The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions
Abstract Aims Thymus plant is a very useful herbal medicine with various properties such as anti-inflammatory and antibacterial. Therefore, the properties of this plant have made this drug a suitable candidate for wound healing. In this study, hydroxypropyl methylcellulose (HPMC) gel containing Zata...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-024-04352-1 |
| _version_ | 1797340287026069504 |
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| author | Mahmoud Osanloo Fariba Noori Negar Varaa Alireza Tavassoli Aida Goodarzi Maryam Talebi Moghaddam Lida Ebrahimi Zahra Abpeikar Ahmad Reza Farmani Mohsen Safaei Narges Fereydouni Arash Goodarzi |
| author_facet | Mahmoud Osanloo Fariba Noori Negar Varaa Alireza Tavassoli Aida Goodarzi Maryam Talebi Moghaddam Lida Ebrahimi Zahra Abpeikar Ahmad Reza Farmani Mohsen Safaei Narges Fereydouni Arash Goodarzi |
| author_sort | Mahmoud Osanloo |
| collection | DOAJ |
| description | Abstract Aims Thymus plant is a very useful herbal medicine with various properties such as anti-inflammatory and antibacterial. Therefore, the properties of this plant have made this drug a suitable candidate for wound healing. In this study, hydroxypropyl methylcellulose (HPMC) gel containing Zataria multiflora volatile oil nanoemulsion (neZM) along with polycaprolactone/chitosan (PCL-CS) nanofibrous scaffold was used, and the effect of three experimental groups on the wound healing process was evaluated. The first group, HPMC gel containing neZM, the second group, PCL-CS nanofibers, and the third group, HPMC gel containing neZM and bandaged with PCL-CS nanofibers (PCL-CS/neZM). Wounds bandaged with common sterile gas were considered as control. Methods The nanoemulsion was synthesized by a spontaneous method and loaded into a hydroxypropyl methylcellulose (HPMC) gel. The DLS test investigated the size of these nanoemulsions. A PCL-CS nanofibrous scaffold was also synthesized by electrospinning method then SEM and contact angle tests investigated morphology and hydrophilicity/hydrophobicity of its surface. The animal study was performed on full-thickness skin wounds in rats, and the process of tissue regeneration in the experimental and control groups was evaluated by H&E and Masson's trichrome staining. Results The results showed that the nanoemulsion has a size of 225±9 nm and has an acceptable dispersion. The PCL-CS nanofibers synthesized by the electrospinning method also show non-beaded smooth fibers and due to the presence of chitosan with hydrophilic properties, have higher surface hydrophobicity than PCL fibers. The wound healing results show that the PCL-CS/neZM group significantly reduced the wound size compared to the other groups on the 7th, 14th, and 21st days. The histological results also show that the PCL-CS/neZM group could significantly reduce the parameters of edema, inflammation, and vascularity and increase the parameters of fibrosis, re-epithelialization, and collagen deposition compared to other groups on day 21. Conclusion The results of this study show that the PCL-CS/neZM treatment can effectively improve wound healing. |
| first_indexed | 2024-03-08T10:00:47Z |
| format | Article |
| id | doaj.art-60216218e0604a928984f2ef237cdbb6 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-03-08T10:00:47Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-60216218e0604a928984f2ef237cdbb62024-01-29T10:57:19ZengBMCBMC Complementary Medicine and Therapies2662-76712024-01-0124111610.1186/s12906-024-04352-1The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsionsMahmoud Osanloo0Fariba Noori1Negar Varaa2Alireza Tavassoli3Aida Goodarzi4Maryam Talebi Moghaddam5Lida Ebrahimi6Zahra Abpeikar7Ahmad Reza Farmani8Mohsen Safaei9Narges Fereydouni10Arash Goodarzi11Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesDepartment of Anatomy, School of Medicine, Fasa University of Medical SciencesDepartment of Pathology, School of Medicine, Fasa University of Medical SciencesDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesNoncommunicable Diseases Research Center, Fasa University of Medical SciencesDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesNoncommunicable Diseases Research Center, Fasa University of Medical SciencesDepartment of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical SciencesAbstract Aims Thymus plant is a very useful herbal medicine with various properties such as anti-inflammatory and antibacterial. Therefore, the properties of this plant have made this drug a suitable candidate for wound healing. In this study, hydroxypropyl methylcellulose (HPMC) gel containing Zataria multiflora volatile oil nanoemulsion (neZM) along with polycaprolactone/chitosan (PCL-CS) nanofibrous scaffold was used, and the effect of three experimental groups on the wound healing process was evaluated. The first group, HPMC gel containing neZM, the second group, PCL-CS nanofibers, and the third group, HPMC gel containing neZM and bandaged with PCL-CS nanofibers (PCL-CS/neZM). Wounds bandaged with common sterile gas were considered as control. Methods The nanoemulsion was synthesized by a spontaneous method and loaded into a hydroxypropyl methylcellulose (HPMC) gel. The DLS test investigated the size of these nanoemulsions. A PCL-CS nanofibrous scaffold was also synthesized by electrospinning method then SEM and contact angle tests investigated morphology and hydrophilicity/hydrophobicity of its surface. The animal study was performed on full-thickness skin wounds in rats, and the process of tissue regeneration in the experimental and control groups was evaluated by H&E and Masson's trichrome staining. Results The results showed that the nanoemulsion has a size of 225±9 nm and has an acceptable dispersion. The PCL-CS nanofibers synthesized by the electrospinning method also show non-beaded smooth fibers and due to the presence of chitosan with hydrophilic properties, have higher surface hydrophobicity than PCL fibers. The wound healing results show that the PCL-CS/neZM group significantly reduced the wound size compared to the other groups on the 7th, 14th, and 21st days. The histological results also show that the PCL-CS/neZM group could significantly reduce the parameters of edema, inflammation, and vascularity and increase the parameters of fibrosis, re-epithelialization, and collagen deposition compared to other groups on day 21. Conclusion The results of this study show that the PCL-CS/neZM treatment can effectively improve wound healing.https://doi.org/10.1186/s12906-024-04352-1Wound healingZataria multifloraVolatile Oil nanoemulsionHydroxypropyl methylcellulose (HPMC)Polycaprolactone (PCL)Chitosan |
| spellingShingle | Mahmoud Osanloo Fariba Noori Negar Varaa Alireza Tavassoli Aida Goodarzi Maryam Talebi Moghaddam Lida Ebrahimi Zahra Abpeikar Ahmad Reza Farmani Mohsen Safaei Narges Fereydouni Arash Goodarzi The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions BMC Complementary Medicine and Therapies Wound healing Zataria multiflora Volatile Oil nanoemulsion Hydroxypropyl methylcellulose (HPMC) Polycaprolactone (PCL) Chitosan |
| title | The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions |
| title_full | The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions |
| title_fullStr | The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions |
| title_full_unstemmed | The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions |
| title_short | The wound healing effect of polycaprolactone-chitosan scaffold coated with a gel containing Zataria multiflora Boiss. volatile oil nanoemulsions |
| title_sort | wound healing effect of polycaprolactone chitosan scaffold coated with a gel containing zataria multiflora boiss volatile oil nanoemulsions |
| topic | Wound healing Zataria multiflora Volatile Oil nanoemulsion Hydroxypropyl methylcellulose (HPMC) Polycaprolactone (PCL) Chitosan |
| url | https://doi.org/10.1186/s12906-024-04352-1 |
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