Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control Study
Introduction: Psoriasis is a chronic inflammatory disease distributed worldwide with varying prevalence among different geographical areas and ethnic groups. It has been recently found that oxidative stress is one of the important factors in the pathogenesis of psoriasis. The varied effects of oxida...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2022-12-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://www.jcdr.net/articles/PDF/17186/60052_CE(AD)_F[SH]_PF1(AG_OM)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdf |
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| author | UG Bhavyashree Laddha Vedant B Vishal Malcolm Pinto Manjunath Shenoy |
| author_facet | UG Bhavyashree Laddha Vedant B Vishal Malcolm Pinto Manjunath Shenoy |
| author_sort | UG Bhavyashree |
| collection | DOAJ |
| description | Introduction: Psoriasis is a chronic inflammatory disease distributed worldwide with varying prevalence among different geographical areas and ethnic groups. It has been recently found that oxidative stress is one of the important factors in the pathogenesis of psoriasis. The varied effects of oxidative stress include changes in cellular uptake, altered enzymatic activity of proteins, increased predisposition to aggregation and proteolysis, which subsequently alter their immunogenicity. Sialic Acid (SA) is an acetylated derivative of neuramic acid. It is a marker for acute phase inflammatory response, with increased levels observed in inflammatory diseases.
Aim: To estimate the levels of SA in psoriasis patients and to correlate with the severity of the disease.
Materials and Methods: This case-control study was conducted among 50 patients, diagnosed with psoriasis, and 50 age and sex-matched subjects without psoriasis. General, systemic and dermatological examination was carried out. The severity of psoriasis was assessed according to Psoriasis Area Severity Index (PASI). The serum was treated with ethanol and centrifugation to precipitate proteins. The SA of both the precipitate and supernatant was estimated based on the reaction of SA with the ninhydrin reagent. Calculation of sialic acid was done by the formula: OD of test/OD of standard×concentration of the standard. To establish a correlation student’s t-test was used.
Results: There were 39 males and 11 females in each case and control group. The mean±SD age of cases and controls was 44.04±10.9 years and 44.10±9.996, respectively. The mean PASI value was 23.1666±18.47. Mean SA in cases and controls were 35.792±2.124 and 28.556±3.854 (p-values <0.001). A positive correlation was observed between the free SA and psoriasis severity (p-value <0.001, r=0.460).
Conclusion: Higher levels of free serum SA were significantly associated with more severe forms of psoriasis. |
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| format | Article |
| id | doaj.art-602264785cb24532b14243959596fd51 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-04-10T20:51:17Z |
| publishDate | 2022-12-01 |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-602264785cb24532b14243959596fd512023-01-23T11:12:11ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2022-12-011612WC01WC0310.7860/JCDR/2022/60052.17186Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control StudyUG Bhavyashree0Laddha Vedant1B Vishal2Malcolm Pinto3Manjunath Shenoy4Associate Consultant, Department of Dermatology, Specialist’s Hospital, Kalyaninagar, Bangalore, Karnataka, India.Postgraduate, Department of Dermatology, Yenepoya Medical College, Mangalore, Karnataka, India.Professor, Department of Dermatology, Yenepoya Medical College, Mangalore, Karnataka, India.Associate Professor, Department of Dermatology, Yenepoya Medical College, Mangalore, Karnataka, India.Professor and Head, Department of Dermatology, Yenepoya Medical College, Mangalore, Karnataka, India.Introduction: Psoriasis is a chronic inflammatory disease distributed worldwide with varying prevalence among different geographical areas and ethnic groups. It has been recently found that oxidative stress is one of the important factors in the pathogenesis of psoriasis. The varied effects of oxidative stress include changes in cellular uptake, altered enzymatic activity of proteins, increased predisposition to aggregation and proteolysis, which subsequently alter their immunogenicity. Sialic Acid (SA) is an acetylated derivative of neuramic acid. It is a marker for acute phase inflammatory response, with increased levels observed in inflammatory diseases. Aim: To estimate the levels of SA in psoriasis patients and to correlate with the severity of the disease. Materials and Methods: This case-control study was conducted among 50 patients, diagnosed with psoriasis, and 50 age and sex-matched subjects without psoriasis. General, systemic and dermatological examination was carried out. The severity of psoriasis was assessed according to Psoriasis Area Severity Index (PASI). The serum was treated with ethanol and centrifugation to precipitate proteins. The SA of both the precipitate and supernatant was estimated based on the reaction of SA with the ninhydrin reagent. Calculation of sialic acid was done by the formula: OD of test/OD of standard×concentration of the standard. To establish a correlation student’s t-test was used. Results: There were 39 males and 11 females in each case and control group. The mean±SD age of cases and controls was 44.04±10.9 years and 44.10±9.996, respectively. The mean PASI value was 23.1666±18.47. Mean SA in cases and controls were 35.792±2.124 and 28.556±3.854 (p-values <0.001). A positive correlation was observed between the free SA and psoriasis severity (p-value <0.001, r=0.460). Conclusion: Higher levels of free serum SA were significantly associated with more severe forms of psoriasis.https://www.jcdr.net/articles/PDF/17186/60052_CE(AD)_F[SH]_PF1(AG_OM)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdfinflammatory diseaseimmunogenicitypsoriasis area severity index |
| spellingShingle | UG Bhavyashree Laddha Vedant B Vishal Malcolm Pinto Manjunath Shenoy Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control Study Journal of Clinical and Diagnostic Research inflammatory disease immunogenicity psoriasis area severity index |
| title | Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control Study |
| title_full | Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control Study |
| title_fullStr | Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control Study |
| title_full_unstemmed | Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control Study |
| title_short | Association between Serum Sialic Acid Levels and Disease Severity in Psoriasis: A Case-control Study |
| title_sort | association between serum sialic acid levels and disease severity in psoriasis a case control study |
| topic | inflammatory disease immunogenicity psoriasis area severity index |
| url | https://www.jcdr.net/articles/PDF/17186/60052_CE(AD)_F[SH]_PF1(AG_OM)_PFA(SHU)_PB(AG_SHU)_PN(SHU).pdf |
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