Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signalling
Context Curcumin, a polyphenolic compound extracted from the rhizome of the tropical plant Curcuma longa L. (Zingiberaceae), has been considered as a cancer chemopreventive drug by American National Cancer Institute. Objective To examine the effect of curcumin on acute monocytic leukaemia SHI-1 cell...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2019.1701042 |
| _version_ | 1818670221069647872 |
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| author | Guohua Zhu Qun Shen Hong Jiang Ou Ji Lingling Zhu Linyang Zhang |
| author_facet | Guohua Zhu Qun Shen Hong Jiang Ou Ji Lingling Zhu Linyang Zhang |
| author_sort | Guohua Zhu |
| collection | DOAJ |
| description | Context Curcumin, a polyphenolic compound extracted from the rhizome of the tropical plant Curcuma longa L. (Zingiberaceae), has been considered as a cancer chemopreventive drug by American National Cancer Institute. Objective To examine the effect of curcumin on acute monocytic leukaemia SHI-1 cells in vivo. Materials and methods The SHI-1 cells (1 × 106 cells in 0.1 mL PBS) were injected subcutaneously into the right flanks of the female SCID mice. Curcumin dissolved in olive oil (15 and 30 mg/kg) was administered (i.p.) to mice once a day for 15 days while the control group received olive oil injection. Tumour proliferation and apoptosis were examined by PCNA, TUNEL and cleaved caspase-3 staining. The expression of MAPK, NF-κB, MMP9, MMP2 and vimentin were confirmed by RT-PCR, immunohistochemistry or western blotting. Results Administration of curcumin significantly inhibited tumour growth, as the tumour weight decreased from 0.67 g (control) to 0.47 g (15 mg/kg) and 0.35 g (30 mg/kg). Curcumin inhibited the expression of PCNA and increased the degree of TUNEL and cleaved caspase-3 staining in tumour tissue. The results of western blotting showed that curcumin treatment inhibited NF-κB and ERK signalling while activating p38 and JNK. Moreover, curcumin attenuated the mRNA transcription and protein expression of MMP2 and MMP9. Curcumin also suppressed the level of vimentin. Discussion and conclusions Our study demonstrates that curcumin can inhibit the growth and invasion of human monocytic leukaemia in vivo, suggesting the possible use of curcumin for anti-metastasis in leukaemia and the value of determining its unique target. |
| first_indexed | 2024-12-17T07:04:39Z |
| format | Article |
| id | doaj.art-60289578bb464477bd786a6569b7bcdf |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-17T07:04:39Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-60289578bb464477bd786a6569b7bcdf2022-12-21T21:59:13ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162020-01-01581253410.1080/13880209.2019.17010421701042Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signallingGuohua Zhu0Qun Shen1Hong Jiang2Ou Ji3Lingling Zhu4Linyang Zhang5First Clinical College, Nanjing University of Chinese MedicineFirst Clinical College, Nanjing University of Chinese MedicineFirst Clinical College, Nanjing University of Chinese MedicineFirst Clinical College, Nanjing University of Chinese MedicineFirst Clinical College, Nanjing University of Chinese MedicineFirst Clinical College, Nanjing University of Chinese MedicineContext Curcumin, a polyphenolic compound extracted from the rhizome of the tropical plant Curcuma longa L. (Zingiberaceae), has been considered as a cancer chemopreventive drug by American National Cancer Institute. Objective To examine the effect of curcumin on acute monocytic leukaemia SHI-1 cells in vivo. Materials and methods The SHI-1 cells (1 × 106 cells in 0.1 mL PBS) were injected subcutaneously into the right flanks of the female SCID mice. Curcumin dissolved in olive oil (15 and 30 mg/kg) was administered (i.p.) to mice once a day for 15 days while the control group received olive oil injection. Tumour proliferation and apoptosis were examined by PCNA, TUNEL and cleaved caspase-3 staining. The expression of MAPK, NF-κB, MMP9, MMP2 and vimentin were confirmed by RT-PCR, immunohistochemistry or western blotting. Results Administration of curcumin significantly inhibited tumour growth, as the tumour weight decreased from 0.67 g (control) to 0.47 g (15 mg/kg) and 0.35 g (30 mg/kg). Curcumin inhibited the expression of PCNA and increased the degree of TUNEL and cleaved caspase-3 staining in tumour tissue. The results of western blotting showed that curcumin treatment inhibited NF-κB and ERK signalling while activating p38 and JNK. Moreover, curcumin attenuated the mRNA transcription and protein expression of MMP2 and MMP9. Curcumin also suppressed the level of vimentin. Discussion and conclusions Our study demonstrates that curcumin can inhibit the growth and invasion of human monocytic leukaemia in vivo, suggesting the possible use of curcumin for anti-metastasis in leukaemia and the value of determining its unique target.http://dx.doi.org/10.1080/13880209.2019.1701042acute myeloid leukaemiametastasismatrix metalloproteinasemitogen-activated protein kinase |
| spellingShingle | Guohua Zhu Qun Shen Hong Jiang Ou Ji Lingling Zhu Linyang Zhang Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signalling Pharmaceutical Biology acute myeloid leukaemia metastasis matrix metalloproteinase mitogen-activated protein kinase |
| title | Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signalling |
| title_full | Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signalling |
| title_fullStr | Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signalling |
| title_full_unstemmed | Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signalling |
| title_short | Curcumin inhibited the growth and invasion of human monocytic leukaemia SHI-1 cells in vivo by altering MAPK and MMP signalling |
| title_sort | curcumin inhibited the growth and invasion of human monocytic leukaemia shi 1 cells in vivo by altering mapk and mmp signalling |
| topic | acute myeloid leukaemia metastasis matrix metalloproteinase mitogen-activated protein kinase |
| url | http://dx.doi.org/10.1080/13880209.2019.1701042 |
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