Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress
High-carbohydrate (HC) diets decrease the intestinal levels of sodium acetate (SA) and sodium butyrate (SB) and impair the gut health of largemouth bass; however, SA and SB have been shown to enhance immunity and improve intestinal health in farmed animals. Thus, the present study was to investigate...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Animal Nutrition |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405654523001622 |
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| author | Liulan Zhao Liangshun Cheng Yifang Hu Xiaohui Li Yihui Yang Jin Mu Lianfeng Shen Guojun Hu Kuo He Haoxiao Yan Qiao Liu Song Yang |
| author_facet | Liulan Zhao Liangshun Cheng Yifang Hu Xiaohui Li Yihui Yang Jin Mu Lianfeng Shen Guojun Hu Kuo He Haoxiao Yan Qiao Liu Song Yang |
| author_sort | Liulan Zhao |
| collection | DOAJ |
| description | High-carbohydrate (HC) diets decrease the intestinal levels of sodium acetate (SA) and sodium butyrate (SB) and impair the gut health of largemouth bass; however, SA and SB have been shown to enhance immunity and improve intestinal health in farmed animals. Thus, the present study was to investigate the effects of dietary SA and SB on HC diet-induced intestinal injury and the potential mechanisms in juvenile largemouth bass. The experiment set five isonitrogenous and isolipidic diets, including a low-carbohydrate diet (9% starch) (LC), a high carbohydrate diet (18% starch) (HC), and the HC diet supplemented with 2 g/kg SA (HCSA), 2 g/kg SB (HCSB) or a combination of 1 g/kg SA and 1 g/kg SB (HCSASB). The feeding experiment was conducted for 8 weeks. A total of 525 juvenile largemouth bass with an initial body weight of 7.00 ± 0.20 g were used. The results showed that dietary SA and SB improved the weight gain rate and specific growth rate (P < 0.05) and ameliorated serum parameters (alkaline phosphatase, acid phosphatase, glutamate transaminase, and glutamic oxaloacetic transaminase) (P < 0.05). And, importantly, dietary SA and SB repaired the intestinal barrier by increasing the expression levels of zonula occludens-1, occludin, and claudin-7 (P < 0.05), reduced HC-induced intestinal damage, and alleviated intestinal inflammation and cell apoptosis by attenuating HC-induced intestinal endoplasmic reticulum stress (P < 0.05). Further results revealed that dietary SA and SB reduced HC-induced intestinal fat deposition by inhibiting adipogenesis and promoting lipolysis (P < 0.05). In summary, this study demonstrated that dietary SA and SB attenuated HC-induced intestinal damage and reduced excessive intestinal fat deposition in largemouth bass. |
| first_indexed | 2024-03-07T22:01:08Z |
| format | Article |
| id | doaj.art-602418a09e884664979fea12135a56a8 |
| institution | Directory Open Access Journal |
| issn | 2405-6545 |
| language | English |
| last_indexed | 2024-03-07T22:01:08Z |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Animal Nutrition |
| spelling | doaj.art-602418a09e884664979fea12135a56a82024-02-24T04:55:01ZengKeAi Communications Co., Ltd.Animal Nutrition2405-65452024-03-0116443456Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stressLiulan Zhao0Liangshun Cheng1Yifang Hu2Xiaohui Li3Yihui Yang4Jin Mu5Lianfeng Shen6Guojun Hu7Kuo He8Haoxiao Yan9Qiao Liu10Song Yang11College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCollege of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCorresponding authors.; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaCorresponding authors.; College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan, 611130, ChinaHigh-carbohydrate (HC) diets decrease the intestinal levels of sodium acetate (SA) and sodium butyrate (SB) and impair the gut health of largemouth bass; however, SA and SB have been shown to enhance immunity and improve intestinal health in farmed animals. Thus, the present study was to investigate the effects of dietary SA and SB on HC diet-induced intestinal injury and the potential mechanisms in juvenile largemouth bass. The experiment set five isonitrogenous and isolipidic diets, including a low-carbohydrate diet (9% starch) (LC), a high carbohydrate diet (18% starch) (HC), and the HC diet supplemented with 2 g/kg SA (HCSA), 2 g/kg SB (HCSB) or a combination of 1 g/kg SA and 1 g/kg SB (HCSASB). The feeding experiment was conducted for 8 weeks. A total of 525 juvenile largemouth bass with an initial body weight of 7.00 ± 0.20 g were used. The results showed that dietary SA and SB improved the weight gain rate and specific growth rate (P < 0.05) and ameliorated serum parameters (alkaline phosphatase, acid phosphatase, glutamate transaminase, and glutamic oxaloacetic transaminase) (P < 0.05). And, importantly, dietary SA and SB repaired the intestinal barrier by increasing the expression levels of zonula occludens-1, occludin, and claudin-7 (P < 0.05), reduced HC-induced intestinal damage, and alleviated intestinal inflammation and cell apoptosis by attenuating HC-induced intestinal endoplasmic reticulum stress (P < 0.05). Further results revealed that dietary SA and SB reduced HC-induced intestinal fat deposition by inhibiting adipogenesis and promoting lipolysis (P < 0.05). In summary, this study demonstrated that dietary SA and SB attenuated HC-induced intestinal damage and reduced excessive intestinal fat deposition in largemouth bass.http://www.sciencedirect.com/science/article/pii/S2405654523001622Sodium acetateSodium butyrateHigh carbohydrate dietEndoplasmic reticulum stressIntestine healthLipid metabolism |
| spellingShingle | Liulan Zhao Liangshun Cheng Yifang Hu Xiaohui Li Yihui Yang Jin Mu Lianfeng Shen Guojun Hu Kuo He Haoxiao Yan Qiao Liu Song Yang Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress Animal Nutrition Sodium acetate Sodium butyrate High carbohydrate diet Endoplasmic reticulum stress Intestine health Lipid metabolism |
| title | Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress |
| title_full | Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress |
| title_fullStr | Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress |
| title_full_unstemmed | Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress |
| title_short | Dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass (Micropterus salmoides) fed a high carbohydrate diet by reducing endoplasmic reticulum stress |
| title_sort | dietary sodium acetate and sodium butyrate attenuate intestinal damage and improve lipid metabolism in juvenile largemouth bass micropterus salmoides fed a high carbohydrate diet by reducing endoplasmic reticulum stress |
| topic | Sodium acetate Sodium butyrate High carbohydrate diet Endoplasmic reticulum stress Intestine health Lipid metabolism |
| url | http://www.sciencedirect.com/science/article/pii/S2405654523001622 |
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