Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A.
The UNC-104/KIF1A motor is crucial for axonal transport of synaptic vesicles, but how the UNC-104/KIF1A motor is activated in vivo is not fully understood. Here, we identified point mutations located in the motor domain or the inhibitory CC1 domain, which resulted in gain-of-function alleles of unc-...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-11-01
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| Series: | PLoS Genetics |
| Online Access: | https://doi.org/10.1371/journal.pgen.1009940 |
| _version_ | 1818366307317317632 |
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| author | Dezi Cong Jinqi Ren Yurong Zhou Shuang Wang Jingjing Liang Mei Ding Wei Feng |
| author_facet | Dezi Cong Jinqi Ren Yurong Zhou Shuang Wang Jingjing Liang Mei Ding Wei Feng |
| author_sort | Dezi Cong |
| collection | DOAJ |
| description | The UNC-104/KIF1A motor is crucial for axonal transport of synaptic vesicles, but how the UNC-104/KIF1A motor is activated in vivo is not fully understood. Here, we identified point mutations located in the motor domain or the inhibitory CC1 domain, which resulted in gain-of-function alleles of unc-104 that exhibit hyperactive axonal transport and abnormal accumulation of synaptic vesicles. In contrast to the cell body localization of wild type motor, the mutant motors accumulate on neuronal processes. Once on the neuronal process, the mutant motors display dynamic movement similarly to wild type motors. The gain-of-function mutation on the motor domain leads to an active dimeric conformation, releasing the inhibitory CC1 region from the motor domain. Genetically engineered mutations in the motor domain or CC1 of UNC-104, which disrupt the autoinhibitory interface, also led to the gain of function and hyperactivation of axonal transport. Thus, the CC1/motor domain-mediated autoinhibition is crucial for UNC-104/KIF1A-mediated axonal transport in vivo. |
| first_indexed | 2024-12-13T22:34:05Z |
| format | Article |
| id | doaj.art-6028e467ac2b48d5a2dcd198968f1a82 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-13T22:34:05Z |
| publishDate | 2021-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-6028e467ac2b48d5a2dcd198968f1a822022-12-21T23:29:01ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042021-11-011711e100994010.1371/journal.pgen.1009940Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A.Dezi CongJinqi RenYurong ZhouShuang WangJingjing LiangMei DingWei FengThe UNC-104/KIF1A motor is crucial for axonal transport of synaptic vesicles, but how the UNC-104/KIF1A motor is activated in vivo is not fully understood. Here, we identified point mutations located in the motor domain or the inhibitory CC1 domain, which resulted in gain-of-function alleles of unc-104 that exhibit hyperactive axonal transport and abnormal accumulation of synaptic vesicles. In contrast to the cell body localization of wild type motor, the mutant motors accumulate on neuronal processes. Once on the neuronal process, the mutant motors display dynamic movement similarly to wild type motors. The gain-of-function mutation on the motor domain leads to an active dimeric conformation, releasing the inhibitory CC1 region from the motor domain. Genetically engineered mutations in the motor domain or CC1 of UNC-104, which disrupt the autoinhibitory interface, also led to the gain of function and hyperactivation of axonal transport. Thus, the CC1/motor domain-mediated autoinhibition is crucial for UNC-104/KIF1A-mediated axonal transport in vivo.https://doi.org/10.1371/journal.pgen.1009940 |
| spellingShingle | Dezi Cong Jinqi Ren Yurong Zhou Shuang Wang Jingjing Liang Mei Ding Wei Feng Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A. PLoS Genetics |
| title | Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A. |
| title_full | Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A. |
| title_fullStr | Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A. |
| title_full_unstemmed | Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A. |
| title_short | Motor domain-mediated autoinhibition dictates axonal transport by the kinesin UNC-104/KIF1A. |
| title_sort | motor domain mediated autoinhibition dictates axonal transport by the kinesin unc 104 kif1a |
| url | https://doi.org/10.1371/journal.pgen.1009940 |
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