Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled Crops
The aim of the study is to evaluate the composition of lyophilisates obtained from <i>Aloe arborescens</i> leaf gel at the age of one to four years. The leaves were obtained from controlled crops, which allowed to exclude environmental factors as variables. It was confirmed that the lyop...
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MDPI AG
2021-05-01
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author | Kamil Pawłowicz Dominika Ludowicz Marta Karaźniewicz-Łada Kamil Wdowiak Judyta Cielecka-Piontek |
author_facet | Kamil Pawłowicz Dominika Ludowicz Marta Karaźniewicz-Łada Kamil Wdowiak Judyta Cielecka-Piontek |
author_sort | Kamil Pawłowicz |
collection | DOAJ |
description | The aim of the study is to evaluate the composition of lyophilisates obtained from <i>Aloe arborescens</i> leaf gel at the age of one to four years. The leaves were obtained from controlled crops, which allowed to exclude environmental factors as variables. It was confirmed that the lyophilisates obtained from different years of <i>Aloe arborescens</i> leaf gel varied in chromatographic analyses in terms of aloin A and aloenin A content (high-performance liquid chromatography with diode-array detection HPLC-DAD, high-performance liquid chromatography with tandem mass spectrometric detection HPLC-MS/MS). Similarly, while testing the phenolic acids and the sum of polyphenols content, differences in their levels in leaf gel lyophilisates from plants of individual years were observed (spectrophotometric method UV-VIS). The lyophilisate composition analysis showed that the one-year-old leaves were characterized by the highest content of aloin A and aloenin A. While the content of polyphenols, including phenolic acids, was higher in the leaves of older plants. The antioxidant potential of the tested lyophilisates was assessed simultaneously. Regardless of the research model used (CUPRAC, DPPH, ABTS), an antioxidant effect was noted for <i>Aloe arborescens</i> leaves. |
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language | English |
last_indexed | 2024-03-10T10:59:26Z |
publishDate | 2021-05-01 |
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spelling | doaj.art-60292be8d3a44d54bd6dc11c7fa96f7f2023-11-21T21:35:22ZengMDPI AGMolecules1420-30492021-05-012611320410.3390/molecules26113204Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled CropsKamil Pawłowicz0Dominika Ludowicz1Marta Karaźniewicz-Łada2Kamil Wdowiak3Judyta Cielecka-Piontek4Phytopharm Klęka S.A., Klęka 1, 63-040 Nowe Miasto nad Warta, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Poznan University of Medical Sciences, Święcickiego 4, 60-781 Poznań, PolandDepartment of Physical Pharmacy and Pharmacokinetics, Faculty of Pharmacy, Poznan University of Medical Sciences, Święcickiego 6, 60-781 Poznań, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Poznan University of Medical Sciences, Święcickiego 4, 60-781 Poznań, PolandDepartment of Pharmacognosy, Faculty of Pharmacy, Poznan University of Medical Sciences, Święcickiego 4, 60-781 Poznań, PolandThe aim of the study is to evaluate the composition of lyophilisates obtained from <i>Aloe arborescens</i> leaf gel at the age of one to four years. The leaves were obtained from controlled crops, which allowed to exclude environmental factors as variables. It was confirmed that the lyophilisates obtained from different years of <i>Aloe arborescens</i> leaf gel varied in chromatographic analyses in terms of aloin A and aloenin A content (high-performance liquid chromatography with diode-array detection HPLC-DAD, high-performance liquid chromatography with tandem mass spectrometric detection HPLC-MS/MS). Similarly, while testing the phenolic acids and the sum of polyphenols content, differences in their levels in leaf gel lyophilisates from plants of individual years were observed (spectrophotometric method UV-VIS). The lyophilisate composition analysis showed that the one-year-old leaves were characterized by the highest content of aloin A and aloenin A. While the content of polyphenols, including phenolic acids, was higher in the leaves of older plants. The antioxidant potential of the tested lyophilisates was assessed simultaneously. Regardless of the research model used (CUPRAC, DPPH, ABTS), an antioxidant effect was noted for <i>Aloe arborescens</i> leaves.https://www.mdpi.com/1420-3049/26/11/3204<i>Aloe arborescens</i>aloin Aaloenin Apolyphenolsantioxidant potential |
spellingShingle | Kamil Pawłowicz Dominika Ludowicz Marta Karaźniewicz-Łada Kamil Wdowiak Judyta Cielecka-Piontek Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled Crops Molecules <i>Aloe arborescens</i> aloin A aloenin A polyphenols antioxidant potential |
title | Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled Crops |
title_full | Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled Crops |
title_fullStr | Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled Crops |
title_full_unstemmed | Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled Crops |
title_short | Analysis of the Composition of Lyophilisates Obtained from <i>Aloe arborescens</i> Gel of Leaves of Different Ages from Controlled Crops |
title_sort | analysis of the composition of lyophilisates obtained from i aloe arborescens i gel of leaves of different ages from controlled crops |
topic | <i>Aloe arborescens</i> aloin A aloenin A polyphenols antioxidant potential |
url | https://www.mdpi.com/1420-3049/26/11/3204 |
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