Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. Fruit
The yield and quality of goji (<i>Lycium barbarum</i> L.) fruit are heavily dependent on fertilizer, especially the availability of nitrogen, phosphorus, and potassium (N, P, and K, respectively). In this study, we performed a metabolomic analysis of the response of goji berry to nitroge...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/24/21/3879 |
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| author | Zhigang Shi Feng Wei Ru Wan Yunxiang Li Yajun Wang Wei An Ken Qin Guoli Dai Youlong Cao Jiayue Feng |
| author_facet | Zhigang Shi Feng Wei Ru Wan Yunxiang Li Yajun Wang Wei An Ken Qin Guoli Dai Youlong Cao Jiayue Feng |
| author_sort | Zhigang Shi |
| collection | DOAJ |
| description | The yield and quality of goji (<i>Lycium barbarum</i> L.) fruit are heavily dependent on fertilizer, especially the availability of nitrogen, phosphorus, and potassium (N, P, and K, respectively). In this study, we performed a metabolomic analysis of the response of goji berry to nitrogen fertilizer levels using an Ultra Performance Liquid Chromatography−Electrospray Ionization−Tandem Mass Spectrometry (UPLC-ESI-MS/MS) method. There was no significant difference in the fruit yield or the commodity grade between N0 (42.5 g/plant), N1 (85 g/plant), and N2 (127.5 g/plant). The primary nutrients of the goji berry changed with an increasing nitrogen fertilization. Comparative metabolomic profiling of three nitrogen levels resulted in the identification of 612 metabolites, including amino acids, flavonoids, carbohydrates, organic acids, and lipids/alcohols, among others, of which 53 metabolites (lipids, fatty acids, organic acids, and phenolamides) demonstrated significant changes. These results provide new insights into the molecular mechanisms of the relationship between yield and quality of goji berry and nitrogen fertilizer. |
| first_indexed | 2024-12-13T05:02:30Z |
| format | Article |
| id | doaj.art-6021e7d917b64368bbf4ac2b0bcf3140 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T05:02:30Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-6021e7d917b64368bbf4ac2b0bcf31402022-12-21T23:58:44ZengMDPI AGMolecules1420-30492019-10-012421387910.3390/molecules24213879molecules24213879Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. FruitZhigang Shi0Feng Wei1Ru Wan2Yunxiang Li3Yajun Wang4Wei An5Ken Qin6Guoli Dai7Youlong Cao8Jiayue Feng9Wolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaCollege of Horticulture, Northwest A & F University, Yangling, Xianyang 712000, ChinaWolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaWolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaWolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaWolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaWolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaWolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaWolfberry Engineering Research Institute, Ningxia Academy of Agriculture and Forestry Sciences, National Wolfberry Engineering Research Center, Yinchuan 750002, ChinaCollege of Horticulture, Northwest A & F University, Yangling, Xianyang 712000, ChinaThe yield and quality of goji (<i>Lycium barbarum</i> L.) fruit are heavily dependent on fertilizer, especially the availability of nitrogen, phosphorus, and potassium (N, P, and K, respectively). In this study, we performed a metabolomic analysis of the response of goji berry to nitrogen fertilizer levels using an Ultra Performance Liquid Chromatography−Electrospray Ionization−Tandem Mass Spectrometry (UPLC-ESI-MS/MS) method. There was no significant difference in the fruit yield or the commodity grade between N0 (42.5 g/plant), N1 (85 g/plant), and N2 (127.5 g/plant). The primary nutrients of the goji berry changed with an increasing nitrogen fertilization. Comparative metabolomic profiling of three nitrogen levels resulted in the identification of 612 metabolites, including amino acids, flavonoids, carbohydrates, organic acids, and lipids/alcohols, among others, of which 53 metabolites (lipids, fatty acids, organic acids, and phenolamides) demonstrated significant changes. These results provide new insights into the molecular mechanisms of the relationship between yield and quality of goji berry and nitrogen fertilizer.https://www.mdpi.com/1420-3049/24/21/3879<i>lycium barbarum</i> l.nitrogenmetabolomeyieldquality |
| spellingShingle | Zhigang Shi Feng Wei Ru Wan Yunxiang Li Yajun Wang Wei An Ken Qin Guoli Dai Youlong Cao Jiayue Feng Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. Fruit Molecules <i>lycium barbarum</i> l. nitrogen metabolome yield quality |
| title | Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. Fruit |
| title_full | Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. Fruit |
| title_fullStr | Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. Fruit |
| title_full_unstemmed | Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. Fruit |
| title_short | Impact of Nitrogen Fertilizer Levels on Metabolite Profiling of the <i>Lycium barbarum</i> L. Fruit |
| title_sort | impact of nitrogen fertilizer levels on metabolite profiling of the i lycium barbarum i l fruit |
| topic | <i>lycium barbarum</i> l. nitrogen metabolome yield quality |
| url | https://www.mdpi.com/1420-3049/24/21/3879 |
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