Altafuddin Quadri5Muhammed Elayadeth-Meethal6Punnoth Poonkuzhi Naseef7Department of Pharmaceutical Sciences, Centre for Professional and Advanced Sciences, Cheruvandoor, Kottayam 686631, IndiaDepartment of Pharmaceutical Sciences, Centre for Professional and Advanced Sciences, Cheruvandoor, Kottayam 686631, IndiaDepartment of Pharmaceutical Sciences, Centre for Professional and Advanced Sciences, Cheruvandoor, Kottayam 686631, IndiaCollege of Pharmaceutical Sciences, Government Thirumala Devaswom Medical College, Alappuzha 688005, IndiaDepartment of Dental Technology, College of Applied Medical Sciences, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Dental Technology, College of Applied Medical Sciences, King Khalid University, Abha 61421, Saudi ArabiaDepartment of Animal Breeding and Genetics, College of Veterinary and Animal Sciences, Kerala Veterinary and Animal Sciences University, Pookode, Wayanad 675621, IndiaDepartment of Pharmaceutics, Moulana College of Pharmacy, Perinthalmanna 679321, India; Corresponding author.Inflammation-mediated alterations in glutamate neurotransmission constitute the most important pathway in the pathophysiology of various brain disorders. The excessive signalling of glutamate results in excitotoxicity, neuronal degeneration, and neuronal cell death. In the present study, we investigated the relative efficacy of black cumin (Nigella sativa) oil with high (5 % w/w) and low (2 % w/w) thymoquinone content (BCO-5 and BCO-2, respectively) in alleviating ibotenic acid-induced excitotoxicity and neuroinflammation in Wistar rats. It was found that BCO-5 reversed the abnormal behavioural patterns and the key inflammatory mediators (TNF-α and NF-κB) when treated at 5 mg/kg body weight. Immunohistochemical studies showed the potential of BCO-5 to attenuate the glutamate receptor subunits NMDA and GluR-2 along with increased glutamate decarboxylase levels in the brain tissues. Histopathological studies revealed the neuroprotection of BCO-5 against the inflammatory lesions, as evidenced by the normal cerebellum, astrocytes, and glial cells. BCO-2 on the other hand showed either a poor protective effect or no effect even at a 4-fold higher concentration of 20 mg/kg body weight indicating a very significant role of thymoquinone content on the neuroprotective effect of black cumin oil and its plausible clinical efficacy in counteracting the anxiety and stress-related neurological disorders under conditions such as depression and Alzheimer's disease.http://www.sciencedirect.com/science/article/pii/S1319016422002559Black cuminExcitotoxicityIbotenic acidNeuroinflammationNigella sativaThymoquinone |
| spellingShingle | Sibi P Ittiyavirah Kannan Ramalingam Arathy Sathyan R.S. Rajasree Mohamed Saheer Kuruniyan Syed Altafuddin Quadri Muhammed Elayadeth-Meethal Punnoth Poonkuzhi Naseef Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats Saudi Pharmaceutical Journal Black cumin Excitotoxicity Ibotenic acid Neuroinflammation Nigella sativa Thymoquinone |
| title | Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats |
| title_full | Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats |
| title_fullStr | Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats |
| title_full_unstemmed | Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats |
| title_short | Thymoquinone-rich black cumin oil attenuates ibotenic acid-induced excitotoxicity through glutamate receptors in Wistar rats |
| title_sort | thymoquinone rich black cumin oil attenuates ibotenic acid induced excitotoxicity through glutamate receptors in wistar rats |
| topic | Black cumin Excitotoxicity Ibotenic acid Neuroinflammation Nigella sativa Thymoquinone |
| url | http://www.sciencedirect.com/science/article/pii/S1319016422002559 |
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