Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia
Objective: To study the prevalence of chromosomal anomalies in infertile males with severe oligozoospermia or non obstructive azospermia and its correlation with clinical and endocrine profile. Patients and methods: Consecutive 30 male subjects (mean age 35.5 ± 7.1 years) with primary infertility at...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2018-09-01
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| Series: | African Journal of Urology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1110570418300481 |
| _version_ | 1828412547358785536 |
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| author | M.H. Ali M. Soliman A. Metwally A. Ghobeish |
| author_facet | M.H. Ali M. Soliman A. Metwally A. Ghobeish |
| author_sort | M.H. Ali |
| collection | DOAJ |
| description | Objective: To study the prevalence of chromosomal anomalies in infertile males with severe oligozoospermia or non obstructive azospermia and its correlation with clinical and endocrine profile. Patients and methods: Consecutive 30 male subjects (mean age 35.5 ± 7.1 years) with primary infertility attending at the infertility clinic, Urology department, Suez Canal University Hospital, Egypt were enrolled in the study. These patients had severe oligozoospermia (n = 9) or non obstructive azospermia (n = 21). Clinically testicular volume, scrotal Doppler ultrasound examination and endocrine evaluation (serum FSH, testosterone and prolactin) were determined. Cytogenetic analysis was performed by using the GTG (G-banded using trypsin and Giemsa) banding technique. Results: Nine patients (30%) had chromosomal abnormality. Patients with Klinefelter Syndrome and de la Chapelle male syndrome represented 26.7% (n = 8) and 3.3% (n = 1) respectively. All patients diagnosed as Klinefelter group were azoospermic, while 57.1% of normal karyotyping were azoospermic and 42.9% were severe oligozoospermic (p = 0.029). Klinefelter group had significantly lower mean testosterone level than normal karyotyping group (p = 0.016). Also, Klinefelter group had significantly higher mean FSH and LH levels than normal karyotyping group (p < 0.01). The anomaly detected was 47, XXY chromosomal constitution, found in 8 (38%) out of 21 patients with non-obstructive azoospermia. Conclusion: There is a high prevalence of chromosomal abnormalities in infertile males with non obstructive azoospermia. All patients with azoospermia and severe oligozoospermia (sperm count <5 million/ml) should undergo genetic screening. Our study indicates that even those presenting to infertility clinics can be heterogeneous in terms of karyotype and phenotype. Keywords: Genetics, Infertility, Azoospermia, Karyotyping, Oligozoospermia |
| first_indexed | 2024-12-10T12:51:14Z |
| format | Article |
| id | doaj.art-602af4fdfcc04962b6ef96d678bee90f |
| institution | Directory Open Access Journal |
| issn | 1110-5704 |
| language | English |
| last_indexed | 2024-12-10T12:51:14Z |
| publishDate | 2018-09-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | African Journal of Urology |
| spelling | doaj.art-602af4fdfcc04962b6ef96d678bee90f2022-12-22T01:48:14ZengSpringerOpenAfrican Journal of Urology1110-57042018-09-01243212215Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermiaM.H. Ali0M. Soliman1A. Metwally2A. Ghobeish3Urology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt; Corresponding author.Ismailia General Hospital, Ismailia, EgyptUrology Department, Faculty of Medicine, Suez Canal University, Ismailia, EgyptIsmailia General Hospital, Ismailia, EgyptObjective: To study the prevalence of chromosomal anomalies in infertile males with severe oligozoospermia or non obstructive azospermia and its correlation with clinical and endocrine profile. Patients and methods: Consecutive 30 male subjects (mean age 35.5 ± 7.1 years) with primary infertility attending at the infertility clinic, Urology department, Suez Canal University Hospital, Egypt were enrolled in the study. These patients had severe oligozoospermia (n = 9) or non obstructive azospermia (n = 21). Clinically testicular volume, scrotal Doppler ultrasound examination and endocrine evaluation (serum FSH, testosterone and prolactin) were determined. Cytogenetic analysis was performed by using the GTG (G-banded using trypsin and Giemsa) banding technique. Results: Nine patients (30%) had chromosomal abnormality. Patients with Klinefelter Syndrome and de la Chapelle male syndrome represented 26.7% (n = 8) and 3.3% (n = 1) respectively. All patients diagnosed as Klinefelter group were azoospermic, while 57.1% of normal karyotyping were azoospermic and 42.9% were severe oligozoospermic (p = 0.029). Klinefelter group had significantly lower mean testosterone level than normal karyotyping group (p = 0.016). Also, Klinefelter group had significantly higher mean FSH and LH levels than normal karyotyping group (p < 0.01). The anomaly detected was 47, XXY chromosomal constitution, found in 8 (38%) out of 21 patients with non-obstructive azoospermia. Conclusion: There is a high prevalence of chromosomal abnormalities in infertile males with non obstructive azoospermia. All patients with azoospermia and severe oligozoospermia (sperm count <5 million/ml) should undergo genetic screening. Our study indicates that even those presenting to infertility clinics can be heterogeneous in terms of karyotype and phenotype. Keywords: Genetics, Infertility, Azoospermia, Karyotyping, Oligozoospermiahttp://www.sciencedirect.com/science/article/pii/S1110570418300481 |
| spellingShingle | M.H. Ali M. Soliman A. Metwally A. Ghobeish Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia African Journal of Urology |
| title | Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia |
| title_full | Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia |
| title_fullStr | Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia |
| title_full_unstemmed | Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia |
| title_short | Cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia |
| title_sort | cytogenetic analysis and endocrine profile in patients with nonobstructive azoospermia or severe oligozoospermia |
| url | http://www.sciencedirect.com/science/article/pii/S1110570418300481 |
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