Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells
Given the increasing interest in bioactive dietary components that can modulate gene expression enhancing human health, three metabolites isolated from hemp seeds—cannabidiolic acid, <i>N</i>-<i>trans</i>-caffeoyltyramine, and cannabisin B—were examined for their ability to c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/44/10/347 |
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| author | Armando Di Palo Chiara Siniscalchi Giuseppina Crescente Ilenia De Leo Antonio Fiorentino Severina Pacifico Aniello Russo Nicoletta Potenza |
| author_facet | Armando Di Palo Chiara Siniscalchi Giuseppina Crescente Ilenia De Leo Antonio Fiorentino Severina Pacifico Aniello Russo Nicoletta Potenza |
| author_sort | Armando Di Palo |
| collection | DOAJ |
| description | Given the increasing interest in bioactive dietary components that can modulate gene expression enhancing human health, three metabolites isolated from hemp seeds—cannabidiolic acid, <i>N</i>-<i>trans</i>-caffeoyltyramine, and cannabisin B—were examined for their ability to change the expression levels of microRNAs in human neural cells. To this end, cultured SH-SY5Y cells were treated with the three compounds and their microRNA content was characterized by next-generation small RNA sequencing. As a result, 31 microRNAs underwent major expression changes, being at least doubled or halved by the treatments. A computational analysis of the biological pathways affected by these microRNAs then showed that some are implicated in neural functions, such as axon guidance, hippocampal signaling, and neurotrophin signaling. Of these, miR-708-5p, miR-181a-5p, miR-190a-5p, miR-199a-5p, and miR-143-3p are known to be involved in Alzheimer’s disease and their expression changes are expected to ameliorate neural function. Overall, these results provide new insights into the mechanism of action of hemp seed metabolites and encourage further studies to gain a better understanding of their biological effects on the central nervous system. |
| first_indexed | 2024-03-09T20:26:06Z |
| format | Article |
| id | doaj.art-601d683d83ac42af8916a13d64c13f35 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-09T20:26:06Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-601d683d83ac42af8916a13d64c13f352023-11-23T23:34:23ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452022-10-0144105106511610.3390/cimb44100347Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural CellsArmando Di Palo0Chiara Siniscalchi1Giuseppina Crescente2Ilenia De Leo3Antonio Fiorentino4Severina Pacifico5Aniello Russo6Nicoletta Potenza7Department of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies—University of Campania “Luigi Vanvitelli”, 81100 Caserta, ItalyGiven the increasing interest in bioactive dietary components that can modulate gene expression enhancing human health, three metabolites isolated from hemp seeds—cannabidiolic acid, <i>N</i>-<i>trans</i>-caffeoyltyramine, and cannabisin B—were examined for their ability to change the expression levels of microRNAs in human neural cells. To this end, cultured SH-SY5Y cells were treated with the three compounds and their microRNA content was characterized by next-generation small RNA sequencing. As a result, 31 microRNAs underwent major expression changes, being at least doubled or halved by the treatments. A computational analysis of the biological pathways affected by these microRNAs then showed that some are implicated in neural functions, such as axon guidance, hippocampal signaling, and neurotrophin signaling. Of these, miR-708-5p, miR-181a-5p, miR-190a-5p, miR-199a-5p, and miR-143-3p are known to be involved in Alzheimer’s disease and their expression changes are expected to ameliorate neural function. Overall, these results provide new insights into the mechanism of action of hemp seed metabolites and encourage further studies to gain a better understanding of their biological effects on the central nervous system.https://www.mdpi.com/1467-3045/44/10/347nutraceuticalsmicroRNAneural cellmiRNomeAlzheimer’s disease |
| spellingShingle | Armando Di Palo Chiara Siniscalchi Giuseppina Crescente Ilenia De Leo Antonio Fiorentino Severina Pacifico Aniello Russo Nicoletta Potenza Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells Current Issues in Molecular Biology nutraceuticals microRNA neural cell miRNome Alzheimer’s disease |
| title | Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells |
| title_full | Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells |
| title_fullStr | Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells |
| title_full_unstemmed | Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells |
| title_short | Effect of Cannabidiolic Acid, <i>N</i>-<i>Trans</i>-Caffeoyltyramine and Cannabisin B from Hemp Seeds on microRNA Expression in Human Neural Cells |
| title_sort | effect of cannabidiolic acid i n i i trans i caffeoyltyramine and cannabisin b from hemp seeds on microrna expression in human neural cells |
| topic | nutraceuticals microRNA neural cell miRNome Alzheimer’s disease |
| url | https://www.mdpi.com/1467-3045/44/10/347 |
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