Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant
Solanum dulcamara L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet w...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2011-01-01
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| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/447 |
| _version_ | 1819026757755338752 |
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| author | Arzu Ucar Turker Esra Canserver Multu |
| author_facet | Arzu Ucar Turker Esra Canserver Multu |
| author_sort | Arzu Ucar Turker |
| collection | DOAJ |
| description | Solanum dulcamara L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various plant growth regulator combinations. Leaf explants formed more shoots than petiole explants. Plant regeneration was observed through indirect organogenesis with both explants. Best shoot proliferation was obtained from leaf explants with 3 mg/l BA (benzyladenine) and 0.5 mg/l IAA (indole-3-acetic acid). Regenerated shoots were transferred to rooting media containing different levels of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid) or 2,4-D (2,4 dichlorophenoxyacetic acid). Most shoots developed roots on medium with 0.5 mg/l IBA. Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 2 weeks, they were planted in plastic pots containing potting soil and maintained in the plant growth room. |
| first_indexed | 2024-12-21T05:31:39Z |
| format | Article |
| id | doaj.art-601f808e4c3f457f99e8d55b799df7ac |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-21T05:31:39Z |
| publishDate | 2011-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-601f808e4c3f457f99e8d55b799df7ac2022-12-21T19:14:31ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802011-01-0177427528010.5586/asbp.2008.034255Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plantArzu Ucar Turker0Esra Canserver Multu1Abant Izzet Baysal UniversityAbant Izzet Baysal UniversitySolanum dulcamara L. (bittersweet) is a medicinal plant that has been used to treat skin diseases, warts, tumors, felons, arthritis, rheumatism, bronchial congestion, heart ailments, ulcerative colitis, eye inflammations, jaundice and pneumonia. A reliable in vitro culture protocol for bittersweet was established. Explants (leaf and petiole segments) were cultured on Murashige and Skoog minimal organics (MSMO) medium with various plant growth regulator combinations. Leaf explants formed more shoots than petiole explants. Plant regeneration was observed through indirect organogenesis with both explants. Best shoot proliferation was obtained from leaf explants with 3 mg/l BA (benzyladenine) and 0.5 mg/l IAA (indole-3-acetic acid). Regenerated shoots were transferred to rooting media containing different levels of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid), NAA (naphthalene acetic acid) or 2,4-D (2,4 dichlorophenoxyacetic acid). Most shoots developed roots on medium with 0.5 mg/l IBA. Rooted explants were transferred to vermiculate in Magenta containers for acclimatization and after 2 weeks, they were planted in plastic pots containing potting soil and maintained in the plant growth room.https://pbsociety.org.pl/journals/index.php/asbp/article/view/447Solanum dulcamarabittersweetcommon nightshadein vitro cultureplant regeneration |
| spellingShingle | Arzu Ucar Turker Esra Canserver Multu Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant Acta Societatis Botanicorum Poloniae Solanum dulcamara bittersweet common nightshade in vitro culture plant regeneration |
| title | Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant |
| title_full | Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant |
| title_fullStr | Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant |
| title_full_unstemmed | Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant |
| title_short | Efficient plant regeneration of bittersweet (Solanum dulcamara L.), a medicinal plant |
| title_sort | efficient plant regeneration of bittersweet solanum dulcamara l a medicinal plant |
| topic | Solanum dulcamara bittersweet common nightshade in vitro culture plant regeneration |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/447 |
| work_keys_str_mv | AT arzuucarturker efficientplantregenerationofbittersweetsolanumdulcamaralamedicinalplant AT esracanservermultu efficientplantregenerationofbittersweetsolanumdulcamaralamedicinalplant |